FastQCFastQC Report
Thu 26 May 2016
SRR1512155_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512155_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943554
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27370.2900734881098485No Hit
TATCAACGCAGAGTACTTTTTTTTT19190.2033799867310191No Hit
GGTATCAACGCAGAGTACTTTTTTT18340.1943714933114586No Hit
GTCCTACAGTGGACATTTCTAAATT10910.11562666259694729No Hit
GTCCTAAAGTGTGTATTTCTCATTT10490.11117540702492916No Hit
CTTTAGGACGTGAAATATGGCGAGG10210.10820790331025039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGAC851.7113052E-814.53099253
GTCTAAG801.914159E-613.1118841
GCCGGTT604.1002667E-412.66267511
GGTATCA4950.012.3292651
TCCAACG1353.7107384E-1011.9604618
CCAACGT658.032378E-411.68986219
TAGGACC2300.011.5664764
CCAACGA752.0761987E-411.39761519
TCCAATA700.001489108810.8583242
AAAGGCG1053.456389E-610.8583245
GTCTAGA1605.0749804E-1010.7279051
GTAGGGG803.763928E-410.6886633
GTATCAA11000.010.6628881
GTCCTAT909.148179E-510.5954621
AAGGCGT909.535487E-510.5522296
GTGTAGC1553.430614E-910.4587471
ATAGTAG1002.392755E-510.4511373
ATAGGAC1106.0280818E-610.3647633
TTTATAC1201.5187143E-610.2927863
GGCGAGG2800.010.17644219
Produced by FastQC (version 0.11.5)