##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512159_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 629506 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.75754798206848 31.0 28.0 31.0 16.0 34.0 2 27.958681887067 31.0 28.0 31.0 16.0 34.0 3 27.902757082537736 31.0 27.0 31.0 16.0 34.0 4 31.33379824815014 35.0 32.0 37.0 20.0 37.0 5 30.69961366531852 35.0 30.0 37.0 16.0 37.0 6 30.703801075764172 35.0 31.0 37.0 11.0 37.0 7 30.378526971943078 35.0 30.0 37.0 10.0 37.0 8 30.189793266466086 35.0 30.0 37.0 10.0 37.0 9 31.195802740561646 35.0 30.0 39.0 10.0 39.0 10 30.808052663517106 35.0 30.0 39.0 8.0 39.0 11 30.864514397003365 35.0 30.0 39.0 2.0 39.0 12 30.561481542669966 35.0 30.0 39.0 2.0 39.0 13 30.555119411093777 35.0 30.0 39.0 2.0 39.0 14 30.90523998182702 36.0 29.0 39.0 2.0 41.0 15 30.835898307561802 36.0 29.0 39.0 2.0 40.0 16 30.64088348641633 36.0 28.0 39.0 2.0 40.0 17 30.530909951612852 36.0 28.0 39.0 2.0 40.0 18 30.375951936915612 36.0 27.0 39.0 2.0 40.0 19 30.43827699815411 36.0 27.0 39.0 2.0 40.0 20 30.271177717130577 36.0 27.0 39.0 2.0 40.0 21 30.011407357515257 36.0 27.0 39.0 2.0 40.0 22 30.08105562139201 36.0 27.0 39.0 2.0 40.0 23 29.819917522628856 36.0 27.0 39.0 2.0 40.0 24 29.575222476036764 36.0 27.0 39.0 2.0 41.0 25 29.000130260871224 36.0 26.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4313604866837011 1101 2 0.24938802351627487 1101 3 0.5214677338835543 1101 4 0.7752995669944944 1101 5 0.3319731723610708 1101 6 0.32678949142461633 1101 7 0.012989392447934733 1101 8 -0.6051830382790975 1101 9 -0.12053433317133155 1101 10 0.22443239279929017 1101 11 0.17034570153047923 1101 12 -0.09742749355753233 1101 13 -0.14833872884335264 1101 14 -0.4519227053040069 1101 15 -0.6094746881425408 1101 16 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1103 3 0.18249821594537607 1103 4 0.017081825749528434 1103 5 -0.1780876205150328 1103 6 0.013534931958908203 1103 7 0.19239473537112772 1103 8 0.13547326791150383 1103 9 0.34739100290196134 1103 10 0.30821311032647714 1103 11 0.013846857451362382 1103 12 0.06674752142662754 1103 13 0.10654824778135819 1103 14 -0.027209544930688168 1103 15 -0.10658274533921741 1103 16 0.10838222072427683 1103 17 -0.6297810765964797 1103 18 0.15479437807696428 1103 19 -0.03666019532591136 1103 20 -0.17317007764801673 1103 21 0.41185160092952344 1103 22 0.46339291821490036 1103 23 0.5346976459416091 1103 24 0.501027307624458 1103 25 0.705585512064637 1104 1 0.22380851852801698 1104 2 0.36260120352512004 1104 3 0.526211210221458 1104 4 0.28495237548631636 1104 5 0.6972097933252464 1104 6 0.7304604334856499 1104 7 0.4744776719702699 1104 8 0.642837748983176 1104 9 1.1593860560369826 1104 10 1.1536783016891619 1104 11 0.9885456130740451 1104 12 1.0693128868051396 1104 13 0.9797793604976448 1104 14 1.4471816732895348 1104 15 1.4194616232241124 1104 16 1.6758948888574956 1104 17 1.7210610943330273 1104 18 1.799149808136832 1104 19 1.8253879381081148 1104 20 1.9140742340044739 1104 21 1.977359256531411 1104 22 1.8293602382617422 1104 23 1.9263208086518766 1104 24 2.0291248440419203 1104 25 1.6111179019914204 1105 1 -0.4915556494677311 1105 2 -0.4531351069480323 1105 3 -0.47179896673608823 1105 4 -0.45509471192919193 1105 5 -0.4570090872094248 1105 6 -0.1975717682307625 1105 7 0.063343776625846 1105 8 -0.14160821441015514 1105 9 -0.055590779992485295 1105 10 -0.07834114085659749 1105 11 -0.12107692812744375 1105 12 -0.09593356265183672 1105 13 -0.15609061747759512 1105 14 -0.2974982082324509 1105 15 -0.33214466522428054 1105 16 -0.2876807168233775 1105 17 -0.373311200039268 1105 18 -0.0791671964959626 1105 19 -0.08753072217896829 1105 20 -0.4957533652968067 1105 21 -0.277140629547425 1105 22 -0.4025528778619005 1105 23 0.16813747152117386 1105 24 -0.03479252513860942 1105 25 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-0.6975718554839361 2315 2 -0.7041618579747819 2315 3 -0.7606212555583767 2315 4 -0.7610665179009999 2315 5 -0.9173265475268835 2315 6 -1.3802779509369465 2315 7 -1.2239800106979395 2315 8 -0.935203508005447 2315 9 -0.9112906356923425 2315 10 -1.0311105936260496 2315 11 -0.8903631974137127 2315 12 -0.874932341650613 2315 13 -0.856445817832796 2315 14 -1.2730226837569276 2315 15 -0.9978123308919464 2315 16 -0.8676562967989554 2315 17 -1.459891286619353 2315 18 -1.032602649931416 2315 19 -0.8316199662682138 2315 20 -1.0194518889953308 2315 21 -0.8334176858244788 2315 22 -0.5844820597910854 2315 23 -0.8835885802048793 2315 24 -0.7414747318208157 2315 25 -0.7957261311684007 2316 1 -0.37781784355022197 2316 2 -0.788365230863679 2316 3 -0.39797779807298994 2316 4 -0.5606282983280053 2316 5 -0.6835891257694939 2316 6 -0.6743360835974066 2316 7 -0.4595245548947311 2316 8 -0.8380424882154749 2316 9 -0.8708817932868271 2316 10 -0.951947660053797 2316 11 -0.6352968549929621 2316 12 -1.0687302243004098 2316 13 -1.0191447384352088 2316 14 -1.4296877048146257 2316 15 -1.261591258564387 2316 16 -0.73838457018946 2316 17 -0.8785585871502128 2316 18 -0.46260941521272514 2316 19 -0.24914448279439227 2316 20 -0.4562208881603347 2316 21 -0.43935223934638756 2316 22 -0.46765601284856473 2316 23 -0.1524201368900151 2316 24 -0.2516653177185546 2316 25 -0.6050030993472006 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38876.0 3 4.0 4 592.0 5 4291.0 6 5313.0 7 4369.0 8 3419.0 9 3032.0 10 2915.0 11 2671.0 12 2563.0 13 2511.0 14 2447.0 15 2403.0 16 2507.0 17 2525.0 18 2717.0 19 3139.0 20 3820.0 21 4866.0 22 6120.0 23 7338.0 24 8657.0 25 9946.0 26 11243.0 27 12982.0 28 14820.0 29 17812.0 30 21650.0 31 26641.0 32 32838.0 33 40007.0 34 48715.0 35 60070.0 36 73393.0 37 83620.0 38 58674.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.31857600142833 24.777938607118717 15.85913687781363 20.04434851363932 2 16.672756097638466 24.899524388965315 40.64774601036368 17.779973503032537 3 21.601859235654626 28.055332276419925 31.016701985366304 19.32610650255915 4 16.360924280308687 20.875575451226837 37.49447979844513 25.26902047001935 5 12.356972496452617 41.03715497420301 35.800056249672274 10.805816279672099 6 30.59621353887016 40.60501409041375 17.14709629455478 11.651676076161307 7 27.173847429571758 34.03049375224382 22.876827226428343 15.918831591756074 8 23.900487048574597 42.38990573560856 19.808230580804633 13.901376635012216 9 26.469326741921446 16.10024368314202 22.06031396047059 35.370115614465945 10 15.841945906790404 31.84640019316734 34.079262151591884 18.23239174845037 11 33.25432958542095 22.88095744917443 27.080123144179723 16.784589821224895 12 24.474746865002082 26.44502196960791 32.59714760462966 16.483083560760342 13 30.631002722769917 23.734960429288996 26.12048177459786 19.513555073343227 14 19.862241185945805 26.393711894723797 28.136189329410684 25.607857589919714 15 21.80360473132901 37.215530908363064 23.03870018713086 17.942164173177062 16 19.671138956578652 30.753638567384584 31.157447268175364 18.417775207861403 17 20.01299431617808 32.76569246361433 29.245471846177796 17.975841374029795 18 19.792345108704286 28.397664200182366 34.70419662401947 17.10579406709388 19 23.54592331129489 27.44342389111462 30.34919444770979 18.6614583498807 20 23.526225325890458 29.150000158854724 30.65213040066338 16.67164411459144 21 21.205040142587997 28.193218174250923 29.51457174355765 21.087169939603434 22 22.54466200481012 30.891683319936586 29.962383202066384 16.601271473186912 23 19.994884877983687 31.12138724650758 31.14442118105308 17.739306694455653 24 22.90748618758201 28.417362185586793 31.73091281099783 16.94423881583337 25 20.955580974168335 30.316302862771266 31.44155307724081 17.28656308581959 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 305.0 1 305.0 2 365.5 3 426.0 4 426.0 5 426.0 6 1040.0 7 1654.0 8 1654.0 9 1654.0 10 2264.5 11 2875.0 12 2875.0 13 2875.0 14 3747.0 15 4619.0 16 4619.0 17 4619.0 18 8149.5 19 11680.0 20 11680.0 21 11680.0 22 18838.5 23 25997.0 24 25997.0 25 25997.0 26 37465.0 27 48933.0 28 48933.0 29 48933.0 30 60811.0 31 72689.0 32 72689.0 33 72689.0 34 77965.0 35 83241.0 36 83241.0 37 83241.0 38 82023.0 39 80805.0 40 80805.0 41 80805.0 42 79915.5 43 79026.0 44 79026.0 45 79026.0 46 75866.5 47 72707.0 48 72707.0 49 72707.0 50 65546.5 51 58386.0 52 58386.0 53 58386.0 54 47593.0 55 36800.0 56 36800.0 57 36800.0 58 29989.0 59 23178.0 60 23178.0 61 23178.0 62 18334.5 63 13491.0 64 13491.0 65 13491.0 66 10355.5 67 7220.0 68 7220.0 69 7220.0 70 5317.0 71 3414.0 72 3414.0 73 3414.0 74 2367.5 75 1321.0 76 1321.0 77 1321.0 78 916.5 79 512.0 80 512.0 81 512.0 82 341.0 83 170.0 84 170.0 85 170.0 86 105.0 87 40.0 88 40.0 89 40.0 90 26.5 91 13.0 92 13.0 93 13.0 94 7.0 95 1.0 96 1.0 97 1.0 98 2.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34979809564960457 2 0.0 3 0.0 4 0.0 5 0.026846447849583642 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.007307317166158862 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 629506.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.49210489607684 #Duplication Level Percentage of deduplicated Percentage of total 1 91.77110905321143 67.44451972967921 2 5.468778130208437 8.038240319972989 3 1.1936127972711261 2.631633507070479 4 0.5116714283203575 1.504152411297807 5 0.26636284009167693 0.978778289221723 6 0.146295592031263 0.6450942597237146 7 0.10516692258229618 0.5410256954210992 8 0.07485159479774589 0.44008010052116653 9 0.0670797819140825 0.44368509319521376 >10 0.32521740743558747 4.411121553874174 >50 0.03212447325407033 1.6445446787973 >100 0.029968383226389405 4.36083033596771 >500 0.004096397707127938 1.8926719846895712 >1k 0.0036651979484828923 5.02362204056784 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4307 0.6841872833618743 No Hit GGTATCAACGCAGAGTACTTTTTTT 3369 0.5351815550606348 No Hit TATCAACGCAGAGTACTTTTTTTTT 3314 0.5264445454054449 No Hit CTGTAGGACGTGGAATATGGCAAGA 2738 0.4349442261074557 No Hit GTCCTACAGTGGACATTTCTAAATT 2103 0.3340714782702627 No Hit CTTTAGGACGTGAAATATGGCGAGG 1996 0.3170740231228932 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1918 0.30468335488462384 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1699 0.2698941709848675 No Hit CTGAAGGACCTGGAATATGGCGAGA 1389 0.22064920747379693 No Hit CTGTAGGACCTGGAATATGGCGAGA 1314 0.2087351033985379 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1188 0.18871940855210276 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1130 0.17950583473390244 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1129 0.179346980012899 No Hit GTCCTACAGTGTGCATTTCTCATTT 1052 0.1671151664956331 No Hit GGAATATGGCGAGAAAACTGAAAAT 1030 0.1636203626335571 No Hit GAATATGGCAAGAAAACTGAAAATC 1018 0.16171410598151567 No Hit ATTTAGAAATGTCCACTGTAGGACG 1011 0.16060212293449147 No Hit ACAGTGGACATTTCTAAATTTTCCA 884 0.14042757336705292 No Hit ACCTGGAATATGGCGAGAAAACTGA 843 0.13391452980591131 No Hit GAAATATGGCGAGGAAAACTGAAAA 766 0.12168271628864538 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 761 0.12088844268362811 No Hit CTGTAGGACATGGAATATGGCAAGA 695 0.11040403109740018 No Hit GACCTGGAATATGGCGAGAAAACTG 685 0.10881548388736566 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.588547210034535E-4 0.0 0.0 0.0 3 0.0 1.588547210034535E-4 0.0 0.0 0.0 4 0.0 1.588547210034535E-4 0.0 0.0 0.0 5 0.0 1.588547210034535E-4 0.0 0.0 0.0 6 0.0 1.588547210034535E-4 0.0 0.0 0.0 7 0.0 1.588547210034535E-4 0.0 0.0 0.0 8 0.0 1.588547210034535E-4 0.0 0.0 0.0 9 0.0 1.588547210034535E-4 0.0 0.0 0.0 10 0.0 1.588547210034535E-4 0.0 0.0 0.0 11 0.0 1.588547210034535E-4 0.0 0.0 0.0 12 0.0 1.588547210034535E-4 0.0 0.0 0.0 13 0.0 1.588547210034535E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACGAG 45 6.7675003E-4 14.774009 6 TACACGG 65 3.3650103E-6 14.61514 5 GGTATCA 725 0.0 14.595606 1 TAGACAG 95 4.8803486E-9 13.999766 5 AGGACGA 50 0.0014980078 13.299778 5 GACTGTG 100 1.4418947E-7 12.34685 7 TTAGGGC 55 0.0030653428 12.090708 3 TAGGGCG 55 0.0030653428 12.090708 4 TAGGACC 480 0.0 12.072716 4 AGACTGT 80 2.8674938E-5 11.871971 6 TAGAAAT 430 0.0 11.709106 4 CTCTAGA 65 8.028954E-4 11.689326 9 AAATGTC 385 0.0 11.594445 7 ATAATTC 75 2.0704E-4 11.39981 3 CCACCTT 370 0.0 11.294416 13 CACCTTT 375 0.0 11.143824 14 AGGACCT 905 0.0 11.126886 5 GTGGTAT 180 3.6379788E-12 11.122026 1 TATACAC 205 0.0 11.121765 3 CAGTCCT 60 0.0058802306 11.080507 9 >>END_MODULE