##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512160_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1671518 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.74865062775274 31.0 28.0 31.0 23.0 34.0 2 28.973351169416063 31.0 28.0 33.0 24.0 34.0 3 28.990382394924854 31.0 28.0 34.0 24.0 34.0 4 32.54338032853968 35.0 32.0 37.0 26.0 37.0 5 31.975987096758754 35.0 32.0 37.0 22.0 37.0 6 31.902765629804765 35.0 32.0 37.0 22.0 37.0 7 31.621822199940414 35.0 32.0 37.0 18.0 37.0 8 31.458059081625205 35.0 32.0 37.0 17.0 37.0 9 32.626434773660826 37.0 32.0 39.0 17.0 39.0 10 32.29301568992975 37.0 32.0 39.0 17.0 39.0 11 32.32422684051264 37.0 32.0 39.0 16.0 39.0 12 32.02976396305633 37.0 32.0 39.0 11.0 39.0 13 32.005415436746716 37.0 32.0 39.0 11.0 39.0 14 32.56902528121145 37.0 31.0 40.0 10.0 41.0 15 32.49365606592331 37.0 31.0 40.0 10.0 41.0 16 32.327499314993915 37.0 31.0 40.0 10.0 41.0 17 32.22158002486363 37.0 31.0 40.0 10.0 41.0 18 32.10013951390293 37.0 31.0 40.0 8.0 41.0 19 32.13248436451178 37.0 31.0 40.0 2.0 41.0 20 31.943242609412522 37.0 31.0 40.0 2.0 41.0 21 31.758964007566775 37.0 31.0 40.0 2.0 41.0 22 31.835154033638883 37.0 31.0 40.0 2.0 41.0 23 31.65102559469895 37.0 31.0 40.0 2.0 41.0 24 31.418915620412104 37.0 31.0 40.0 2.0 41.0 25 30.85113172577262 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3255693603097711 1101 2 0.41017242430889667 1101 3 0.5304100757074934 1101 4 0.6967900829305691 1101 5 0.6878554453126462 1101 6 0.7380709696204235 1101 7 0.459523845206256 1101 8 0.4371712235061196 1101 9 0.49468140947359984 1101 10 0.6559164586047785 1101 11 0.538016099722519 1101 12 0.6716881561040431 1101 13 0.4732203600578977 1101 14 0.6038370297418822 1101 15 0.4345422348665622 1101 16 0.17935786714831892 1101 17 0.2618446401661103 1101 18 0.7068978215998172 1101 19 0.616455992407893 1101 20 0.37339260035038535 1101 21 0.35051751237825357 1101 22 0.214468620327807 1101 23 0.23130961116146054 1101 24 0.11285566982466477 1101 25 0.3467752241708588 1102 1 0.3863084369122163 1102 2 0.5951862156994068 1102 3 0.42896818909756007 1102 4 0.5412676441421169 1102 5 0.5143603129171659 1102 6 0.8781110085388519 1102 7 0.747065466819798 1102 8 0.8593698531442548 1102 9 0.997732883014109 1102 10 0.9035526871030015 1102 11 0.8582406089936541 1102 12 1.1667972079996325 1102 13 1.2445185809031578 1102 14 1.1832995296718565 1102 15 1.3268143874626546 1102 16 1.075214660785413 1102 17 1.17182150200383 1102 18 1.3144155767312 1102 19 1.211013388683007 1102 20 1.2623415600668224 1102 21 1.4384294566375004 1102 22 1.253244183888654 1102 23 1.1779891261377706 1102 24 1.2860529380648735 1102 25 1.167545039607134 1103 1 0.47206842952131467 1103 2 0.3834374771975497 1103 3 0.4733142097410301 1103 4 0.5357834245691109 1103 5 0.4789623894202997 1103 6 0.7871263288578731 1103 7 0.666600058794593 1103 8 0.6207932339228073 1103 9 0.8948109238901765 1103 10 0.7654406736048855 1103 11 0.675715716362312 1103 12 0.7834330589805667 1103 13 0.6844346795765652 1103 14 0.7500723189958904 1103 15 0.7989173787114865 1103 16 1.059250676846041 1103 17 0.6240632671652051 1103 18 1.047642908968058 1103 19 1.0711077442461772 1103 20 0.49247212861926926 1103 21 0.8331996640082266 1103 22 0.8442452742898467 1103 23 1.0183006623878335 1103 24 0.9017558092115472 1103 25 0.8635321930522295 1104 1 0.4333411323911385 1104 2 0.430715649367869 1104 3 0.6030525125801773 1104 4 0.368444280004141 1104 5 0.6725593292963374 1104 6 0.5221432736232074 1104 7 0.6280081759843057 1104 8 0.5735534091705041 1104 9 1.0902677135402072 1104 10 0.8449881980393812 1104 11 1.049200993350503 1104 12 1.0368597602877365 1104 13 1.020914098996652 1104 14 1.2092670711517357 1104 15 1.2985141316438629 1104 16 1.3825889154532618 1104 17 1.424673470454458 1104 18 1.4471409172990306 1104 19 1.5379917528935252 1104 20 1.0716259928406444 1104 21 1.2858999214152718 1104 22 1.3165937900050935 1104 23 1.1250155349545743 1104 24 1.4109531977523808 1104 25 1.3390210027963079 1105 1 -0.1528442676344497 1105 2 -0.026511497286051622 1105 3 0.0767499521170869 1105 4 0.01711836469953454 1105 5 -0.07403400196101018 1105 6 0.09137378289152309 1105 7 -0.02908240217777447 1105 8 -0.14940777954868167 1105 9 -0.038632907083027135 1105 10 0.17193576249753306 1105 11 0.11668169995817834 1105 12 0.09080672850342708 1105 13 0.15958150915595226 1105 14 0.27996827160265525 1105 15 0.16793914389459275 1105 16 0.00935440870510007 1105 17 -0.298674853428313 1105 18 -0.14724070443219617 1105 19 0.13421943302754613 1105 20 0.2333163281606474 1105 21 0.2883401100885976 1105 22 0.22050551473901692 1105 23 0.037895941268466515 1105 24 0.1665957757406531 1105 25 0.16479026420397247 1106 1 -0.31160489203641006 1106 2 -0.4492792153791747 1106 3 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0.43958975967346703 1107 15 0.39629667796551615 1107 16 0.42567059568241206 1107 17 0.4146094464927259 1107 18 0.4584305324282667 1107 19 0.25719930490996035 1107 20 0.35206160780509776 1107 21 0.1696626256400755 1107 22 0.16739457418957926 1107 23 -0.009448112908792439 1107 24 0.13721683495316128 1107 25 0.06153940786781931 1108 1 0.21033796316621078 1108 2 0.319046110438979 1108 3 0.15285401005112575 1108 4 0.3070113375905592 1108 5 0.3853866576543261 1108 6 0.14947698667934617 1108 7 0.33134973664502 1108 8 0.3763793013973853 1108 9 0.30048809881280647 1108 10 0.20844681555628597 1108 11 0.38439460329729513 1108 12 0.24476103722736653 1108 13 0.19524581120571938 1108 14 0.33241044571906997 1108 15 0.2535820922890011 1108 16 0.5147713966009988 1108 17 0.7016496112050419 1108 18 0.49253422249393 1108 19 0.567022899899797 1108 20 0.439056486528969 1108 21 0.3258578354713393 1108 22 0.5391399531009071 1108 23 0.5384764648859779 1108 24 0.3631945359344826 1108 25 0.34699518164574883 1109 1 -0.31444844522170357 1109 2 -0.051939288961346364 1109 3 -0.2364689800935018 1109 4 -0.05393121181484162 1109 5 -0.204921869833246 1109 6 0.05730372572827136 1109 7 -0.31096942998089006 1109 8 -0.14445379680647363 1109 9 -0.03282579715087053 1109 10 0.1120004683396516 1109 11 0.014866379609536295 1109 12 -0.159211459521579 1109 13 0.03533833308529566 1109 14 -0.1154378374595808 1109 15 -0.09419257968971095 1109 16 0.10482723828299356 1109 17 0.0945026784846803 1109 18 0.1876465391399691 1109 19 0.16747522630213751 1109 20 -0.03758606525907737 1109 21 -0.047073808942670325 1109 22 -0.051540392457905426 1109 23 -0.04998237742428557 1109 24 -0.20405876308876003 1109 25 -0.2796737673443239 1110 1 -0.5083241680942265 1110 2 -0.5701046804034569 1110 3 -0.5194933290123451 1110 4 -0.5099401236701411 1110 5 -0.542815426654002 1110 6 -0.7075987416680825 1110 7 -0.8349459550995526 1110 8 -0.8157952204254499 1110 9 -0.9951161337256913 1110 10 -0.8496212231468654 1110 11 -0.7056495712549662 1110 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-0.4371115400703651 2315 9 -0.6660276961640292 2315 10 -0.21148853878927554 2315 11 -0.23825548658117413 2315 12 -0.5069965463429007 2315 13 -0.6099375384800076 2315 14 -0.8609237470099664 2315 15 -0.6898040222636723 2315 16 -0.7955480360068634 2315 17 -0.6709569120857743 2315 18 -0.7581121146899719 2315 19 -0.8644784882629821 2315 20 -0.7680674263951452 2315 21 -0.5772238142819397 2315 22 -0.6216494018510019 2315 23 -0.7971306862411431 2315 24 -0.6795210112499213 2315 25 -0.8276316377068866 2316 1 -1.523819525145612 2316 2 -1.713709234602959 2316 3 -1.4252517467764605 2316 4 -1.713156236697202 2316 5 -1.856565136283578 2316 6 -1.8237379765196806 2316 7 -1.852123971527071 2316 8 -1.5415269538922054 2316 9 -1.956143955679483 2316 10 -1.8694371431892733 2316 11 -1.763823424217339 2316 12 -1.893899973588212 2316 13 -1.8743681433957171 2316 14 -2.0477003949540276 2316 15 -1.9062402065897501 2316 16 -2.0355505024575287 2316 17 -1.9312417310819292 2316 18 -1.9654999752682443 2316 19 -2.0322601961679574 2316 20 -1.824733663301469 2316 21 -2.0521393570318835 2316 22 -1.970721306361991 2316 23 -1.954833112705412 2316 24 -1.8360574792178284 2316 25 -1.9663157675916736 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72691.0 3 2.0 4 1199.0 5 9561.0 6 11395.0 7 8998.0 8 7503.0 9 6647.0 10 6184.0 11 5913.0 12 5504.0 13 5297.0 14 5136.0 15 5080.0 16 5144.0 17 5588.0 18 5871.0 19 6466.0 20 8125.0 21 10398.0 22 12897.0 23 16018.0 24 19154.0 25 22037.0 26 25273.0 27 28779.0 28 32950.0 29 39617.0 30 49344.0 31 61374.0 32 76790.0 33 96538.0 34 121155.0 35 157663.0 36 211862.0 37 281706.0 38 225659.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.48548733132501 23.409412834220102 16.18187025754065 21.92322957691423 2 15.1562830911782 23.780719082893516 41.05172663411342 20.011271191814863 3 20.00672442653923 27.020588471078383 31.340613741521178 21.632073360861206 4 14.0176773447848 20.497475947013434 38.500811836905136 26.98403487129663 5 12.297308376115227 39.63333738262781 36.44753999376448 11.621814247492479 6 29.96491811634694 40.26453798283955 17.96403030060101 11.8065136002125 7 26.60850795504446 32.98091914056564 23.00615368784542 17.404419216544483 8 23.39597898437229 40.78759546711432 20.7029179464415 15.113507602071888 9 25.57423850655512 16.02854411379357 21.582597375559224 36.81462000409209 10 14.53325659669833 31.714525359583323 34.87542461403347 18.876793429684874 11 33.3559674499467 23.136155279213266 26.061938908225933 17.445938362614104 12 22.921260794080588 26.032684063228757 33.169849202940085 17.876205939750577 13 28.957929259511417 23.395919158513397 26.618259570043517 21.02789201193167 14 19.601344406700974 24.999491480199435 28.67860232435427 26.72056178874532 15 21.735272967446356 34.51204234713596 23.96258969391894 19.790094991498748 16 20.13391420253925 30.4124753667026 30.10006473157932 19.35354569917883 17 19.99457977718457 31.095985804520204 29.286792005829433 19.622642412465794 18 19.63795783234162 29.1947798348567 32.85319093183561 18.314071400966068 19 22.67687216051517 27.977323606446358 29.28200593711824 20.063798295920236 20 22.35423130352171 29.4315107584842 30.04801623434507 18.166241703649018 21 21.476705605324025 28.315279883315647 28.915213596263996 21.29280091509634 22 21.60479276920739 30.251424154570877 29.542128771571708 18.601654304650026 23 20.767829003337088 29.874940024576464 29.764022882194507 19.59320808989194 24 22.04893994560633 28.46065672041821 30.646514126680056 18.843889207295405 25 21.498089336601634 29.40734434240811 30.211396492420466 18.883169828569788 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 263.0 1 263.0 2 481.0 3 699.0 4 699.0 5 699.0 6 1694.5 7 2690.0 8 2690.0 9 2690.0 10 4116.5 11 5543.0 12 5543.0 13 5543.0 14 8753.0 15 11963.0 16 11963.0 17 11963.0 18 20364.0 19 28765.0 20 28765.0 21 28765.0 22 45874.5 23 62984.0 24 62984.0 25 62984.0 26 89532.0 27 116080.0 28 116080.0 29 116080.0 30 147870.0 31 179660.0 32 179660.0 33 179660.0 34 193386.0 35 207112.0 36 207112.0 37 207112.0 38 211029.5 39 214947.0 40 214947.0 41 214947.0 42 216681.5 43 218416.0 44 218416.0 45 218416.0 46 211416.5 47 204417.0 48 204417.0 49 204417.0 50 185873.5 51 167330.0 52 167330.0 53 167330.0 54 138799.0 55 110268.0 56 110268.0 57 110268.0 58 89863.5 59 69459.0 60 69459.0 61 69459.0 62 54305.5 63 39152.0 64 39152.0 65 39152.0 66 29232.5 67 19313.0 68 19313.0 69 19313.0 70 13694.0 71 8075.0 72 8075.0 73 8075.0 74 5569.0 75 3063.0 76 3063.0 77 3063.0 78 2007.5 79 952.0 80 952.0 81 952.0 82 624.0 83 296.0 84 296.0 85 296.0 86 176.0 87 56.0 88 56.0 89 56.0 90 34.0 91 12.0 92 12.0 93 12.0 94 7.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3655958236764426 2 0.0 3 0.0 4 0.0 5 0.0266225072060247 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.006521018619003803 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1671518.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.29486929717906 #Duplication Level Percentage of deduplicated Percentage of total 1 91.75060726932458 70.9185119681925 2 6.101236762834536 9.431885962688787 3 1.0221349117975924 2.370173532344357 4 0.3587791142293417 1.1092713896365858 5 0.18900304943236482 0.7304483001321456 6 0.11201664654569454 0.5194987232314657 7 0.0750323468653733 0.40597308108137425 8 0.05151625801902841 0.3185553944208443 9 0.04431199877845559 0.30825811384897345 >10 0.24653779879553764 3.5179946829550155 >50 0.02411587175757857 1.3194944961860038 >100 0.019320416320579133 3.0742178535829106 >500 0.0028477078011262136 1.5406139458282406 >1k 0.0022319871954773027 3.0958624451056735 >5k 3.0786030282445553E-4 1.3392401107651601 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7144 0.4273959359097539 No Hit CTGTAGGACGTGGAATATGGCAAGA 5293 0.31665827110446915 No Hit TATCAACGCAGAGTACTTTTTTTTT 5061 0.3027786718420023 No Hit GGTATCAACGCAGAGTACTTTTTTT 5014 0.299966856474175 No Hit GTCCTACAGTGGACATTTCTAAATT 4515 0.27011375288809336 No Hit CTTTAGGACGTGAAATATGGCGAGG 4292 0.256772586355636 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3497 0.20921102853813123 No Hit CTGAAGGACCTGGAATATGGCGAGA 2555 0.15285506946380475 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2550 0.15255594016935506 No Hit TTGTAGAACAGTGTATATCAATGAG 2311 0.13825755989465863 No Hit GATATACACTGTTCTACAAATCCCG 2074 0.12407883133774211 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1865 0.11157522682974398 No Hit CTGTAGGACCTGGAATATGGCGAGA 1769 0.10583194437630945 No Hit GTCCTACAGTGTGCATTTCTCATTT 1712 0.10242187041958267 No Hit GAATATGGCAAGAAAACTGAAAATC 1676 0.10026813949954472 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.196517177798863E-4 0.0 10 0.0 0.0 0.0 1.196517177798863E-4 0.0 11 0.0 0.0 0.0 1.196517177798863E-4 0.0 12 0.0 0.0 0.0 1.196517177798863E-4 0.0 13 0.0 0.0 0.0 1.196517177798863E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 850 0.0 13.747318 4 GGACGTA 75 1.4825524E-5 12.663496 6 CCACCTT 535 0.0 12.426795 13 GGTATCA 1640 0.0 12.322383 1 ACCTTTT 635 0.0 12.115077 15 TCCAACG 275 0.0 12.087883 18 CACCTTT 585 0.0 11.851733 14 TAGGACT 310 0.0 11.338923 4 TAGACTG 185 0.0 11.29751 5 AGGACCT 1850 0.0 11.143454 5 TGTAGGA 2920 0.0 11.126923 2 TTCCACC 615 0.0 11.119167 11 ATAGGAC 240 0.0 11.083543 3 TGTAGAA 1025 0.0 11.029476 2 AGAACAG 715 0.0 11.02818 5 ACTGTTC 880 0.0 11.008607 8 GTCCTAG 185 5.456968E-12 10.820573 1 TCCACCT 670 0.0 10.773421 12 GTAGGAC 3035 0.0 10.767914 3 GAACAGT 640 0.0 10.684825 6 >>END_MODULE