##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512161_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1928239 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.614034878456458 31.0 30.0 34.0 26.0 34.0 2 29.839563975212616 31.0 30.0 34.0 26.0 34.0 3 29.887481271771808 31.0 30.0 34.0 26.0 34.0 4 33.47760210222903 35.0 33.0 37.0 28.0 37.0 5 32.9861101243155 35.0 33.0 37.0 26.0 37.0 6 32.9021703222474 35.0 33.0 37.0 26.0 37.0 7 32.666382123792744 35.0 33.0 37.0 25.0 37.0 8 32.52966618764582 35.0 33.0 37.0 25.0 37.0 9 33.82736164967102 37.0 33.0 39.0 25.0 39.0 10 33.560039497178515 37.0 33.0 39.0 23.0 39.0 11 33.564039001389354 37.0 33.0 39.0 23.0 39.0 12 33.31654997124319 37.0 33.0 39.0 22.0 39.0 13 33.29151987901915 37.0 33.0 39.0 22.0 39.0 14 34.03740978167126 38.0 33.0 40.0 19.0 41.0 15 33.946955745631115 38.0 33.0 40.0 19.0 41.0 16 33.811697616322455 38.0 33.0 40.0 18.0 41.0 17 33.724635794629194 38.0 33.0 40.0 17.0 41.0 18 33.63956853896224 38.0 33.0 40.0 17.0 41.0 19 33.670552768614264 38.0 33.0 40.0 18.0 41.0 20 33.49982652565372 38.0 32.0 40.0 18.0 41.0 21 33.354555114796455 38.0 32.0 40.0 16.0 41.0 22 33.430559697215955 38.0 33.0 40.0 11.0 41.0 23 33.27758955191758 38.0 33.0 40.0 10.0 41.0 24 33.08059218800159 38.0 32.0 40.0 2.0 41.0 25 32.569508240420404 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24175970250884404 1101 2 0.36636522302968544 1101 3 0.44098190848214713 1101 4 0.44707847360898256 1101 5 0.435448442041924 1101 6 0.33768637465814066 1101 7 0.15216201059749324 1101 8 -0.081569429540167 1101 9 0.31631201343274995 1101 10 0.35329863392207983 1101 11 0.4349010409311518 1101 12 0.5098322792186991 1101 13 0.3801908339552895 1101 14 0.1935757097255646 1101 15 0.3322806305717947 1101 16 0.5141612143593477 1101 17 0.4178455863914934 1101 18 0.272679230891427 1101 19 0.12696670984068703 1101 20 -8.904475956299507E-4 1101 21 -0.028829220466917604 1101 22 0.09601703938597694 1101 23 -0.1617014241813166 1101 24 -0.20972458607981537 1101 25 0.018214208900282358 1102 1 0.43361329793597747 1102 2 0.5664650823411961 1102 3 0.4461273685728564 1102 4 0.5366916294583746 1102 5 0.5336920495702344 1102 6 0.6144742498684366 1102 7 0.3453218937178235 1102 8 0.6072726451723085 1102 9 0.7587118835664555 1102 10 0.4082072355965707 1102 11 0.7406006464130854 1102 12 1.046064730051512 1102 13 0.9846665479739585 1102 14 1.0653760304559654 1102 15 0.9513849958502689 1102 16 1.0304909173393852 1102 17 0.97751103310204 1102 18 1.2389317027783235 1102 19 1.127872576452262 1102 20 1.129743240902343 1102 21 1.1315140889592143 1102 22 0.8560487188916923 1102 23 0.9840211046696652 1102 24 1.1028906456626544 1102 25 1.0576416657836845 1103 1 0.34204728357366676 1103 2 0.3445606246054851 1103 3 0.3012074065110326 1103 4 0.5247575567177307 1103 5 0.41666874469053283 1103 6 0.5315319120251019 1103 7 0.47301773019654547 1103 8 0.4935166689417372 1103 9 0.6469326605248185 1103 10 0.424977941904757 1103 11 0.43349062570193553 1103 12 0.38508346686509753 1103 13 0.5990090174395348 1103 14 0.6063647457841483 1103 15 0.7590108596212488 1103 16 0.8478689248108822 1103 17 0.4846229894344276 1103 18 0.8365102406100391 1103 19 0.7412888511556233 1103 20 0.7182108095685109 1103 21 0.9321494566304693 1103 22 1.2147115917407376 1103 23 1.2254574324090513 1103 24 0.9928573529773672 1103 25 1.084672132461968 1104 1 0.21779609550163315 1104 2 0.16297801117234556 1104 3 0.2189734474864693 1104 4 0.2675872439757043 1104 5 0.39913985682889574 1104 6 0.23005684296470008 1104 7 0.37673157515065725 1104 8 0.4089279309954392 1104 9 0.5854544546726146 1104 10 0.5264997714992745 1104 11 0.5723259533582947 1104 12 0.6080056943368817 1104 13 0.6353448173141629 1104 14 0.7114761625894843 1104 15 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2313 16 -0.3818617253919854 2313 17 -0.47790649159428966 2313 18 -0.4624202535293165 2313 19 -0.3017891360257323 2313 20 -0.43769042967394967 2313 21 -0.41287887370530285 2313 22 -0.4169121549555612 2313 23 -0.3713695599675759 2313 24 -0.5235859114746617 2313 25 -0.46838946204034926 2314 1 -0.36944620172595677 2314 2 -0.34766608736806504 2314 3 -0.3113100891688596 2314 4 -0.36587767412512306 2314 5 -0.6120652188543758 2314 6 -0.6356271700098617 2314 7 -0.5499113720022564 2314 8 -0.5213108571941554 2314 9 -0.5969676296181277 2314 10 -0.6105385385751703 2314 11 -0.5548185557505434 2314 12 -0.5657168791438849 2314 13 -0.5813746961098758 2314 14 -0.6146104468936002 2314 15 -0.4641829554681891 2314 16 -0.4849925174138292 2314 17 -0.4673672630440038 2314 18 -0.659242739412349 2314 19 -0.8749840834530858 2314 20 -0.5406421546351794 2314 21 -0.6521742139481574 2314 22 -0.27339685244028544 2314 23 -0.3134941083120779 2314 24 -0.3625927890905558 2314 25 -0.3796977527423451 2315 1 -0.2911178080986687 2315 2 -0.24494030505648112 2315 3 -0.4701466653125408 2315 4 -0.4966797773567748 2315 5 -0.39500821694413446 2315 6 -0.3285797192920441 2315 7 -0.5897937544832033 2315 8 -0.45410418711400524 2315 9 -0.6278243814249791 2315 10 -0.5333166555132749 2315 11 -0.5814486587458703 2315 12 -0.4716204022538335 2315 13 -0.4452972822183128 2315 14 -0.6141095016209448 2315 15 -0.4430324798778855 2315 16 -0.5179195147946345 2315 17 -0.7485526447963053 2315 18 -0.6612895993347578 2315 19 -0.7156477928605156 2315 20 -0.7758464221473389 2315 21 -0.6576587646482537 2315 22 -0.5722113229891335 2315 23 -0.6080905458157702 2315 24 -0.3412650748779882 2315 25 -0.21396979433433927 2316 1 -1.5327270117728133 2316 2 -1.5695768816141964 2316 3 -1.404525345342897 2316 4 -1.6042020865185798 2316 5 -1.8316335146177032 2316 6 -1.821668131612462 2316 7 -1.7731734289395398 2316 8 -1.837351053363708 2316 9 -2.1595773189069867 2316 10 -2.1758964058600156 2316 11 -2.2205047391373647 2316 12 -2.473549719501893 2316 13 -2.293319552126455 2316 14 -2.736896576700108 2316 15 -2.607172955632212 2316 16 -2.512656907828422 2316 17 -2.4945477904273723 2316 18 -2.6933974553000013 2316 19 -2.6411472956475244 2316 20 -2.582481271544143 2316 21 -2.4779529053856955 2316 22 -2.414978182434872 2316 23 -2.143943854899071 2316 24 -2.43059331395893 2316 25 -2.4438104244863865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59360.0 3 2.0 4 1014.0 5 8384.0 6 10296.0 7 8337.0 8 6637.0 9 5960.0 10 5561.0 11 5416.0 12 4941.0 13 4714.0 14 4694.0 15 4550.0 16 4786.0 17 5057.0 18 5488.0 19 6067.0 20 7495.0 21 9574.0 22 12045.0 23 15180.0 24 18149.0 25 21254.0 26 24241.0 27 27847.0 28 32328.0 29 39023.0 30 48675.0 31 61444.0 32 76971.0 33 97229.0 34 123638.0 35 162420.0 36 226451.0 37 341188.0 38 431823.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.00084335818984 22.059595229716194 15.606759776187312 22.33280163590665 2 14.782814785926435 22.55845878026531 41.718791083470464 20.939935350337795 3 19.715190907351214 26.277759136704525 30.903119374724813 23.103930581219444 4 13.308205051344776 19.242531657123415 38.70059676212337 28.74866652940844 5 12.500220470676362 39.010029600133635 36.418228204268935 12.071521724921071 6 30.462873118944277 39.335424706169725 18.038635252165317 12.16306692272068 7 26.90823077429717 32.61494036786934 22.50680543231415 17.970023425519347 8 23.780195297367182 40.097363449240476 20.539258878178483 15.58318237521386 9 25.7435929882136 16.04837367152101 21.14618571660463 37.06184762366076 10 14.396763056861728 31.22673071128631 34.77680930631524 19.59969692553672 11 33.86374821793356 23.042994151658586 25.36490549148731 17.72835213892054 12 22.859977419811546 25.58157987676839 33.0147870673708 18.543655636049266 13 29.060868491924495 23.00083132848158 26.473015015254852 21.46528516433907 14 19.881612186041252 24.040795772723193 28.486821395065654 27.590770646169897 15 22.114530408315566 33.509953900942776 23.97970376078899 20.395811929952668 16 20.454259041539974 29.877364787248883 29.775665775871147 19.892710395339996 17 20.307493002682758 30.343074691467187 29.213287357013318 20.136144948836737 18 19.92024847542239 28.93899563280278 32.23568240244077 18.90507348933405 19 22.679346284355827 27.71497724089182 28.828065400606462 20.77761107414589 20 22.33301992128569 28.962436710387042 29.894738152272616 18.80980521605465 21 21.856782276470916 27.9655167227714 28.6253934289266 21.552307571831086 22 21.629372707428903 29.645391468588695 29.280135916761356 19.445099907221046 23 21.235075112576812 29.085865393242226 29.427472424320843 20.251587069860115 24 22.072626889094142 28.015303082242397 30.383577969328492 19.52849205933497 25 21.71516236893246 28.666325399653235 30.00177894507001 19.61673328634429 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 284.0 1 284.0 2 512.0 3 740.0 4 740.0 5 740.0 6 1984.5 7 3229.0 8 3229.0 9 3229.0 10 4634.0 11 6039.0 12 6039.0 13 6039.0 14 9184.0 15 12329.0 16 12329.0 17 12329.0 18 21459.5 19 30590.0 20 30590.0 21 30590.0 22 48267.0 23 65944.0 24 65944.0 25 65944.0 26 94975.0 27 124006.0 28 124006.0 29 124006.0 30 159293.0 31 194580.0 32 194580.0 33 194580.0 34 209613.0 35 224646.0 36 224646.0 37 224646.0 38 231278.0 39 237910.0 40 237910.0 41 237910.0 42 243588.0 43 249266.0 44 249266.0 45 249266.0 46 244750.5 47 240235.0 48 240235.0 49 240235.0 50 221734.5 51 203234.0 52 203234.0 53 203234.0 54 172174.5 55 141115.0 56 141115.0 57 141115.0 58 117107.0 59 93099.0 60 93099.0 61 93099.0 62 73917.0 63 54735.0 64 54735.0 65 54735.0 66 41219.0 67 27703.0 68 27703.0 69 27703.0 70 19873.0 71 12043.0 72 12043.0 73 12043.0 74 8250.0 75 4457.0 76 4457.0 77 4457.0 78 2976.5 79 1496.0 80 1496.0 81 1496.0 82 948.5 83 401.0 84 401.0 85 401.0 86 265.5 87 130.0 88 130.0 89 130.0 90 78.5 91 27.0 92 27.0 93 27.0 94 14.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38102123232649066 2 0.0 3 0.0 4 0.0 5 0.028264131158015165 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.00674190284503114 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1928239.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.58097841507248 #Duplication Level Percentage of deduplicated Percentage of total 1 90.75279922891188 69.49938158856706 2 6.826418216425793 10.455475721687224 3 1.197239239818429 2.7505725714663867 4 0.4058024650539573 1.2430699926832123 5 0.208345534763852 0.797765245031364 6 0.12227492615030082 0.561836008813246 7 0.0841308594572837 0.45099764724978164 8 0.05985371436296404 0.366692480615365 9 0.0424669070815482 0.29269415651102376 >10 0.2545321697074129 3.5147555054355286 >50 0.02233621385922686 1.2028837109474337 >100 0.01867786071771731 2.871875622650148 >500 0.0028309457232413415 1.5807052565275161 >1k 0.0019547006184285403 2.7886752272441924 >5k 3.370173480049208E-4 1.6226192645704987 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8789 0.45580449311522064 No Hit TATCAACGCAGAGTACTTTTTTTTT 5914 0.30670471865780125 No Hit GGTATCAACGCAGAGTACTTTTTTT 5732 0.29726605467475764 No Hit GTCCTACAGTGGACATTTCTAAATT 5686 0.29488045828343895 No Hit CTGTAGGACGTGGAATATGGCAAGA 5314 0.27558824398842674 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4269 0.2213937172725995 No Hit CTTTAGGACGTGAAATATGGCGAGG 4052 0.21013992560050906 No Hit TTGTAGAACAGTGTATATCAATGAG 3191 0.16548778444995668 No Hit GATATACACTGTTCTACAAATCCCG 2859 0.14827000179956945 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2790 0.1446916072125914 No Hit CTGAAGGACCTGGAATATGGCGAGA 2321 0.12036889617936365 No Hit GTCCTACAGTGTGCATTTCTCATTT 2220 0.11513095627668563 No Hit GTGTATATCAATGAGTTACAATGAA 2116 0.1097374340006607 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 5.186079111562415E-5 0.0 0.0 0.0 6 0.0 5.186079111562415E-5 0.0 0.0 5.186079111562415E-5 7 0.0 5.186079111562415E-5 0.0 0.0 5.186079111562415E-5 8 0.0 5.186079111562415E-5 0.0 5.186079111562415E-5 5.186079111562415E-5 9 0.0 5.186079111562415E-5 0.0 5.186079111562415E-5 5.186079111562415E-5 10 0.0 5.186079111562415E-5 0.0 5.186079111562415E-5 5.186079111562415E-5 11 0.0 5.186079111562415E-5 0.0 1.5558237334687246E-4 5.186079111562415E-5 12 0.0 5.186079111562415E-5 0.0 1.5558237334687246E-4 5.186079111562415E-5 13 0.0 5.186079111562415E-5 0.0 1.5558237334687246E-4 5.186079111562415E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 440 0.0 15.541338 18 CCAACGA 300 0.0 14.880165 19 CCAACGT 175 0.0 13.56854 19 GGCGTCA 50 0.0015027715 13.296479 12 TAGGACC 780 0.0 12.423654 4 CCGTGCC 55 0.003074999 12.0877075 9 GGTATCA 1700 0.0 11.439697 1 AGGACCG 75 2.073414E-4 11.400529 5 GATATAC 1345 0.0 11.26959 1 AATCCCG 750 0.0 11.144294 19 CTACGGT 60 0.005874497 11.083848 4 ACTGTTC 1270 0.0 10.918368 8 ACTGTCC 280 0.0 10.854268 8 TGTAGGA 2995 0.0 10.689979 2 AACTGTC 160 5.475158E-10 10.68467 7 GGCGAGG 1195 0.0 10.650452 19 CTGTAGG 2800 0.0 10.554484 1 CTAGACA 190 9.094947E-12 10.500487 4 GTCCTAC 2620 0.0 10.479113 1 TATACTG 345 0.0 10.464253 5 >>END_MODULE