FastQCFastQC Report
Thu 26 May 2016
SRR1512159_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512159_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629506
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43070.6841872833618743No Hit
GGTATCAACGCAGAGTACTTTTTTT33690.5351815550606348No Hit
TATCAACGCAGAGTACTTTTTTTTT33140.5264445454054449No Hit
CTGTAGGACGTGGAATATGGCAAGA27380.4349442261074557No Hit
GTCCTACAGTGGACATTTCTAAATT21030.3340714782702627No Hit
CTTTAGGACGTGAAATATGGCGAGG19960.3170740231228932No Hit
GTCCTAAAGTGTGTATTTCTCATTT19180.30468335488462384No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16990.2698941709848675No Hit
CTGAAGGACCTGGAATATGGCGAGA13890.22064920747379693No Hit
CTGTAGGACCTGGAATATGGCGAGA13140.2087351033985379No Hit
TTTCTAAATTTTCCACCTTTTTCAG11880.18871940855210276No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11300.17950583473390244No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11290.179346980012899No Hit
GTCCTACAGTGTGCATTTCTCATTT10520.1671151664956331No Hit
GGAATATGGCGAGAAAACTGAAAAT10300.1636203626335571No Hit
GAATATGGCAAGAAAACTGAAAATC10180.16171410598151567No Hit
ATTTAGAAATGTCCACTGTAGGACG10110.16060212293449147No Hit
ACAGTGGACATTTCTAAATTTTCCA8840.14042757336705292No Hit
ACCTGGAATATGGCGAGAAAACTGA8430.13391452980591131No Hit
GAAATATGGCGAGGAAAACTGAAAA7660.12168271628864538No Hit
ATTCCAGGTCCTTCAGTGTGCATTT7610.12088844268362811No Hit
CTGTAGGACATGGAATATGGCAAGA6950.11040403109740018No Hit
GACCTGGAATATGGCGAGAAAACTG6850.10881548388736566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGAG456.7675003E-414.7740096
TACACGG653.3650103E-614.615145
GGTATCA7250.014.5956061
TAGACAG954.8803486E-913.9997665
AGGACGA500.001498007813.2997785
GACTGTG1001.4418947E-712.346857
TTAGGGC550.003065342812.0907083
TAGGGCG550.003065342812.0907084
TAGGACC4800.012.0727164
AGACTGT802.8674938E-511.8719716
TAGAAAT4300.011.7091064
CTCTAGA658.028954E-411.6893269
AAATGTC3850.011.5944457
ATAATTC752.0704E-411.399813
CCACCTT3700.011.29441613
CACCTTT3750.011.14382414
AGGACCT9050.011.1268865
GTGGTAT1803.6379788E-1211.1220261
TATACAC2050.011.1217653
CAGTCCT600.005880230611.0805079
Produced by FastQC (version 0.11.5)