FastQCFastQC Report
Thu 26 May 2016
SRR1512160_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512160_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671518
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT61100.36553599781755264No Hit
GTATCAACGCAGAGTACTTTTTTTT54370.3252731947846209No Hit
CTGTAGGACGTGGAATATGGCAAGA52400.3134875005833021No Hit
GTCCTAAAGTGTGTATTTCTCATTT45470.2720281803725715No Hit
CTTTAGGACGTGAAATATGGCGAGG41410.24773888166325458No Hit
GGTATCAACGCAGAGTACTTTTTTT32980.19730568261903253No Hit
TATCAACGCAGAGTACTTTTTTTTT32490.19437421553342532No Hit
GTCCTACAGTGTGCATTTCTCATTT22720.13592435139795084No Hit
CTGAAGGACCTGGAATATGGCGAGA21150.1265316915522298No Hit
TTGTAGAACAGTGTATATCAATGAG18960.11342982845533223No Hit
GATATACACTGTTCTACAAATCCCG18520.11079749066417471No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG454.2640953E-415.7029911
AAGCGTG400.00552786514.15150457
CCAACGA1850.013.770678519
TCCAACG2750.013.38120518
ACGGCAA500.001579359813.20886117
TAGGACC6950.013.0316024
CCACCTT6650.012.76903813
CCAACGT854.255984E-612.21060319
CAAGTCG1551.8189894E-1212.1733376
GGTATCA10250.011.9167641
GGCGAGG13200.011.86581319
CACCTTT7750.011.80884414
CGTAGGA600.003735359511.7772422
TCCACCT7500.011.57347912
AAGGCGT908.060073E-611.5308566
GATATAC9550.011.4160851
ACCTTTT8100.011.41506715
TGTAGGA26550.011.2924652
GACGTGG14900.011.2072327
AGGACGT31000.011.19947055
Produced by FastQC (version 0.11.5)