FastQCFastQC Report
Thu 26 May 2016
SRR1512156_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512156_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895138
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63600.7105049724176608No Hit
TATCAACGCAGAGTACTTTTTTTTT51040.5701914118270033No Hit
GGTATCAACGCAGAGTACTTTTTTT46150.5155629634760227No Hit
CTGTAGGACGTGGAATATGGCAAGA27410.30620976877308304No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24650.2753765341209959No Hit
GTCCTACAGTGGACATTTCTAAATT22650.2530336104600631No Hit
GTCCTAAAGTGTGTATTTCTCATTT19660.21963093958696872No Hit
CTTTAGGACGTGAAATATGGCGAGG19440.21717321798426611No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14850.1658962081824255No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14090.15740589719127107No Hit
CTGAAGGACCTGGAATATGGCGAGA13120.1465695792157187No Hit
TTTCTAAATTTTCCACCTTTTTCAG11570.12925381337849584No Hit
TTGTAGAACAGTGTATATCAATGAG10730.11986978544090408No Hit
CTGTAGGACCTGGAATATGGCGAGA10540.11774720769311547No Hit
ATTTAGAAATGTCCACTGTAGGACG9950.11115604521314032No Hit
GGAATATGGCGAGAAAACTGAAAAT9880.11037404288500767No Hit
GTCCTACAGTGTGCATTTCTCATTT9680.1081397505189144No Hit
GAATATGGCAAGAAAACTGAAAATC9340.10434145349655582No Hit
GTGGTATCAACGCAGAGTACATGGG9170.10244230498537657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12900.013.4477091
TCCAACG957.398012E-812.99663618
TAGGACC3250.012.8618044
ACTGTTC3400.012.8496178
CCCGTTT604.09795E-412.66338912
ATCCCGT604.09795E-412.66338910
GTGCTAG550.002991306412.131151
CCGTTTC550.003072646412.08778113
TAGGGTG658.0162013E-411.6925495
TGGACTG1406.7848305E-1011.5359525
TTGGACT855.3156626E-511.1767014
AATCCCG1701.0913936E-1111.17607719
GACTGTT600.00588368211.0804657
AATGTCC3750.010.8905148
GGACGGG1053.4709938E-610.8543356
AAATGTC3500.010.8543347
CGGGGAA700.001493552710.8543339
GTGTTGG1158.430088E-710.7748731
CCAACGA803.7685357E-410.68712219
ATGGACT1252.234101E-710.640224
Produced by FastQC (version 0.11.5)