FastQCFastQC Report
Thu 26 May 2016
SRR1512155_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512155_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943554
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26570.28159490606790916No Hit
TATCAACGCAGAGTACTTTTTTTTT16910.17921602791149208No Hit
GGTATCAACGCAGAGTACTTTTTTT16040.1699955699408831No Hit
GTCCTAAAGTGTGTATTTCTCATTT9970.1056643286976686No Hit
CTTTAGGACGTGAAATATGGCGAGG9750.10333271863613529No Hit
GTCCTACAGTGGACATTTCTAAATT9600.10174298450327168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5250.013.2111151
AGGTTGC655.700926E-513.0903796
GTATAAT1257.239578E-1012.682671
CCTACAC803.0088306E-511.8177033
TAGGACC2450.011.5765264
GTATAAG700.001048516411.32381251
CTAGGAC1105.279562E-711.1731013
AGCACCG600.00608798411.0298575
TTATGAC700.00155181510.8047573
GTATCAA11300.010.5221271
TTCCACC2250.010.50462511
CCAGTAC909.9826444E-510.5046243
TAATCCC1106.3944226E-610.3136325
GTATTAA1251.4961151E-610.3046691
GTAGGGC750.002754215210.084443
GATATAC2301.8189894E-129.9083371
TGTAGGA6750.09.8349422
CTAAGAC1356.86774E-79.8043173
AACAGTG2700.09.8043177
TTATACT1451.7306411E-79.7801694
Produced by FastQC (version 0.11.5)