FastQCFastQC Report
Thu 26 May 2016
SRR1512156_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512156_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895138
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37980.4242921203211125No Hit
GTCCTACAGTGGACATTTCTAAATT35600.39770404116460256No Hit
CTGTAGGACGTGGAATATGGCAAGA29050.32453096617504784No Hit
GTCCTAAAGTGTGTATTTCTCATTT27670.3091143488490043No Hit
CTTTAGGACGTGAAATATGGCGAGG23640.26409335767222486No Hit
TATCAACGCAGAGTACTTTTTTTTT23120.25828419752038234No Hit
GGTATCAACGCAGAGTACTTTTTTT22730.2539273274065004No Hit
CTGAAGGACCTGGAATATGGCGAGA13500.15081473471129592No Hit
CTGTAGGACCTGGAATATGGCGAGA12930.14444700146793008No Hit
GTCCTACAGTGTGCATTTCTCATTT12820.14321814066657879No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11410.12746637948562123No Hit
GGAATATGGCGAGAAAACTGAAAAT10160.11350205219753827No Hit
GTCCTTCAGTGTGCATTTCTCATTT9650.10780460666400041No Hit
GATATACACTGTTCTACAAATCCCG9430.10534688506129782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1850.015.30339118
CCCGTTT1055.456968E-1215.279112
TCCCGTT1004.5474735E-1115.0976611
CCAACGA1102.055458E-1013.72667819
CGTTTCC1205.820766E-1113.36921214
CCGTTTC1205.820766E-1113.36921213
CGGTGGA500.001576267513.2111899
ACGGTGG500.001576267513.2111898
CCAACGT1052.1824235E-812.58278819
TAGGACC4200.012.3574064
ATCCCGT1155.866241E-912.30856110
CGTACAC550.003224460212.0101733
GGCCTAC852.9217414E-511.851321
GTATTGG600.003791892211.7525591
GGTATCA7100.011.6343251
GTGCAGG1154.126705E-711.3875721
AATCCCG2600.011.25191719
CGGTTTC1701.2732926E-1111.10246113
GTCTAAG1001.2519515E-511.0809841
TGTAGAA4150.010.9232052
Produced by FastQC (version 0.11.5)