FastQCFastQC Report
Thu 26 May 2016
SRR1512157_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512157_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358954
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT57130.4203968640586804No Hit
CTGTAGGACGTGGAATATGGCAAGA48260.3551260749076128No Hit
GTCCTAAAGTGTGTATTTCTCATTT42470.3125197762396667No Hit
CTTTAGGACGTGAAATATGGCGAGG34650.25497551793511775No Hit
GTATCAACGCAGAGTACTTTTTTTT30810.22671849083927786No Hit
GTCCTACAGTGTGCATTTCTCATTT26650.1961067114854513No Hit
GGTATCAACGCAGAGTACTTTTTTT18900.13907755523733695No Hit
CTGAAGGACCTGGAATATGGCGAGA18270.13444163672942572No Hit
CTGTAGGACCTGGAATATGGCGAGA18240.13422087870523947No Hit
TATCAACGCAGAGTACTTTTTTTTT18000.13245481451174948No Hit
GTCCTTCAGTGTGCATTTCTCATTT15450.1136903824559183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG607.762501E-716.8278271
ACGGCGA509.23815E-515.095963517
TAGGACC7300.014.6032144
TCCAACG2000.013.6812218
CCAACGT1257.2759576E-1213.58686719
TACGGCG500.00157899913.20896816
GGGACCG500.001580312613.2075115
AAATGTC5350.012.1671317
AATGGCG701.1629009E-412.13068516
ATCTTAG751.1759681E-412.1160351
ATAGGAC2450.011.9368153
TTAGGCA908.060786E-611.5303654
TAGAAAT5500.011.4922494
GTCCTAT2150.011.270731
TCCTATA1801.0913936E-1111.2185512
CGGTTTC1605.0931703E-1111.20362413
GGCGAGG10050.011.17217419
GTCCTAA13500.011.143761
GTCCTAC25300.011.0544851
GGTATCA8500.011.0469731
Produced by FastQC (version 0.11.5)