##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512159_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 629506 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.221294157641072 31.0 31.0 34.0 26.0 34.0 2 27.559983542650905 31.0 28.0 34.0 10.0 34.0 3 29.12097422423297 31.0 30.0 34.0 16.0 34.0 4 33.6173856960855 37.0 33.0 37.0 28.0 37.0 5 33.993825316994595 37.0 35.0 37.0 30.0 37.0 6 34.2048669909421 37.0 35.0 37.0 31.0 37.0 7 34.22487315450528 37.0 35.0 37.0 31.0 37.0 8 34.225530813050234 37.0 35.0 37.0 31.0 37.0 9 35.85074328123957 39.0 37.0 39.0 31.0 39.0 10 35.72589459036133 39.0 37.0 39.0 31.0 39.0 11 35.741154174860924 39.0 37.0 39.0 31.0 39.0 12 35.66019704339593 39.0 37.0 39.0 30.0 39.0 13 35.68030805107497 39.0 37.0 39.0 30.0 39.0 14 36.871030617658924 40.0 38.0 41.0 31.0 41.0 15 36.835972969280675 40.0 38.0 41.0 31.0 41.0 16 36.799320419503545 40.0 38.0 41.0 31.0 41.0 17 36.80118378538092 40.0 38.0 41.0 31.0 41.0 18 36.77557799290237 40.0 38.0 41.0 31.0 41.0 19 36.791260130959834 40.0 38.0 41.0 31.0 41.0 20 36.743408323351964 40.0 38.0 41.0 31.0 41.0 21 36.66783001274015 40.0 37.0 41.0 30.0 41.0 22 36.592995142222634 40.0 37.0 41.0 30.0 41.0 23 36.48932813984299 40.0 37.0 41.0 30.0 41.0 24 36.43744459941605 40.0 37.0 41.0 30.0 41.0 25 36.33905316232093 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2919029466016383 1101 2 3.353594340927849 1101 3 1.632369191550655 1101 4 0.5483475846940493 1101 5 0.28317858699749365 1101 6 -0.019303047529803052 1101 7 0.17414115139610686 1101 8 0.1486155133172531 1101 9 -0.055576368087365324 1101 10 -0.1788999453114144 1101 11 -0.13545205470772714 1101 12 -0.2688701240386209 1101 13 -0.30434846759817447 1101 14 -0.09624105571803909 1101 15 0.004307889028432044 1101 16 0.06990763090524155 1101 17 -0.2681135259381975 1101 18 -0.2491738948567388 1101 19 -0.35505766080645884 1101 20 -0.15986220442792387 1101 21 -0.029447740812813095 1101 22 0.04042517910656329 1101 23 -0.035770466002048806 1101 24 -0.22137708155202773 1101 25 -0.05228994415028154 1102 1 1.4560738727040388 1102 2 4.333831281064729 1102 3 2.7878270447950477 1102 4 1.800292486366061 1102 5 1.3070805189042787 1102 6 1.256344531089276 1102 7 1.2129958685406947 1102 8 0.9768122559318257 1102 9 0.9588609532329144 1102 10 1.0001024804011394 1102 11 1.1054623522921858 1102 12 1.1867674918324695 1102 13 1.0422685297162175 1102 14 1.233320150207632 1102 15 1.3339319035827089 1102 16 1.2773147174512047 1102 17 1.396832761855272 1102 18 1.267015570620174 1102 19 1.2523191043325923 1102 20 1.1752911352584618 1102 21 1.1378809432381232 1102 22 0.9765228140368265 1102 23 1.062063719218429 1102 24 0.9379619117772648 1102 25 1.214666219758577 1103 1 1.2157684316602904 1103 2 4.146802235439363 1103 3 2.673284509642958 1103 4 1.3918787089341365 1103 5 1.142558670908791 1103 6 0.8072574456631045 1103 7 0.595281660001298 1103 8 0.8394173799154245 1103 9 0.9406090929966311 1103 10 1.0586567804356548 1103 11 0.9037529285956438 1103 12 1.0358670109900672 1103 13 1.09816887121508 1103 14 1.1680902226448353 1103 15 1.0615088480146468 1103 16 1.1604376420363351 1103 17 1.1096307158633252 1103 18 1.006359541225116 1103 19 1.059990716623119 1103 20 1.0919808472032102 1103 21 1.1898925280458243 1103 22 1.0967977281261696 1103 23 1.2495193704565537 1103 24 1.1551320373793885 1103 25 1.1197003803638026 1104 1 1.3296185591624514 1104 2 4.323087927836294 1104 3 2.8419003724440657 1104 4 1.766481153911208 1104 5 1.3079021606374752 1104 6 1.2189985889142463 1104 7 1.0113370824000896 1104 8 1.040621262985546 1104 9 1.249753132133776 1104 10 1.2724379582710768 1104 11 1.0250189758097434 1104 12 1.2434142634005667 1104 13 1.284063739389886 1104 14 1.2625271884217213 1104 15 1.2151659164499264 1104 16 1.4435033849963546 1104 17 1.523582626206867 1104 18 1.1724647606054432 1104 19 1.3669458774509522 1104 20 1.5462081184380665 1104 21 1.4160299239372947 1104 22 1.336897978752738 1104 23 1.5152688971116177 1104 24 1.5769201320702635 1104 25 1.5536476940628958 1105 1 0.9150822183103209 1105 2 3.655828871103555 1105 3 2.265520027642669 1105 4 1.4633293349699201 1105 5 0.8459746144994043 1105 6 0.7221959763221619 1105 7 0.5421489605803842 1105 8 0.5028829778788335 1105 9 0.7821750961346865 1105 10 0.8368945543066175 1105 11 0.6415299502154568 1105 12 0.5883979347000263 1105 13 0.7049037181245978 1105 14 0.824340813393242 1105 15 0.8432816603551458 1105 16 0.6979273236308146 1105 17 0.6936745226145575 1105 18 0.8267216148623007 1105 19 0.7296253032882731 1105 20 0.6043534818465872 1105 21 0.850768284991446 1105 22 0.8226274539558958 1105 23 0.8638220982963958 1105 24 0.8212126816259726 1105 25 0.7825988598561651 1106 1 0.16112579222274803 1106 2 3.1333677256915884 1106 3 1.5176194764634303 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-0.9415599525075251 2315 15 -1.0283467112732225 2315 16 -1.1514455257420337 2315 17 -1.1073022783622477 2315 18 -0.8462165580758736 2315 19 -1.3792658056028344 2315 20 -1.0128626353695296 2315 21 -1.013756079532918 2315 22 -1.0588799275514802 2315 23 -1.0421053076310116 2315 24 -0.980319119905829 2315 25 -1.2038036265463177 2316 1 -1.0320693544652109 2316 2 0.44658885986687125 2316 3 -0.06328021629900604 2316 4 -0.5954519650759451 2316 5 -0.9212747750461574 2316 6 -0.8861719914468011 2316 7 -1.0468141192312785 2316 8 -0.9573720564194588 2316 9 -0.8651731186012199 2316 10 -0.8413473369136426 2316 11 -1.7213260881813497 2316 12 -0.9246815168153617 2316 13 -1.0266740034697577 2316 14 -1.1960869008780648 2316 15 -1.1315294409118621 2316 16 -0.9525157810552187 2316 17 -1.0644798761388472 2316 18 -1.385747742365794 2316 19 -1.5359277792365233 2316 20 -1.540959884764618 2316 21 -1.504193262981751 2316 22 -1.4484047122509764 2316 23 -1.3853153421887967 2316 24 -1.00157185496888 2316 25 -0.8146932915125262 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19285.0 3 1.0 4 169.0 5 852.0 6 945.0 7 781.0 8 732.0 9 738.0 10 693.0 11 672.0 12 604.0 13 610.0 14 630.0 15 669.0 16 626.0 17 721.0 18 862.0 19 1048.0 20 1423.0 21 1727.0 22 2065.0 23 2320.0 24 2730.0 25 3132.0 26 3527.0 27 4106.0 28 4614.0 29 5771.0 30 7298.0 31 9931.0 32 13548.0 33 19553.0 34 29093.0 35 45605.0 36 73799.0 37 102153.0 38 266473.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.948785237948485 19.718636518158682 13.403525939387393 26.929052304505436 2 13.682618962676043 20.19095187129436 45.022849667872855 21.103579498156737 3 18.25526682827487 24.079198609703482 30.309321912737925 27.356212649283723 4 11.332219232223363 16.53725302062252 39.73147197961576 32.39905576753836 5 11.456443624048063 38.2568236045407 37.81346007822006 12.473272693191168 6 29.98705331967181 38.81271792916657 18.435437367455382 12.764791383706244 7 26.67774492780331 31.373031847882555 23.499152449476615 18.45007077483752 8 21.99661321734821 41.52033499283565 21.11274554968499 15.370306240131152 9 25.22454114813838 15.296756504306552 22.149590313674533 37.329112033880534 10 14.53392408642703 28.81764125570512 36.255061725649306 20.393372932218547 11 32.186055378163275 21.61352841188899 28.105609302768908 18.09480690717883 12 23.865062445790826 23.772132434003808 33.936769466851786 18.42603565335358 13 28.706477777813078 22.977541119544533 26.71030935368368 21.605671748958706 14 17.854822439853535 24.770414849762908 28.70350513498701 28.67125757539654 15 19.978205132278326 36.3753482889758 23.450133914529804 20.19631266421607 16 18.30308209929691 28.28233567273386 33.51580445619263 19.8987777717766 17 18.621297686277313 30.877117735363502 31.223898142191086 19.2776864361681 18 18.47014451124188 26.89694835979105 36.02327177518779 18.60963535377928 19 22.075823887781283 26.352176779430803 29.509837406769023 22.062161926018888 20 22.009162740307477 28.089486041435663 31.36920061127297 18.532150606983887 21 19.137741217513977 27.52139522100584 29.10369632528044 24.237167236199745 22 20.879435102741088 31.425190033280646 29.24185259612864 18.453522267849625 23 18.115906343379017 30.43411264021249 31.659706016448208 19.790274999960285 24 21.745941214132632 26.58550418866384 32.87318667316983 18.7953679240337 25 19.529313828908403 28.862015937368934 32.141949341026645 19.466720892696006 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 236.0 1 236.0 2 254.5 3 273.0 4 273.0 5 273.0 6 688.5 7 1104.0 8 1104.0 9 1104.0 10 1516.5 11 1929.0 12 1929.0 13 1929.0 14 2864.5 15 3800.0 16 3800.0 17 3800.0 18 6690.0 19 9580.0 20 9580.0 21 9580.0 22 15464.5 23 21349.0 24 21349.0 25 21349.0 26 31568.0 27 41787.0 28 41787.0 29 41787.0 30 54693.5 31 67600.0 32 67600.0 33 67600.0 34 73791.5 35 79983.0 36 79983.0 37 79983.0 38 81058.5 39 82134.0 40 82134.0 41 82134.0 42 81925.5 43 81717.0 44 81717.0 45 81717.0 46 80482.0 47 79247.0 48 79247.0 49 79247.0 50 70865.5 51 62484.0 52 62484.0 53 62484.0 54 51537.0 55 40590.0 56 40590.0 57 40590.0 58 33526.5 59 26463.0 60 26463.0 61 26463.0 62 20963.5 63 15464.0 64 15464.0 65 15464.0 66 11755.0 67 8046.0 68 8046.0 69 8046.0 70 5826.0 71 3606.0 72 3606.0 73 3606.0 74 2520.0 75 1434.0 76 1434.0 77 1434.0 78 970.0 79 506.0 80 506.0 81 506.0 82 313.0 83 120.0 84 120.0 85 120.0 86 81.5 87 43.0 88 43.0 89 43.0 90 25.5 91 8.0 92 8.0 93 8.0 94 5.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 6.493345575737165 3 0.0 4 0.0 5 0.0 6 1.588547210034535E-4 7 0.007148462445155407 8 0.0 9 0.0 10 0.004289077467093245 11 0.002541675536055256 12 0.0 13 0.0 14 1.588547210034535E-4 15 0.0 16 0.0 17 1.588547210034535E-4 18 0.01175524935425556 19 0.0033359491410725234 20 0.0 21 0.006830753003148501 22 0.0017474019310379887 23 0.0023828208150518024 24 0.010960975749238292 25 0.006671898282145047 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 629506.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.26606131250641 #Duplication Level Percentage of deduplicated Percentage of total 1 90.51398864988207 66.31603442061768 2 6.5686927317431545 9.625244888538184 3 1.2812363676357452 2.8161342680104053 4 0.5210637827907368 1.527051642306906 5 0.26695255674127316 0.9779281194868233 6 0.16714879758150447 0.7347800431150938 7 0.1185746874182851 0.6081250222949551 8 0.08639349473820729 0.5063768865992957 9 0.06224758344685358 0.4104571738835281 >10 0.3452077104484795 4.700022970156095 >50 0.033804960497449535 1.7465170563367067 >100 0.027618373468460643 3.9304258957991016 >500 0.004418939754912616 2.113008616870071 >1k 0.0026513638529475697 3.9878929959851526 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3779 0.6003119906720508 No Hit CTGTAGGACGTGGAATATGGCAAGA 3525 0.5599628915371736 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3198 0.5080173977690443 No Hit GTATCAACGCAGAGTACTTTTTTTT 2360 0.37489714156815024 No Hit CTTTAGGACGTGAAATATGGCGAGG 2349 0.3731497396371123 No Hit GTCCTACAGTGTGCATTTCTCATTT 1869 0.2968994735554546 No Hit GGTATCAACGCAGAGTACTTTTTTT 1507 0.23939406455220444 No Hit TATCAACGCAGAGTACTTTTTTTTT 1503 0.2387586456681906 No Hit CTGTAGGACCTGGAATATGGCGAGA 1284 0.20396946176843428 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1141 0.18125323666494045 No Hit CTGAAGGACCTGGAATATGGCGAGA 1107 0.17585217615082302 No Hit ATTTAGAAATGTCCACTGTAGGACG 1013 0.1609198323764984 No Hit TTTCTAAATTTTCCACCTTTTTCAG 899 0.1428103941821047 No Hit GAATATGGCAAGAAAACTGAAAATC 882 0.14010986392504599 No Hit GGAATATGGCGAGAAAACTGAAAAT 788 0.12517752015072137 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 761 0.12088844268362811 No Hit TTCCAGGTCCTTCAGTGTGCATTTC 745 0.11834676714757286 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 735 0.11675821993753834 No Hit GACCTGGAATATGGCGAGAAAACTG 708 0.11246914247044508 No Hit CTGTAGGACATGGAATATGGCAAGA 665 0.10563838946729659 No Hit ACCTGGAATATGGCGAGAAAACTGA 663 0.10532068002528966 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 662 0.10516182530428621 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.588547210034535E-4 2 0.0 0.0 0.0 0.0 1.588547210034535E-4 3 0.0 0.0 0.0 0.0 1.588547210034535E-4 4 0.0 0.0 0.0 0.0 1.588547210034535E-4 5 0.0 0.0 0.0 0.0 1.588547210034535E-4 6 0.0 0.0 0.0 0.0 1.588547210034535E-4 7 0.0 0.0 0.0 0.0 1.588547210034535E-4 8 0.0 0.0 0.0 0.0 1.588547210034535E-4 9 0.0 0.0 0.0 1.588547210034535E-4 1.588547210034535E-4 10 0.0 0.0 0.0 1.588547210034535E-4 1.588547210034535E-4 11 0.0 0.0 0.0 1.588547210034535E-4 1.588547210034535E-4 12 0.0 0.0 0.0 1.588547210034535E-4 1.588547210034535E-4 13 0.0 0.0 0.0 1.588547210034535E-4 1.588547210034535E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTCCA 25 0.0062754047 18.868217 9 CGGTAGG 45 2.104649E-5 17.93879 1 GGCCTAC 65 1.7969669E-6 15.523952 1 CTATAGG 80 6.13436E-8 15.135854 1 GTGTAGG 60 1.4498584E-5 15.135854 1 GTACTAA 60 1.4498584E-5 15.135854 1 TCCAACG 95 3.3651304E-10 14.898328 18 CCAACGA 70 4.7778667E-7 14.827384 19 GGACGTA 45 7.1160536E-4 14.676446 6 GTTGGAC 45 7.1160536E-4 14.676446 3 GGTATCA 410 0.0 14.028352 1 TAGGACC 420 0.0 13.927648 4 CACCTTT 330 0.0 13.438594 14 CCACCTT 320 0.0 13.268824 13 GGCAAGG 100 1.1030352E-8 13.209852 19 CGTTTCC 50 0.0015765639 13.209852 14 ACCTTTT 345 0.0 13.127803 15 CGGTTTC 80 2.152201E-6 12.973961 13 GCCGGTT 80 2.1560427E-6 12.9719 11 TCCTATA 110 1.636181E-8 12.842544 2 >>END_MODULE