FastQCFastQC Report
Thu 26 May 2016
SRR1512157_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512157_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358954
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA48080.35380152676249527No Hit
GTCCTACAGTGGACATTTCTAAATT42840.31524245853796373No Hit
GTATCAACGCAGAGTACTTTTTTTT40780.30008374087717465No Hit
CTTTAGGACGTGAAATATGGCGAGG35350.2601265384994636No Hit
GTCCTAAAGTGTGTATTTCTCATTT32860.24180362249200488No Hit
GGTATCAACGCAGAGTACTTTTTTT29610.2178881698718279No Hit
TATCAACGCAGAGTACTTTTTTTTT29240.21516548757353082No Hit
CTGAAGGACCTGGAATATGGCGAGA21390.15740047124479561No Hit
CTGTAGGACCTGGAATATGGCGAGA20040.14746636015641443No Hit
GTCCTACAGTGTGCATTTCTCATTT19950.14680408608385567No Hit
ATTTAGAAATGTCCACTGTAGGACG15660.11523568862522204No Hit
TTTCTAAATTTTCCACCTTTTTCAG15080.11096770015762124No Hit
GAATATGGCAAGAAAACTGAAAATC14300.10522799152877875No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14110.10382985737559917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7550.014.9742064
AATCCCG2350.013.7427919
ACACCGT550.003073805212.0879136
CCACCTT5500.011.91522813
ACCTTTT5950.011.65257315
GGACCTG15850.011.4451126
AGGACCT16200.011.4357185
TAACCAG1001.9277231E-611.4005295
TATAGGA2100.011.310052
TGTAGGA26100.011.1384492
CACCTTT5800.011.13517114
ACCGTGC600.00588586411.0805868
GTAGGAC26000.010.9985883
TAGACAG2600.010.9620495
AAATCCC3300.010.93668218
GATATAC6100.010.9363581
CTGTAGG25900.010.9285171
TAATACC1053.4626319E-610.8576484
AATGGCG700.001494280310.85445216
GGTATCA13350.010.8509271
Produced by FastQC (version 0.11.5)