##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512158_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 822606 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.28440103767782 31.0 28.0 31.0 22.0 34.0 2 28.489050651223064 31.0 28.0 31.0 22.0 34.0 3 28.48383065525902 31.0 28.0 33.0 22.0 34.0 4 31.95762491399285 35.0 32.0 37.0 24.0 37.0 5 31.360791192867545 35.0 32.0 37.0 18.0 37.0 6 31.34951969715757 35.0 32.0 37.0 17.0 37.0 7 31.05467866755166 35.0 32.0 37.0 17.0 37.0 8 30.890200168732054 35.0 31.0 37.0 13.0 37.0 9 31.955839125899885 37.0 32.0 39.0 11.0 39.0 10 31.598950165692933 37.0 30.0 39.0 10.0 39.0 11 31.6463495282067 37.0 31.0 39.0 10.0 39.0 12 31.322972358577495 35.0 30.0 39.0 10.0 39.0 13 31.33371626270657 37.0 30.0 39.0 10.0 39.0 14 31.789307396250454 37.0 30.0 40.0 8.0 41.0 15 31.719510920173207 37.0 30.0 40.0 2.0 41.0 16 31.517253703474083 37.0 30.0 40.0 2.0 41.0 17 31.435270348137504 37.0 30.0 40.0 2.0 41.0 18 31.298316569536325 36.0 30.0 40.0 2.0 41.0 19 31.35432515687948 37.0 30.0 40.0 2.0 41.0 20 31.161841756563895 37.0 30.0 40.0 2.0 41.0 21 30.977679472311166 36.0 30.0 40.0 2.0 41.0 22 31.046108343484 37.0 30.0 40.0 2.0 41.0 23 30.836585193883828 37.0 30.0 40.0 2.0 41.0 24 30.583946628154912 37.0 30.0 40.0 2.0 41.0 25 30.012133390711956 36.0 28.0 39.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05351734697938326 1101 2 -0.1322318462640375 1101 3 0.06294398647102284 1101 4 0.011358286194688816 1101 5 -0.06213271437764334 1101 6 -0.18380832605161856 1101 7 -0.5520186123064335 1101 8 -0.8490262883709079 1101 9 -0.3826548072619005 1101 10 -0.3815879430682436 1101 11 -0.2960709865947315 1101 12 -0.3899047239211164 1101 13 -0.5012874053968837 1101 14 -0.7808980948354076 1101 15 -0.6538936687606878 1101 16 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0.4065158913314697 1103 4 0.8566837010862223 1103 5 0.28475350510290554 1103 6 0.5630916893867131 1103 7 0.7146134549740424 1103 8 0.8249828936054406 1103 9 0.8605942064913563 1103 10 0.8282759477641264 1103 11 0.5856637127574054 1103 12 0.9300147777601318 1103 13 0.6264467399271112 1103 14 1.0183707194020215 1103 15 0.49192522535095407 1103 16 0.9792188330811555 1103 17 0.7926269016089158 1103 18 1.0707564294040175 1103 19 0.923170405328932 1103 20 0.7098113410813127 1103 21 0.6891338240850651 1103 22 1.022423681397111 1103 23 1.0696701419699473 1103 24 0.48364500994745185 1103 25 0.8306753569647931 1104 1 0.46588740356074965 1104 2 0.4987679134328502 1104 3 0.5136867864302062 1104 4 0.6321095022491328 1104 5 0.7637759111873166 1104 6 0.7747476168418288 1104 7 1.1756927255015839 1104 8 0.7735815161035582 1104 9 1.0451637342628075 1104 10 1.0485074430022934 1104 11 1.1586230987666433 1104 12 1.326477254881734 1104 13 1.4593555535573692 1104 14 1.5160276712542071 1104 15 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2315 2 -0.40611620462852116 2315 3 -0.32961179561075227 2315 4 -0.5508231613078571 2315 5 -0.5375121457968284 2315 6 -0.6770125394393212 2315 7 -0.40423856926645385 2315 8 -0.4132747876346947 2315 9 -0.5108403322364161 2315 10 -0.5371548249348201 2315 11 -0.20983528606239332 2315 12 -0.2005712773246735 2315 13 -0.5174132407123189 2315 14 -0.4256908233437322 2315 15 -0.345152588230615 2315 16 -0.6146600440057455 2315 17 -0.5425745278907605 2315 18 -0.6541411319597081 2315 19 -0.5155752683791519 2315 20 -0.6241916558289553 2315 21 -0.3098119530562009 2315 22 -0.23035111273106423 2315 23 -0.34674125689759094 2315 24 -0.29463667309613584 2315 25 0.3218338086495578 2316 1 -1.1954185989723776 2316 2 -0.8889034790824617 2316 3 -1.0894645971302204 2316 4 -1.2616493721340696 2316 5 -1.6599005598519092 2316 6 -1.406357268784049 2316 7 -1.5450267933880113 2316 8 -1.0797040373972777 2316 9 -1.5044300758261606 2316 10 -1.9749515960649227 2316 11 -1.8032588378192784 2316 12 -1.9618485802686436 2316 13 -1.5830590146914254 2316 14 -1.9509757236286305 2316 15 -1.7757081437861721 2316 16 -1.6023143649934006 2316 17 -1.5875650312145986 2316 18 -1.4981582259768018 2316 19 -1.5917860945899776 2316 20 -1.5890539540245854 2316 21 -1.06622220946646 2316 22 -1.2545676369475913 2316 23 -1.3600366035262716 2316 24 -1.5747886199147487 2316 25 -1.5179097810940299 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42777.0 3 2.0 4 700.0 5 5166.0 6 6111.0 7 4986.0 8 4086.0 9 3687.0 10 3430.0 11 3248.0 12 2924.0 13 2790.0 14 2808.0 15 2862.0 16 2921.0 17 3009.0 18 3214.0 19 3587.0 20 4382.0 21 5551.0 22 7005.0 23 8761.0 24 10348.0 25 11788.0 26 13476.0 27 15496.0 28 17711.0 29 21072.0 30 25958.0 31 32405.0 32 40354.0 33 49825.0 34 62135.0 35 78587.0 36 100342.0 37 123617.0 38 95485.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.61403919617113 23.790304875371397 15.848494162531896 20.74716176592558 2 16.11585619360909 23.770310452391545 41.22386658983766 18.8899667641617 3 21.957291826220573 26.257892599859474 30.699994894275022 21.084820679644935 4 15.139690204058809 20.111936941865245 37.83670432746661 26.91166852660934 5 12.314509029537508 39.887063904709564 36.2728170308799 11.525610034873033 6 30.795909584904557 40.297299071487444 17.115241075314305 11.79155026829369 7 27.539551133835637 32.91065224420925 21.927751560285238 17.622045061669862 8 23.793894039187656 42.12697208627216 19.601972268619487 14.477161605920696 9 25.84736799877463 16.312061910562285 21.288563419182452 36.55200667148063 10 14.97120127983506 32.241316012769175 34.236438829767835 18.55104387762793 11 34.3144834829797 23.168321164688805 25.330352562466114 17.186842789865377 12 23.611546718599183 26.23090519641238 32.757480494914944 17.400067590073498 13 30.2622397599823 23.242354176847726 26.053298906159206 20.44210715701077 14 19.53438219512136 25.656632701439086 28.323158352844498 26.48582675059506 15 22.210633036958153 35.23861970372207 23.57361847591678 18.977128783402993 16 19.92618580462579 30.97935098941656 30.533694137898337 18.56076906805931 17 20.064648203392633 31.829940457521584 29.259694191386888 18.8457171476989 18 19.53195089751351 29.257627588420217 33.577557178041495 17.63286433602478 19 22.79402289796087 27.979008176453856 29.782423177073834 19.444545748511437 20 22.477346384538894 29.452617656569487 30.74132695360841 17.328709005283212 21 21.267532694874582 28.38783086921321 29.357310790341913 20.987325645570298 22 21.38253307172571 30.65951378910438 30.156843008682166 17.801110130487743 23 20.53473959586971 30.185775450215534 30.44592429425509 18.833560659659668 24 22.087001553599173 28.38843869361517 31.721383019331245 17.80317673345441 25 21.165189947285494 29.716100293722892 31.12879067867494 17.989919080316678 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 147.0 1 147.0 2 277.0 3 407.0 4 407.0 5 407.0 6 970.0 7 1533.0 8 1533.0 9 1533.0 10 2426.0 11 3319.0 12 3319.0 13 3319.0 14 4743.5 15 6168.0 16 6168.0 17 6168.0 18 10358.0 19 14548.0 20 14548.0 21 14548.0 22 22993.0 23 31438.0 24 31438.0 25 31438.0 26 44473.0 27 57508.0 28 57508.0 29 57508.0 30 73625.5 31 89743.0 32 89743.0 33 89743.0 34 96353.5 35 102964.0 36 102964.0 37 102964.0 38 104108.0 39 105252.0 40 105252.0 41 105252.0 42 105943.5 43 106635.0 44 106635.0 45 106635.0 46 103158.5 47 99682.0 48 99682.0 49 99682.0 50 90654.0 51 81626.0 52 81626.0 53 81626.0 54 67451.5 55 53277.0 56 53277.0 57 53277.0 58 43322.5 59 33368.0 60 33368.0 61 33368.0 62 26241.5 63 19115.0 64 19115.0 65 19115.0 66 14290.5 67 9466.0 68 9466.0 69 9466.0 70 6791.5 71 4117.0 72 4117.0 73 4117.0 74 2829.0 75 1541.0 76 1541.0 77 1541.0 78 1035.5 79 530.0 80 530.0 81 530.0 82 345.0 83 160.0 84 160.0 85 160.0 86 102.5 87 45.0 88 45.0 89 45.0 90 29.5 91 14.0 92 14.0 93 14.0 94 8.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33187212347101774 2 0.0 3 0.0 4 0.0 5 0.023583586796108953 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.007537022584323479 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 822606.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.93854725432097 #Duplication Level Percentage of deduplicated Percentage of total 1 93.10594527199282 72.56542115239435 2 4.905377934693982 7.646360599269006 3 0.8804881335073327 2.0587189800069035 4 0.3385659353448092 1.0554934860229914 5 0.17675831772110567 0.6888143249150337 6 0.10894133741065617 0.509443774423761 7 0.07779702036993033 0.42443707238430295 8 0.05878807119896635 0.36654854921048186 9 0.04448924247894802 0.31206842345289904 >10 0.25062299758736706 3.639320710754032 >50 0.024870575621122867 1.375494164073342 >100 0.021448933980282192 3.422742601778702 >500 0.0038856763767061995 2.188592816133531 >1k 0.0018651246608189756 3.120262011289505 >5k 1.5542705506824795E-4 0.6262813338910918 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5170 0.6284904316282643 No Hit GGTATCAACGCAGAGTACTTTTTTT 3907 0.47495398769277153 No Hit TATCAACGCAGAGTACTTTTTTTTT 3646 0.4432255539103775 No Hit CTGTAGGACGTGGAATATGGCAAGA 3358 0.408214868357391 No Hit GTCCTACAGTGGACATTTCTAAATT 2779 0.3378288026102411 No Hit CTTTAGGACGTGAAATATGGCGAGG 1956 0.23778090604736654 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1921 0.23352613523363552 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1830 0.22246373111793494 No Hit CTGAAGGACCTGGAATATGGCGAGA 1373 0.16690858077864737 No Hit CTGTAGGACCTGGAATATGGCGAGA 1360 0.1653282373335473 No Hit GTCCTACAGTGTGCATTTCTCATTT 1326 0.1611950314002086 No Hit TTGTAGAACAGTGTATATCAATGAG 1261 0.15329331417470818 No Hit GATATACACTGTTCTACAAATCCCG 1041 0.12654904048839907 No Hit GAATATGGCAAGAAAACTGAAAATC 983 0.11949827742564485 No Hit ACCTGGAATATGGCGAGAAAACTGA 959 0.1165807202962293 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 927 0.11269064412367524 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 882 0.1072202245060211 No Hit GGAATATGGCGAGAAAACTGAAAAT 866 0.10527518641974408 No Hit ACAGTGGACATTTCTAAATTTTCCA 856 0.10405953761582094 No Hit GTGGTATCAACGCAGAGTACTTTTT 849 0.10320858345307474 No Hit GACCTGGAATATGGCGAGAAAACTG 831 0.10102041560601308 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.2156488039231418E-4 0.0 8 0.0 0.0 0.0 1.2156488039231418E-4 0.0 9 0.0 0.0 0.0 1.2156488039231418E-4 0.0 10 0.0 0.0 0.0 1.2156488039231418E-4 0.0 11 0.0 0.0 0.0 1.2156488039231418E-4 0.0 12 0.0 0.0 0.0 1.2156488039231418E-4 0.0 13 0.0 0.0 0.0 1.2156488039231418E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 90 1.3460522E-10 15.830497 19 TCCAACG 135 0.0 14.774231 18 GGTATCA 1080 0.0 14.300473 1 CGGTTTC 90 3.6419806E-8 13.71893 13 TGCCGGT 95 7.3932824E-8 12.9968815 10 TAGGACC 400 0.0 12.586776 4 AATCCCG 205 0.0 12.5099535 19 GCCGGTT 100 1.4437137E-7 12.347036 11 GTAGCAC 80 2.8642049E-5 11.874316 3 CTTATTC 80 2.8642049E-5 11.874316 3 TGTAGGA 1660 0.0 11.788477 2 CCGGTTT 105 2.7221358E-7 11.759084 12 TCTATAC 90 7.4532873E-6 11.610442 3 TTGTAGA 355 0.0 11.5478 1 TGTAGAA 565 0.0 11.43297 2 GCCTCGA 75 2.0761757E-4 11.3972645 16 CTGTAGG 1635 0.0 11.370405 1 ACTGTTC 485 0.0 11.358099 8 GAACAGT 295 0.0 11.268481 6 GTAGGAC 1645 0.0 11.260749 3 >>END_MODULE