FastQCFastQC Report
Thu 26 May 2016
SRR1512158_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512158_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822606
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT40130.48783986501435683No Hit
CTGTAGGACGTGGAATATGGCAAGA36410.44261772950841594No Hit
GTATCAACGCAGAGTACTTTTTTTT33490.4071207844338602No Hit
GTCCTAAAGTGTGTATTTCTCATTT24550.2984417813631313No Hit
GGTATCAACGCAGAGTACTTTTTTT21730.26416048509249873No Hit
TATCAACGCAGAGTACTTTTTTTTT19750.2400906387748205No Hit
CTTTAGGACGTGAAATATGGCGAGG19080.23194579178853544No Hit
GTCCTACAGTGTGCATTTCTCATTT18470.2245303340846043No Hit
CTGTAGGACCTGGAATATGGCGAGA12540.15244236001196199No Hit
CTGAAGGACCTGGAATATGGCGAGA10740.13056068154134542No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10450.12703530000996832No Hit
GTGTATATCAATGAGTTACAATGAA9810.11925514766486021No Hit
TTGTAGAACAGTGTATATCAATGAG9500.11548663637269847No Hit
GATATACACTGTTCTACAAATCCCG9330.11342003340602913No Hit
GTCCTTCAGTGTGCATTTCTCATTT9300.11305533876485219No Hit
ATTCCAGGTCCTTCAGTGTGCATTT8590.10442423225699789No Hit
GAATATGGCAAGAAAACTGAAAATC8370.10174980488836696No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC308.066136E-418.8759278
CCGTAGG400.003623388215.0791081
CCAACGT704.7597132E-714.8337919
TAGGACC4650.014.817494
TCCAACG2250.014.68395418
ACACCGT400.005508421514.1578066
GACGTGT400.005508421514.1578067
CCAACGA1600.013.56954819
TGGACCC500.001573598113.2139535
TGTAGAA4850.012.8509242
GGTATCA5800.012.8259091
CCAGTAC905.8362093E-712.5847173
TGTAGGA16650.012.5583762
ACTGTTC4750.012.5177198
GTCCTAC16650.012.2564931
CCTACCC854.233152E-612.2145783
GACGTGG9200.012.2085447
CCGGTTT1405.820766E-1112.13673712
AGGACGT16200.012.1186165
CTGTAGG16100.012.1132381
Produced by FastQC (version 0.11.5)