##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512158_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 822606 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.955783206054903 31.0 30.0 34.0 25.0 34.0 2 27.432807443660757 31.0 28.0 34.0 10.0 34.0 3 28.92024736021862 31.0 28.0 34.0 16.0 34.0 4 33.33178338110833 35.0 33.0 37.0 28.0 37.0 5 33.701519317875146 37.0 35.0 37.0 30.0 37.0 6 33.91658704167001 37.0 35.0 37.0 30.0 37.0 7 33.93038465559454 37.0 35.0 37.0 30.0 37.0 8 33.93729440339604 37.0 35.0 37.0 30.0 37.0 9 35.5250666783369 39.0 37.0 39.0 30.0 39.0 10 35.40672691422139 39.0 37.0 39.0 30.0 39.0 11 35.417864688562936 39.0 37.0 39.0 30.0 39.0 12 35.324782459646535 39.0 37.0 39.0 30.0 39.0 13 35.3388997892065 39.0 37.0 39.0 30.0 39.0 14 36.48619509218265 40.0 37.0 41.0 30.0 41.0 15 36.441279300175296 40.0 37.0 41.0 30.0 41.0 16 36.39825870465326 40.0 37.0 41.0 30.0 41.0 17 36.40796444470378 40.0 37.0 41.0 30.0 41.0 18 36.3805150947112 40.0 37.0 41.0 30.0 41.0 19 36.41289997884771 40.0 37.0 41.0 30.0 41.0 20 36.360373009678995 40.0 37.0 41.0 30.0 41.0 21 36.28850385239106 40.0 37.0 41.0 29.0 41.0 22 36.2024055258532 40.0 37.0 41.0 29.0 41.0 23 36.106885921084945 40.0 37.0 41.0 29.0 41.0 24 36.03819082282405 40.0 37.0 41.0 29.0 41.0 25 35.929125243433674 40.0 37.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.242315216581801 1101 2 3.2922898532350047 1101 3 1.802867441698762 1101 4 0.6180684927181943 1101 5 0.2833651819707086 1101 6 0.31361249923467227 1101 7 0.030561804826966465 1101 8 -0.057848451370773546 1101 9 -0.08524393734914781 1101 10 -0.0258265776050024 1101 11 0.10916469876654133 1101 12 0.01698526043580273 1101 13 -0.05939618455872164 1101 14 -0.025787315743222905 1101 15 0.014650449091746509 1101 16 0.1976332180357403 1101 17 0.09254737040964756 1101 18 0.3242880755842208 1101 19 0.11421380066293096 1101 20 0.1794385118527302 1101 21 0.06869291210591655 1101 22 0.12608335154256167 1101 23 0.20071369275904516 1101 24 0.3030541080465099 1101 25 0.2874754760750662 1102 1 1.4136783720563209 1102 2 4.490934769096949 1102 3 2.669159804156436 1102 4 1.8166201540950837 1102 5 1.260009756971428 1102 6 1.2591004292102 1102 7 1.1886699607356022 1102 8 1.1210136509712 1102 9 1.1911237683011109 1102 10 1.2969175481663555 1102 11 1.0956524454487706 1102 12 1.3755680495166658 1102 13 1.2913564434491391 1102 14 1.516937708937057 1102 15 1.2988836882058905 1102 16 1.4076326713715162 1102 17 1.4207388443771762 1102 18 1.4172531501717316 1102 19 1.3127558150241185 1102 20 1.321455445580952 1102 21 1.3843786380599212 1102 22 1.3541388025887784 1102 23 1.3865377247614177 1102 24 1.3741429667080851 1102 25 1.3019041958839281 1103 1 1.406594416109673 1103 2 4.318625898678452 1103 3 2.868825258915461 1103 4 1.8766124060182676 1103 5 1.3238037620381746 1103 6 1.2233135758668396 1103 7 1.1604595067050738 1103 8 1.0948509029565017 1103 9 1.134995389670891 1103 10 1.3027263468643184 1103 11 1.2551543999745647 1103 12 1.2504479035004294 1103 13 1.4639939859308484 1103 14 1.4570832507998261 1103 15 1.626010653885075 1103 16 1.5257919722826543 1103 17 1.3591246918258975 1103 18 1.3529486304226026 1103 19 1.4507935637447247 1103 20 1.6303123105916058 1103 21 1.610076953248523 1103 22 1.5551573471475635 1103 23 1.7576751221078695 1103 24 1.6897449504868192 1103 25 1.6124337870994552 1104 1 1.4556001381650816 1104 2 4.244905152094166 1104 3 2.715960011065711 1104 4 1.7992230229804775 1104 5 1.4000866574976456 1104 6 1.042578021773238 1104 7 1.0506825269652467 1104 8 1.0161769768680244 1104 9 1.0555144329349062 1104 10 1.0102101108645059 1104 11 0.9743886689470074 1104 12 1.0792972836014627 1104 13 1.2028721670826386 1104 14 1.3997181711778168 1104 15 1.07585971140508 1104 16 1.3919243183716148 1104 17 1.2701478286314654 1104 18 1.2854401698971571 1104 19 1.4890361793564892 1104 20 1.4524568615738644 1104 21 1.522417006827034 1104 22 1.4187922918058362 1104 23 1.6268841280149147 1104 24 1.509135422669118 1104 25 1.5735009174811552 1105 1 0.8872203051566139 1105 2 3.898669443834777 1105 3 2.395273425664378 1105 4 1.5526294757195487 1105 5 1.0585069292225455 1105 6 0.7803838577491504 1105 7 0.549704433694366 1105 8 0.6145652467309972 1105 9 0.6338997935377009 1105 10 0.8300314822897548 1105 11 0.6816472730839749 1105 12 0.5661270627886168 1105 13 0.9367004625471012 1105 14 0.7577573298197677 1105 15 0.7554477601642802 1105 16 0.5559658626069179 1105 17 0.8845533213146766 1105 18 0.7419798958448496 1105 19 0.631963476944712 1105 20 0.6966008117673255 1105 21 0.7481220660304828 1105 22 0.8545633912152582 1105 23 0.876037824229968 1105 24 0.7758435947809517 1105 25 0.6216165896657202 1106 1 -0.05321152331990575 1106 2 2.8496221842847262 1106 3 1.4395373543975793 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-1.098395215080643 2315 20 -0.9209550774552326 2315 21 -0.8894705265621141 2315 22 -0.8964843231657866 2315 23 -0.7383649118394331 2315 24 -0.7057096251056052 2315 25 -0.5871988924830944 2316 1 -1.883629005692697 2316 2 -0.6218479966826642 2316 3 -0.7962072858163332 2316 4 -1.3669023638122937 2316 5 -1.810455879740264 2316 6 -1.6526568752915836 2316 7 -2.0105265665366368 2316 8 -1.6274921953264467 2316 9 -1.8990916594537524 2316 10 -1.9459891537308849 2316 11 -2.5385652471285454 2316 12 -1.9708868742253216 2316 13 -1.9828128969662586 2316 14 -2.2657831337206957 2316 15 -2.7248879601714364 2316 16 -2.453651447010394 2316 17 -2.2498687931074386 2316 18 -2.6953543614894073 2316 19 -2.8157503907691535 2316 20 -2.921073785907268 2316 21 -2.6177468798384638 2316 22 -2.610396953745088 2316 23 -2.4087115405193984 2316 24 -2.2162746773373243 2316 25 -2.21777818972906 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28946.0 3 0.0 4 265.0 5 1190.0 6 1328.0 7 1142.0 8 1135.0 9 1037.0 10 1003.0 11 933.0 12 900.0 13 878.0 14 877.0 15 927.0 16 991.0 17 1007.0 18 1157.0 19 1446.0 20 1903.0 21 2385.0 22 2797.0 23 3282.0 24 3913.0 25 4434.0 26 4978.0 27 5832.0 28 6873.0 29 8530.0 30 10511.0 31 14364.0 32 19370.0 33 27441.0 34 40352.0 35 61322.0 36 96828.0 37 133910.0 38 328419.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.599988329771485 19.894578935723786 13.28862177032504 25.216810964179693 2 14.61504698054357 20.33104548379537 43.771727773095044 21.282179762566017 3 20.0114027857808 23.41120779571265 29.477781586810696 27.099607831695856 4 11.33081937160682 16.634451973362705 39.401244338115696 32.63348431691478 5 11.712533096038687 38.592108494224455 36.947092532755654 12.748265876981204 6 30.143264385701524 38.974598987363315 18.146619580479086 12.735517046456076 7 27.02140825960782 31.668638218770557 22.15522052002777 19.154733001593858 8 22.57593550253706 41.19675762151018 20.586404670036444 15.64090220591632 9 24.950948570761703 16.157066688062084 21.49121207479644 37.40077266637977 10 14.13642480879884 30.299077395895512 35.29504730944621 20.26945048585944 11 33.11698179267452 22.69174707414832 25.96400840768265 18.227262725494512 12 23.017028849847556 24.620595085849327 33.53265970017165 18.829716364131464 13 28.889286000831504 22.787944653941256 26.37678305288315 21.945986292344084 14 18.44534970088852 24.42794397783621 28.38550309201401 28.74120322926126 15 21.03108900251153 34.47871763638972 23.86561731861912 20.62457604247963 16 19.154005684373807 29.45176670240674 31.753354582874426 19.640873030345023 17 19.020915263097113 30.479513253627193 30.56716163893971 19.932409844335982 18 18.630347507434777 28.384434507091918 34.239002244415104 18.746215741058204 19 21.702833801378606 27.269654862200177 29.338536537923826 21.688974798497394 20 21.357612271245287 28.841511002837322 31.036608048081344 18.76426867783605 21 19.93116452553733 28.1895978437549 28.893757271673998 22.985480359033776 22 20.07359654166763 30.936805929542317 29.787999654750287 19.201597874039773 23 19.43908191300542 29.797952782708066 30.50378078727905 20.259184517007466 24 21.222875511851612 27.52120354429887 32.233034732961784 19.02288621088773 25 20.15109369073208 28.880438059853912 31.311699427137533 19.656768822276472 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 131.0 1 131.0 2 214.0 3 297.0 4 297.0 5 297.0 6 774.0 7 1251.0 8 1251.0 9 1251.0 10 2000.5 11 2750.0 12 2750.0 13 2750.0 14 4081.5 15 5413.0 16 5413.0 17 5413.0 18 8872.0 19 12331.0 20 12331.0 21 12331.0 22 19570.0 23 26809.0 24 26809.0 25 26809.0 26 38819.0 27 50829.0 28 50829.0 29 50829.0 30 67306.5 31 83784.0 32 83784.0 33 83784.0 34 91825.5 35 99867.0 36 99867.0 37 99867.0 38 102001.0 39 104135.0 40 104135.0 41 104135.0 42 106253.5 43 108372.0 44 108372.0 45 108372.0 46 107099.5 47 105827.0 48 105827.0 49 105827.0 50 96272.0 51 86717.0 52 86717.0 53 86717.0 54 72458.0 55 58199.0 56 58199.0 57 58199.0 58 47689.0 59 37179.0 60 37179.0 61 37179.0 62 29193.0 63 21207.0 64 21207.0 65 21207.0 66 15918.0 67 10629.0 68 10629.0 69 10629.0 70 7513.0 71 4397.0 72 4397.0 73 4397.0 74 3029.0 75 1661.0 76 1661.0 77 1661.0 78 1113.5 79 566.0 80 566.0 81 566.0 82 375.0 83 184.0 84 184.0 85 184.0 86 116.0 87 48.0 88 48.0 89 48.0 90 30.0 91 12.0 92 12.0 93 12.0 94 9.5 95 7.0 96 7.0 97 7.0 98 5.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 6.110458712919672 3 0.0 4 0.0 5 0.0 6 1.2156488039231418E-4 7 0.008874236268638936 8 0.0 9 0.0 10 0.00522728985686951 11 0.003039122009807854 12 2.4312976078462835E-4 13 0.0 14 3.6469464117694253E-4 15 0.0 16 0.0 17 1.2156488039231418E-4 18 0.014587785647077701 19 0.00437633569412331 20 0.0 21 0.007901717225500421 22 0.0015803434451000845 23 0.003160686890200169 24 0.012399617800016048 25 0.008509541627461993 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 822606.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.2164236116717 #Duplication Level Percentage of deduplicated Percentage of total 1 92.39122617403892 72.26511284430399 2 5.539623055827917 8.665790071672392 3 0.9161232330360505 2.1496764862692586 4 0.3574532478238687 1.1183485861263833 5 0.1918166099419361 0.7501604609486636 6 0.1149319184332062 0.5393738171204253 7 0.08406522132498183 0.46026966735146013 8 0.06051559190705534 0.3786650537370634 9 0.04245334401104906 0.29884938650102155 >10 0.25048344352928137 3.638352632784523 >50 0.025102770321879946 1.3331460969327016 >100 0.02083802086393708 3.333342260026821 >500 0.003630867224094065 2.014871782079414 >1k 0.001736501715871075 3.0540408541458652 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 4013 0.48783986501435683 No Hit CTGTAGGACGTGGAATATGGCAAGA 3641 0.44261772950841594 No Hit GTATCAACGCAGAGTACTTTTTTTT 3349 0.4071207844338602 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2455 0.2984417813631313 No Hit GGTATCAACGCAGAGTACTTTTTTT 2173 0.26416048509249873 No Hit TATCAACGCAGAGTACTTTTTTTTT 1975 0.2400906387748205 No Hit CTTTAGGACGTGAAATATGGCGAGG 1908 0.23194579178853544 No Hit GTCCTACAGTGTGCATTTCTCATTT 1847 0.2245303340846043 No Hit CTGTAGGACCTGGAATATGGCGAGA 1254 0.15244236001196199 No Hit CTGAAGGACCTGGAATATGGCGAGA 1074 0.13056068154134542 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1045 0.12703530000996832 No Hit GTGTATATCAATGAGTTACAATGAA 981 0.11925514766486021 No Hit TTGTAGAACAGTGTATATCAATGAG 950 0.11548663637269847 No Hit GATATACACTGTTCTACAAATCCCG 933 0.11342003340602913 No Hit GTCCTTCAGTGTGCATTTCTCATTT 930 0.11305533876485219 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 859 0.10442423225699789 No Hit GAATATGGCAAGAAAACTGAAAATC 837 0.10174980488836696 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2156488039231418E-4 2 0.0 0.0 0.0 0.0 1.2156488039231418E-4 3 0.0 0.0 0.0 0.0 1.2156488039231418E-4 4 0.0 0.0 0.0 0.0 1.2156488039231418E-4 5 0.0 0.0 0.0 0.0 1.2156488039231418E-4 6 0.0 0.0 0.0 0.0 1.2156488039231418E-4 7 0.0 0.0 0.0 0.0 1.2156488039231418E-4 8 0.0 0.0 0.0 1.2156488039231418E-4 1.2156488039231418E-4 9 0.0 0.0 0.0 1.2156488039231418E-4 1.2156488039231418E-4 10 0.0 0.0 0.0 1.2156488039231418E-4 1.2156488039231418E-4 11 0.0 0.0 0.0 1.2156488039231418E-4 1.2156488039231418E-4 12 0.0 0.0 0.0 2.4312976078462835E-4 2.4312976078462835E-4 13 0.0 0.0 0.0 3.6469464117694253E-4 3.6469464117694253E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTGC 30 8.066136E-4 18.875927 8 CCGTAGG 40 0.0036233882 15.079108 1 CCAACGT 70 4.7597132E-7 14.83379 19 TAGGACC 465 0.0 14.81749 4 TCCAACG 225 0.0 14.683954 18 ACACCGT 40 0.0055084215 14.157806 6 GACGTGT 40 0.0055084215 14.157806 7 CCAACGA 160 0.0 13.569548 19 TGGACCC 50 0.0015735981 13.213953 5 TGTAGAA 485 0.0 12.850924 2 GGTATCA 580 0.0 12.825909 1 CCAGTAC 90 5.8362093E-7 12.584717 3 TGTAGGA 1665 0.0 12.558376 2 ACTGTTC 475 0.0 12.517719 8 GTCCTAC 1665 0.0 12.256493 1 CCTACCC 85 4.233152E-6 12.214578 3 GACGTGG 920 0.0 12.208544 7 CCGGTTT 140 5.820766E-11 12.136737 12 AGGACGT 1620 0.0 12.118616 5 CTGTAGG 1610 0.0 12.113238 1 >>END_MODULE