##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512156_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 895138 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.72466926887251 31.0 28.0 31.0 16.0 34.0 2 27.924232911573412 31.0 28.0 31.0 16.0 34.0 3 27.871420942916064 31.0 27.0 31.0 16.0 34.0 4 31.302128833766414 35.0 32.0 37.0 19.0 37.0 5 30.661429857742604 35.0 30.0 37.0 16.0 37.0 6 30.657491917447366 35.0 30.0 37.0 11.0 37.0 7 30.343465476831504 35.0 30.0 37.0 10.0 37.0 8 30.161467840712827 35.0 30.0 37.0 10.0 37.0 9 31.13378272400457 35.0 30.0 39.0 8.0 39.0 10 30.756641992631305 35.0 30.0 39.0 7.0 39.0 11 30.82971675875675 35.0 30.0 39.0 2.0 39.0 12 30.476375709667114 35.0 29.0 39.0 2.0 39.0 13 30.46878916993804 35.0 30.0 39.0 2.0 39.0 14 30.824831478498286 36.0 29.0 39.0 2.0 40.0 15 30.736240669036505 36.0 29.0 39.0 2.0 40.0 16 30.537219959380565 36.0 28.0 39.0 2.0 40.0 17 30.44091302123248 36.0 27.0 39.0 2.0 40.0 18 30.280979022228976 36.0 27.0 39.0 2.0 40.0 19 30.330059722634946 36.0 27.0 39.0 2.0 40.0 20 30.138017825184498 36.0 27.0 39.0 2.0 40.0 21 29.929577338913106 36.0 27.0 39.0 2.0 40.0 22 29.970498403598103 36.0 27.0 39.0 2.0 40.0 23 29.725280347834637 36.0 27.0 39.0 2.0 40.0 24 29.43727894469903 36.0 27.0 39.0 2.0 40.0 25 28.846479537233364 35.0 26.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32167308611079193 1101 2 0.24388642812331796 1101 3 0.25692948383903413 1101 4 0.512412889209056 1101 5 0.2767042584928099 1101 6 0.09462496972396295 1101 7 -0.682188329439505 1101 8 -0.3634250762340159 1101 9 -0.18549008580038517 1101 10 -0.1339830130463362 1101 11 -0.0686271216270562 1101 12 0.059697972810091926 1101 13 0.15415231324944756 1101 14 -0.19378431270351015 1101 15 0.020772361341812484 1101 16 -0.2207426413901281 1101 17 -0.31244355214842656 1101 18 -0.60367942468989 1101 19 -0.5715157139136551 1101 20 -0.37516988748038926 1101 21 -0.5759086159136686 1101 22 -0.32905275586767146 1101 23 -0.2903922541301007 1101 24 -0.5992403367892045 1101 25 -0.41562037583321754 1102 1 0.43012937942624063 1102 2 0.3331581696837915 1102 3 0.28203847109590896 1102 4 0.47376433119430317 1102 5 0.3662275091076097 1102 6 0.5648770376872996 1102 7 0.4090815118303368 1102 8 0.4704531933836904 1102 9 0.6765013070008656 1102 10 0.5390691890551622 1102 11 0.8678388967051625 1102 12 1.1388674340218081 1102 13 0.7483424540945194 1102 14 0.8027085332611676 1102 15 1.127468091276981 1102 16 0.9877583646460906 1102 17 1.2669354999418907 1102 18 1.238275080116729 1102 19 1.184506530664926 1102 20 0.8506572978538358 1102 21 1.307125701099519 1102 22 1.0418672083621487 1102 23 1.366521450318814 1102 24 1.6057674879369301 1102 25 1.0673887902745385 1103 1 0.11983616854806911 1103 2 0.033184943228285135 1103 3 0.10984807062859048 1103 4 0.3461782501921604 1103 5 0.10402120677749949 1103 6 0.45528805216124013 1103 7 0.35695017542480656 1103 8 0.45742745832819764 1103 9 0.5246993663245512 1103 10 0.4446620202357927 1103 11 0.08168649844462905 1103 12 0.005774521709220437 1103 13 0.21420415656742264 1103 14 0.4377121030670992 1103 15 0.16225341612932098 1103 16 0.5883011374117153 1103 17 -0.13110394641365986 1103 18 0.2985703192937237 1103 19 0.5380592988871626 1103 20 0.012614930747975706 1103 21 0.3193806826014338 1103 22 0.06474844740933605 1103 23 0.7051114581074813 1103 24 0.06424212608841984 1103 25 0.1173417337417959 1104 1 0.34803598747369335 1104 2 0.4431633149002039 1104 3 0.35375776816731985 1104 4 0.8281860799822489 1104 5 0.8654599097484592 1104 6 0.5353589729579653 1104 7 0.35562145837028325 1104 8 0.429138778829838 1104 9 0.6511370633267646 1104 10 0.4710540294907055 1104 11 0.5239606584607266 1104 12 0.7277960159081367 1104 13 0.958484805081941 1104 14 0.9490460176268201 1104 15 1.267917858487312 1104 16 1.2900607444132604 1104 17 1.3556235534186776 1104 18 1.2767428932324272 1104 19 1.0208781909802482 1104 20 1.424037286726783 1104 21 1.1974698449647896 1104 22 0.9526479008329822 1104 23 0.940430601692757 1104 24 1.0017232641743945 1104 25 0.8832444230315808 1105 1 -0.08252847726162571 1105 2 -0.20876472446501992 1105 3 0.11310021846454532 1105 4 0.20771678966371 1105 5 0.13512632916362222 1105 6 0.0240289339897366 1105 7 0.11496808756615806 1105 8 -0.019535987935380916 1105 9 0.13709805330785585 1105 10 -0.16261326749317462 1105 11 0.2562123535264895 1105 12 0.056972041566574205 1105 13 0.19852596723365679 1105 14 0.21832484419207887 1105 15 0.35987661275762406 1105 16 0.2577390527950847 1105 17 -0.1738408162466989 1105 18 0.2428917247078921 1105 19 0.285650075350123 1105 20 0.38148042758701095 1105 21 0.7040317605719331 1105 22 0.5867424902039566 1105 23 1.020852461511602 1105 24 0.7073720904262366 1105 25 0.6585680264958889 1106 1 0.0025712247427449597 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0.8028130275351621 1107 14 1.125312909518712 1107 15 1.0083219645164156 1107 16 0.8810430728955865 1107 17 1.0695008922960163 1107 18 1.494336448325985 1107 19 1.3067084924355505 1107 20 0.6280047156942139 1107 21 0.7619882094831567 1107 22 0.7438143076243904 1107 23 0.5498359204730754 1107 24 0.3929719647980967 1107 25 0.25843716384932236 1108 1 -0.24238076094941619 1108 2 -0.18340888908451447 1108 3 -0.08193647081429845 1108 4 -0.007060170428371038 1108 5 -0.4341655171304133 1108 6 -0.4920296196480187 1108 7 -0.5275190032431354 1108 8 -0.27450862418129773 1108 9 -0.08912105922192026 1108 10 -0.2765445357112526 1108 11 -0.3622894875985416 1108 12 -0.39794943751494216 1108 13 -0.32330375401308586 1108 14 -0.35365381900727044 1108 15 -0.051291635695672966 1108 16 -0.15346116888596129 1108 17 -0.0022117270040880044 1108 18 -0.025003424420571463 1108 19 -0.135248692081948 1108 20 0.05572652552100621 1108 21 0.1363961274753791 1108 22 0.2171628848601621 1108 23 0.11304619795628312 1108 24 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-0.08547063154352585 2315 6 -0.1091066233148581 2315 7 -0.33016117319548144 2315 8 0.015555130306431408 2315 9 -0.36306945087974896 2315 10 0.08791648972665911 2315 11 0.12406029470493962 2315 12 0.09739638550850316 2315 13 0.07183494855256711 2315 14 -0.22666583880792857 2315 15 0.04998444778281552 2315 16 -0.32255465133466643 2315 17 -0.38983645710157333 2315 18 -0.07043932141966636 2315 19 -0.549821795382389 2315 20 -0.9482001035828951 2315 21 -0.8021349498207258 2315 22 -0.25568786774374885 2315 23 -0.25551464846936156 2315 24 0.06914750027333127 2315 25 -0.2541172203541606 2316 1 -1.7253832986321598 2316 2 -1.5816140456980499 2316 3 -1.4296821010934444 2316 4 -1.4363255613950656 2316 5 -1.501088326202762 2316 6 -1.929732410043865 2316 7 -1.783526874807901 2316 8 -1.5762449056583208 2316 9 -1.8779948240140776 2316 10 -2.146169106389145 2316 11 -1.9804223123401563 2316 12 -2.264958506697134 2316 13 -2.4023953057981693 2316 14 -2.451350647695218 2316 15 -2.3723216922 2316 16 -2.458571354965109 2316 17 -1.975897117654231 2316 18 -2.2315963874949105 2316 19 -2.0022933732360926 2316 20 -2.005204239529668 2316 21 -2.2526884074322666 2316 22 -2.55100845356964 2316 23 -1.9149184327607571 2316 24 -2.088697811321307 2316 25 -1.83738062222033 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56250.0 3 5.0 4 828.0 5 6339.0 6 7381.0 7 6108.0 8 4940.0 9 4506.0 10 4213.0 11 3881.0 12 3728.0 13 3555.0 14 3490.0 15 3430.0 16 3491.0 17 3746.0 18 3880.0 19 4529.0 20 5379.0 21 7056.0 22 8437.0 23 10567.0 24 12525.0 25 14433.0 26 16257.0 27 18582.0 28 21090.0 29 25467.0 30 31318.0 31 38034.0 32 46898.0 33 56650.0 34 69698.0 35 85451.0 36 103137.0 37 117835.0 38 82024.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.6838065175673 24.290784116792512 16.758882292806284 19.266527072833906 2 17.17120712113663 24.8933683968282 39.5294356847771 18.405988797258075 3 21.64593615732993 27.904859362467015 30.859040728915545 19.590163751287513 4 16.531305787487515 21.136294068624057 36.72562219456665 25.60677794932178 5 12.942516071430568 40.36490779530031 35.18260475064 11.50997138262911 6 30.428939448442588 40.41566775178799 17.209748664451737 11.945644135317684 7 26.999635810344323 33.80473178437291 22.45039312374181 16.745239281540947 8 24.768694882800194 40.61317919695064 19.814263275606667 14.803862644642502 9 26.668290252452692 16.313238852556815 21.344753546380556 35.673717348609934 10 15.84057430250978 32.12476735430738 33.27274677200611 18.761911571176736 11 34.07865602845595 23.22178256313552 25.41060707957879 17.288954328829746 12 24.0023326012302 26.62170525661965 32.134039667626666 17.24192247452348 13 30.269634402740138 23.433034906349636 26.21517576060898 20.08215493030125 14 20.73713773742149 25.26258521032511 28.103487953812706 25.896789098440685 15 22.783079257053103 34.78737356698073 23.477832468289805 18.95171470767636 16 20.806624230006992 30.44234520263915 29.571641467572597 19.179389099781265 17 20.86449240228881 31.318746383239233 28.644074991788976 19.172686222682984 18 20.35663774747581 28.593356555078657 32.93302261774162 18.11698307970391 19 23.447892950584155 27.610379628615924 29.757087733958336 19.18463968684158 20 23.304563095299272 28.745400150591305 30.379226443297014 17.57081031081241 21 22.387832937491204 27.72097710073754 29.288444910170274 20.602745051600984 22 22.66857177329082 29.652411136606872 29.920637935156368 17.758379154945942 23 21.479593090674285 29.580690351655274 30.23980659965279 18.699909958017646 24 22.766210349689096 28.131863466862093 30.974888788097477 18.12703739535133 25 22.095095304223864 29.088205424983883 30.638903071342146 18.177796199450107 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 256.0 1 256.0 2 432.5 3 609.0 4 609.0 5 609.0 6 1413.0 7 2217.0 8 2217.0 9 2217.0 10 2992.0 11 3767.0 12 3767.0 13 3767.0 14 5119.5 15 6472.0 16 6472.0 17 6472.0 18 11140.0 19 15808.0 20 15808.0 21 15808.0 22 24984.0 23 34160.0 24 34160.0 25 34160.0 26 49069.5 27 63979.0 28 63979.0 29 63979.0 30 79484.5 31 94990.0 32 94990.0 33 94990.0 34 101674.0 35 108358.0 36 108358.0 37 108358.0 38 108964.5 39 109571.0 40 109571.0 41 109571.0 42 110635.5 43 111700.0 44 111700.0 45 111700.0 46 108219.0 47 104738.0 48 104738.0 49 104738.0 50 97005.0 51 89272.0 52 89272.0 53 89272.0 54 75437.0 55 61602.0 56 61602.0 57 61602.0 58 50862.5 59 40123.0 60 40123.0 61 40123.0 62 32176.0 63 24229.0 64 24229.0 65 24229.0 66 18786.5 67 13344.0 68 13344.0 69 13344.0 70 9783.0 71 6222.0 72 6222.0 73 6222.0 74 4346.5 75 2471.0 76 2471.0 77 2471.0 78 1666.5 79 862.0 80 862.0 81 862.0 82 578.0 83 294.0 84 294.0 85 294.0 86 183.0 87 72.0 88 72.0 89 72.0 90 44.5 91 17.0 92 17.0 93 17.0 94 10.5 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33804843498991216 2 0.0 3 0.0 4 0.0 5 0.025359218355158645 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.006367733243365827 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 895138.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.44722743620024 #Duplication Level Percentage of deduplicated Percentage of total 1 92.23198794634791 70.50879759427337 2 5.263283553685059 8.047268697795479 3 1.1266474076051884 2.5838721182879767 4 0.4534742215556084 1.3866738780686179 5 0.23593242814438803 0.90181899969645 6 0.14881947087736921 0.6826101562258333 7 0.09670873273654446 0.5175180140604123 8 0.06871560912687316 0.42024942394712883 9 0.05101331938723473 0.350984414652431 >10 0.2684888241544165 3.7176148564152993 >50 0.02593689200691318 1.3893619219164624 >100 0.02345551126156169 3.677320806540746 >500 0.0034964735437613714 1.923415367099392 >1k 0.0017482367718806857 2.615711251401061 >5k 2.913727953134476E-4 1.2767824996194228 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6360 0.7105049724176608 No Hit TATCAACGCAGAGTACTTTTTTTTT 5104 0.5701914118270033 No Hit GGTATCAACGCAGAGTACTTTTTTT 4615 0.5155629634760227 No Hit CTGTAGGACGTGGAATATGGCAAGA 2741 0.30620976877308304 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2465 0.2753765341209959 No Hit GTCCTACAGTGGACATTTCTAAATT 2265 0.2530336104600631 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1966 0.21963093958696872 No Hit CTTTAGGACGTGAAATATGGCGAGG 1944 0.21717321798426611 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1485 0.1658962081824255 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1409 0.15740589719127107 No Hit CTGAAGGACCTGGAATATGGCGAGA 1312 0.1465695792157187 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1157 0.12925381337849584 No Hit TTGTAGAACAGTGTATATCAATGAG 1073 0.11986978544090408 No Hit CTGTAGGACCTGGAATATGGCGAGA 1054 0.11774720769311547 No Hit ATTTAGAAATGTCCACTGTAGGACG 995 0.11115604521314032 No Hit GGAATATGGCGAGAAAACTGAAAAT 988 0.11037404288500767 No Hit GTCCTACAGTGTGCATTTCTCATTT 968 0.1081397505189144 No Hit GAATATGGCAAGAAAACTGAAAATC 934 0.10434145349655582 No Hit GTGGTATCAACGCAGAGTACATGGG 917 0.10244230498537657 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 1.1171461830466363E-4 0.0 0.0 0.0 0.0 11 1.1171461830466363E-4 0.0 0.0 0.0 0.0 12 1.1171461830466363E-4 0.0 0.0 0.0 0.0 13 1.1171461830466363E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1290 0.0 13.447709 1 TCCAACG 95 7.398012E-8 12.996636 18 TAGGACC 325 0.0 12.861804 4 ACTGTTC 340 0.0 12.849617 8 CCCGTTT 60 4.09795E-4 12.663389 12 ATCCCGT 60 4.09795E-4 12.663389 10 GTGCTAG 55 0.0029913064 12.13115 1 CCGTTTC 55 0.0030726464 12.087781 13 TAGGGTG 65 8.0162013E-4 11.692549 5 TGGACTG 140 6.7848305E-10 11.535952 5 TTGGACT 85 5.3156626E-5 11.176701 4 AATCCCG 170 1.0913936E-11 11.176077 19 GACTGTT 60 0.005883682 11.080465 7 AATGTCC 375 0.0 10.890514 8 GGACGGG 105 3.4709938E-6 10.854335 6 AAATGTC 350 0.0 10.854334 7 CGGGGAA 70 0.0014935527 10.854333 9 GTGTTGG 115 8.430088E-7 10.774873 1 CCAACGA 80 3.7685357E-4 10.687122 19 ATGGACT 125 2.234101E-7 10.64022 4 >>END_MODULE