##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512156_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 895138 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.909912214652937 31.0 30.0 34.0 25.0 34.0 2 27.340925086411257 31.0 28.0 34.0 7.0 34.0 3 28.84352245128684 31.0 28.0 34.0 16.0 34.0 4 33.28761375341009 35.0 33.0 37.0 28.0 37.0 5 33.656493188759725 37.0 35.0 37.0 30.0 37.0 6 33.85541782384392 37.0 35.0 37.0 30.0 37.0 7 33.869484928580846 37.0 35.0 37.0 30.0 37.0 8 33.86872080059164 37.0 35.0 37.0 30.0 37.0 9 35.45746130764195 39.0 37.0 39.0 30.0 39.0 10 35.34044694784491 39.0 37.0 39.0 30.0 39.0 11 35.35476094188829 39.0 37.0 39.0 30.0 39.0 12 35.257286585978925 39.0 37.0 39.0 30.0 39.0 13 35.26149375850427 39.0 37.0 39.0 30.0 39.0 14 36.41338765642839 40.0 37.0 41.0 30.0 41.0 15 36.36456948537544 40.0 37.0 41.0 30.0 41.0 16 36.32988768212276 40.0 37.0 41.0 30.0 41.0 17 36.33039039790513 40.0 37.0 41.0 30.0 41.0 18 36.29281742033072 40.0 37.0 41.0 29.0 41.0 19 36.330073128389145 40.0 37.0 41.0 29.0 41.0 20 36.270815226255614 40.0 37.0 41.0 29.0 41.0 21 36.19931116766353 40.0 37.0 41.0 29.0 41.0 22 36.11287756748121 40.0 37.0 41.0 29.0 41.0 23 36.005579028038134 40.0 37.0 41.0 28.0 41.0 24 35.93249532474322 40.0 37.0 41.0 28.0 41.0 25 35.82271783791996 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2828625067105932 1101 2 3.238057263893328 1101 3 1.7290171117305384 1101 4 0.504746843357708 1101 5 0.1335380321963342 1101 6 0.06265722942760021 1101 7 -0.021322688868380624 1101 8 -0.1110849631032167 1101 9 -0.20879208164686958 1101 10 -0.14526556321976614 1101 11 -0.219835285469415 1101 12 -0.051672394137554534 1101 13 -0.06713525473593762 1101 14 0.0018114305326975 1101 15 -0.1017270176948557 1101 16 0.17710116648251528 1101 17 -0.057449329191399556 1101 18 0.167681986315813 1101 19 -0.03807729492474721 1101 20 0.2854910828511521 1101 21 0.11507838181830721 1101 22 0.1068175699801941 1101 23 0.029800471100145387 1101 24 -0.0013077566799637452 1101 25 0.12855424574475194 1102 1 1.1691664414300185 1102 2 4.071837724434353 1102 3 2.3217121709748945 1102 4 1.4162715471359277 1102 5 0.9750314370700153 1102 6 1.1061543231007462 1102 7 0.933950617637322 1102 8 0.8421064073416744 1102 9 0.9734681463880079 1102 10 0.9516213135966893 1102 11 0.8783231093439099 1102 12 0.7790480395758337 1102 13 0.933451599724151 1102 14 0.9689475807674413 1102 15 1.0625503129557146 1102 16 0.9373152286331958 1102 17 1.0012361168179922 1102 18 0.9554782080909021 1102 19 0.997902472569109 1102 20 1.008356050434429 1102 21 1.112074245891634 1102 22 1.0102660385695046 1102 23 1.0289201894100017 1102 24 0.9331042710419197 1102 25 1.059109801300309 1103 1 0.9815792245805355 1103 2 4.0092586207801695 1103 3 2.3728983201278773 1103 4 1.341792414274245 1103 5 0.9448219543673488 1103 6 0.6633036707998485 1103 7 0.611789830332846 1103 8 0.7123277353094792 1103 9 0.7221314974621933 1103 10 0.6949482104617459 1103 11 0.6779981758721121 1103 12 0.7497228951609571 1103 13 0.8449711200720458 1103 14 1.0471263306863037 1103 15 0.8495018609533815 1103 16 1.0313958413417055 1103 17 1.0601581976903205 1103 18 0.8045707471965073 1103 19 0.990308521767524 1103 20 0.942205112549054 1103 21 0.8393167330727849 1103 22 1.03455694530021 1103 23 1.0368569227130493 1103 24 1.04757537184026 1103 25 1.1191616446182806 1104 1 1.0440268303749143 1104 2 3.964503490339556 1104 3 2.4801597378731657 1104 4 1.3818299704408332 1104 5 1.135650034308334 1104 6 0.8868969911673616 1104 7 0.8026241975785098 1104 8 0.7248017477834949 1104 9 0.8401882173334272 1104 10 0.7365324435782412 1104 11 0.7561221279880002 1104 12 0.9688103138451538 1104 13 1.019353706753023 1104 14 0.97815040687167 1104 15 0.8031895747217419 1104 16 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-0.8986169584100949 2315 16 -0.6483554217752783 2315 17 -0.872041029325267 2315 18 -0.7794278817277913 2315 19 -0.8419236326566235 2315 20 -0.8139782229434545 2315 21 -0.7748981911401955 2315 22 -0.7783182112399274 2315 23 -0.7149284445625028 2315 24 -0.9262216981601696 2315 25 -0.8096031457258874 2316 1 -1.0674247474945737 2316 2 0.37724229113805663 2316 3 0.1734319423393984 2316 4 -0.4455611405323694 2316 5 -0.5796223563371896 2316 6 -0.9538524839104738 2316 7 -0.8576322126779061 2316 8 -0.9282195283721109 2316 9 -0.8386783645182305 2316 10 -1.0681215215234872 2316 11 -1.624937749340532 2316 12 -1.3709539624862899 2316 13 -1.1889369604928675 2316 14 -1.5029860108319113 2316 15 -1.5564622700047366 2316 16 -1.3336338489166764 2316 17 -1.365907040634184 2316 18 -1.5538739246057744 2316 19 -1.4581347457657756 2316 20 -1.5476941813042586 2316 21 -1.4434829780490261 2316 22 -1.493247580458096 2316 23 -1.3877990313874164 2316 24 -1.093317943839402 2316 25 -1.1917786137672124 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33617.0 3 0.0 4 278.0 5 1390.0 6 1593.0 7 1322.0 8 1303.0 9 1182.0 10 1169.0 11 1095.0 12 995.0 13 1015.0 14 974.0 15 1029.0 16 995.0 17 1189.0 18 1290.0 19 1614.0 20 2078.0 21 2564.0 22 3088.0 23 3603.0 24 4120.0 25 4808.0 26 5350.0 27 6221.0 28 7146.0 29 8831.0 30 11228.0 31 15194.0 32 20474.0 33 29180.0 34 42783.0 35 66284.0 36 104744.0 37 145276.0 38 360116.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.997700913155285 19.247646731565414 13.93237690724782 25.82227544803148 2 14.352584521848849 20.035157559054085 43.294124942904745 22.318132976192324 3 18.381858439704267 24.141305586401202 30.247291479079202 27.229544494815322 4 11.411759974439695 17.146629905109602 39.23082251004873 32.21078761040197 5 12.091655141441878 37.84198637528515 36.930395089919095 13.135963393353874 6 29.720958602938545 38.319205126371855 18.751675723018625 13.20816054767097 7 26.49022530182132 31.622919427170732 23.122317236941186 18.76453803406676 8 22.849884598799292 39.52206251996898 21.11741429813057 16.51063858310115 9 24.84287338935449 15.922349403108793 21.623816662905607 37.610960544631105 10 14.450963918953944 29.252393039405828 35.04182791566491 21.254815125975316 11 32.37818604313412 22.379694229233117 26.567647732414272 18.674471995218493 12 23.106458802303564 24.18450848196082 33.287269518005665 19.421763197729952 13 28.27139502512462 22.58802553349316 26.84781564406829 22.292763797313935 14 19.048837271654808 23.722093625996497 28.448526257462554 28.780542844886142 15 21.08926221431779 33.51002862128521 24.19727461017184 21.203434554225158 16 19.555532219613063 28.50856515978542 31.09565229048482 20.840250330116696 17 19.439280734994462 29.67124548671934 30.15240142280056 20.737072355485648 18 19.050034524291025 27.561668875178487 33.72978541309599 19.6585111874345 19 21.95168818749148 26.750917772499673 29.24493521408733 22.052458825921516 20 21.8584173613454 27.979708156731142 30.58947335494639 19.57240112697707 21 20.454847615100064 27.262479205513763 29.031393110475285 23.251280068910887 22 21.116227732085683 29.43475861390961 29.504581498387928 19.944432155616777 23 19.758488862871097 28.886041331048336 30.335242214958097 21.020227591122474 24 21.661873574082886 26.876512505614286 31.414308666224226 20.047305254078598 25 20.55017048983988 28.106020995052887 30.935079792820208 20.408728722287023 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 181.0 1 181.0 2 285.5 3 390.0 4 390.0 5 390.0 6 963.5 7 1537.0 8 1537.0 9 1537.0 10 2118.5 11 2700.0 12 2700.0 13 2700.0 14 3986.5 15 5273.0 16 5273.0 17 5273.0 18 9212.5 19 13152.0 20 13152.0 21 13152.0 22 20763.5 23 28375.0 24 28375.0 25 28375.0 26 41605.5 27 54836.0 28 54836.0 29 54836.0 30 71310.0 31 87784.0 32 87784.0 33 87784.0 34 95495.5 35 103207.0 36 103207.0 37 103207.0 38 106060.0 39 108913.0 40 108913.0 41 108913.0 42 111780.5 43 114648.0 44 114648.0 45 114648.0 46 113835.5 47 113023.0 48 113023.0 49 113023.0 50 104229.0 51 95435.0 52 95435.0 53 95435.0 54 81396.5 55 67358.0 56 67358.0 57 67358.0 58 56275.5 59 45193.0 60 45193.0 61 45193.0 62 36499.5 63 27806.0 64 27806.0 65 27806.0 66 21231.5 67 14657.0 68 14657.0 69 14657.0 70 10749.0 71 6841.0 72 6841.0 73 6841.0 74 4668.5 75 2496.0 76 2496.0 77 2496.0 78 1714.0 79 932.0 80 932.0 81 932.0 82 601.5 83 271.0 84 271.0 85 271.0 86 178.5 87 86.0 88 86.0 89 86.0 90 56.0 91 26.0 92 26.0 93 26.0 94 21.5 95 17.0 96 17.0 97 17.0 98 9.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 6.326063690738187 3 0.0 4 0.0 5 0.0 6 2.2342923660932727E-4 7 0.008043452517935782 8 0.0 9 0.0 10 0.004692013968795873 11 0.0025694362210072637 12 3.3514385491399093E-4 13 0.0 14 2.2342923660932727E-4 15 0.0 16 0.0 17 2.2342923660932727E-4 18 0.01295889572334098 19 0.004021726258967891 20 0.0 21 0.007708308663021792 22 0.0017874338928746182 23 0.0026811508393119275 24 0.011171461830466363 25 0.007820023281326454 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 895138.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.31650541357706 #Duplication Level Percentage of deduplicated Percentage of total 1 90.8912048567433 69.36499127496192 2 6.291019332989191 9.602172219659751 3 1.3364171417341373 3.059720580958514 4 0.4951140219925453 1.5114148775892797 5 0.2575097713212038 0.9826122928541822 6 0.170453112188452 0.7805031515454633 7 0.10137106770419585 0.5415399946059144 8 0.07338200790928032 0.4480206723094197 9 0.055813659607618644 0.3833553110036738 >10 0.2757896808327925 3.8500296701470837 >50 0.02514651985023519 1.3552448675212123 >100 0.02172060442601538 3.386923036992113 >500 0.0032729676301429833 1.77458367847206 >1k 0.001785255070987082 2.9588883713794214 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3798 0.4242921203211125 No Hit GTCCTACAGTGGACATTTCTAAATT 3560 0.39770404116460256 No Hit CTGTAGGACGTGGAATATGGCAAGA 2905 0.32453096617504784 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2767 0.3091143488490043 No Hit CTTTAGGACGTGAAATATGGCGAGG 2364 0.26409335767222486 No Hit TATCAACGCAGAGTACTTTTTTTTT 2312 0.25828419752038234 No Hit GGTATCAACGCAGAGTACTTTTTTT 2273 0.2539273274065004 No Hit CTGAAGGACCTGGAATATGGCGAGA 1350 0.15081473471129592 No Hit CTGTAGGACCTGGAATATGGCGAGA 1293 0.14444700146793008 No Hit GTCCTACAGTGTGCATTTCTCATTT 1282 0.14321814066657879 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1141 0.12746637948562123 No Hit GGAATATGGCGAGAAAACTGAAAAT 1016 0.11350205219753827 No Hit GTCCTTCAGTGTGCATTTCTCATTT 965 0.10780460666400041 No Hit GATATACACTGTTCTACAAATCCCG 943 0.10534688506129782 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1171461830466363E-4 0.0 10 0.0 0.0 0.0 1.1171461830466363E-4 0.0 11 0.0 0.0 0.0 1.1171461830466363E-4 0.0 12 0.0 1.1171461830466363E-4 0.0 1.1171461830466363E-4 0.0 13 0.0 1.1171461830466363E-4 0.0 1.1171461830466363E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 185 0.0 15.303391 18 CCCGTTT 105 5.456968E-12 15.2791 12 TCCCGTT 100 4.5474735E-11 15.09766 11 CCAACGA 110 2.055458E-10 13.726678 19 CGTTTCC 120 5.820766E-11 13.369212 14 CCGTTTC 120 5.820766E-11 13.369212 13 CGGTGGA 50 0.0015762675 13.211189 9 ACGGTGG 50 0.0015762675 13.211189 8 CCAACGT 105 2.1824235E-8 12.582788 19 TAGGACC 420 0.0 12.357406 4 ATCCCGT 115 5.866241E-9 12.308561 10 CGTACAC 55 0.0032244602 12.010173 3 GGCCTAC 85 2.9217414E-5 11.85132 1 GTATTGG 60 0.0037918922 11.752559 1 GGTATCA 710 0.0 11.634325 1 GTGCAGG 115 4.126705E-7 11.387572 1 AATCCCG 260 0.0 11.251917 19 CGGTTTC 170 1.2732926E-11 11.102461 13 GTCTAAG 100 1.2519515E-5 11.080984 1 TGTAGAA 415 0.0 10.923205 2 >>END_MODULE