FastQCFastQC Report
Thu 26 May 2016
SRR1512160_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512160_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671518
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71440.4273959359097539No Hit
CTGTAGGACGTGGAATATGGCAAGA52930.31665827110446915No Hit
TATCAACGCAGAGTACTTTTTTTTT50610.3027786718420023No Hit
GGTATCAACGCAGAGTACTTTTTTT50140.299966856474175No Hit
GTCCTACAGTGGACATTTCTAAATT45150.27011375288809336No Hit
CTTTAGGACGTGAAATATGGCGAGG42920.256772586355636No Hit
GTCCTAAAGTGTGTATTTCTCATTT34970.20921102853813123No Hit
CTGAAGGACCTGGAATATGGCGAGA25550.15285506946380475No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25500.15255594016935506No Hit
TTGTAGAACAGTGTATATCAATGAG23110.13825755989465863No Hit
GATATACACTGTTCTACAAATCCCG20740.12407883133774211No Hit
TTTCTAAATTTTCCACCTTTTTCAG18650.11157522682974398No Hit
CTGTAGGACCTGGAATATGGCGAGA17690.10583194437630945No Hit
GTCCTACAGTGTGCATTTCTCATTT17120.10242187041958267No Hit
GAATATGGCAAGAAAACTGAAAATC16760.10026813949954472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8500.013.7473184
GGACGTA751.4825524E-512.6634966
CCACCTT5350.012.42679513
GGTATCA16400.012.3223831
ACCTTTT6350.012.11507715
TCCAACG2750.012.08788318
CACCTTT5850.011.85173314
TAGGACT3100.011.3389234
TAGACTG1850.011.297515
AGGACCT18500.011.1434545
TGTAGGA29200.011.1269232
TTCCACC6150.011.11916711
ATAGGAC2400.011.0835433
TGTAGAA10250.011.0294762
AGAACAG7150.011.028185
ACTGTTC8800.011.0086078
GTCCTAG1855.456968E-1210.8205731
TCCACCT6700.010.77342112
GTAGGAC30350.010.7679143
GAACAGT6400.010.6848256
Produced by FastQC (version 0.11.5)