FastQCFastQC Report
Thu 26 May 2016
SRR1512159_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512159_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629506
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT37790.6003119906720508No Hit
CTGTAGGACGTGGAATATGGCAAGA35250.5599628915371736No Hit
GTCCTAAAGTGTGTATTTCTCATTT31980.5080173977690443No Hit
GTATCAACGCAGAGTACTTTTTTTT23600.37489714156815024No Hit
CTTTAGGACGTGAAATATGGCGAGG23490.3731497396371123No Hit
GTCCTACAGTGTGCATTTCTCATTT18690.2968994735554546No Hit
GGTATCAACGCAGAGTACTTTTTTT15070.23939406455220444No Hit
TATCAACGCAGAGTACTTTTTTTTT15030.2387586456681906No Hit
CTGTAGGACCTGGAATATGGCGAGA12840.20396946176843428No Hit
GTCCTTCAGTGTGCATTTCTCATTT11410.18125323666494045No Hit
CTGAAGGACCTGGAATATGGCGAGA11070.17585217615082302No Hit
ATTTAGAAATGTCCACTGTAGGACG10130.1609198323764984No Hit
TTTCTAAATTTTCCACCTTTTTCAG8990.1428103941821047No Hit
GAATATGGCAAGAAAACTGAAAATC8820.14010986392504599No Hit
GGAATATGGCGAGAAAACTGAAAAT7880.12517752015072137No Hit
ATTCCAGGTCCTTCAGTGTGCATTT7610.12088844268362811No Hit
TTCCAGGTCCTTCAGTGTGCATTTC7450.11834676714757286No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7350.11675821993753834No Hit
GACCTGGAATATGGCGAGAAAACTG7080.11246914247044508No Hit
CTGTAGGACATGGAATATGGCAAGA6650.10563838946729659No Hit
ACCTGGAATATGGCGAGAAAACTGA6630.10532068002528966No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6620.10516182530428621No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCA250.006275404718.8682179
CGGTAGG452.104649E-517.938791
GGCCTAC651.7969669E-615.5239521
CTATAGG806.13436E-815.1358541
GTGTAGG601.4498584E-515.1358541
GTACTAA601.4498584E-515.1358541
TCCAACG953.3651304E-1014.89832818
CCAACGA704.7778667E-714.82738419
GGACGTA457.1160536E-414.6764466
GTTGGAC457.1160536E-414.6764463
GGTATCA4100.014.0283521
TAGGACC4200.013.9276484
CACCTTT3300.013.43859414
CCACCTT3200.013.26882413
GGCAAGG1001.1030352E-813.20985219
CGTTTCC500.001576563913.20985214
ACCTTTT3450.013.12780315
CGGTTTC802.152201E-612.97396113
GCCGGTT802.1560427E-612.971911
TCCTATA1101.636181E-812.8425442
Produced by FastQC (version 0.11.5)