FastQCFastQC Report
Thu 26 May 2016
SRR1512158_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512158_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822606
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51700.6284904316282643No Hit
GGTATCAACGCAGAGTACTTTTTTT39070.47495398769277153No Hit
TATCAACGCAGAGTACTTTTTTTTT36460.4432255539103775No Hit
CTGTAGGACGTGGAATATGGCAAGA33580.408214868357391No Hit
GTCCTACAGTGGACATTTCTAAATT27790.3378288026102411No Hit
CTTTAGGACGTGAAATATGGCGAGG19560.23778090604736654No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19210.23352613523363552No Hit
GTCCTAAAGTGTGTATTTCTCATTT18300.22246373111793494No Hit
CTGAAGGACCTGGAATATGGCGAGA13730.16690858077864737No Hit
CTGTAGGACCTGGAATATGGCGAGA13600.1653282373335473No Hit
GTCCTACAGTGTGCATTTCTCATTT13260.1611950314002086No Hit
TTGTAGAACAGTGTATATCAATGAG12610.15329331417470818No Hit
GATATACACTGTTCTACAAATCCCG10410.12654904048839907No Hit
GAATATGGCAAGAAAACTGAAAATC9830.11949827742564485No Hit
ACCTGGAATATGGCGAGAAAACTGA9590.1165807202962293No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9270.11269064412367524No Hit
ATTCCAGGTCCTTCAGTGTGCATTT8820.1072202245060211No Hit
GGAATATGGCGAGAAAACTGAAAAT8660.10527518641974408No Hit
ACAGTGGACATTTCTAAATTTTCCA8560.10405953761582094No Hit
GTGGTATCAACGCAGAGTACTTTTT8490.10320858345307474No Hit
GACCTGGAATATGGCGAGAAAACTG8310.10102041560601308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA901.3460522E-1015.83049719
TCCAACG1350.014.77423118
GGTATCA10800.014.3004731
CGGTTTC903.6419806E-813.7189313
TGCCGGT957.3932824E-812.996881510
TAGGACC4000.012.5867764
AATCCCG2050.012.509953519
GCCGGTT1001.4437137E-712.34703611
GTAGCAC802.8642049E-511.8743163
CTTATTC802.8642049E-511.8743163
TGTAGGA16600.011.7884772
CCGGTTT1052.7221358E-711.75908412
TCTATAC907.4532873E-611.6104423
TTGTAGA3550.011.54781
TGTAGAA5650.011.432972
GCCTCGA752.0761757E-411.397264516
CTGTAGG16350.011.3704051
ACTGTTC4850.011.3580998
GAACAGT2950.011.2684816
GTAGGAC16450.011.2607493
Produced by FastQC (version 0.11.5)