##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512155_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 943554 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.66803807731195 31.0 31.0 34.0 27.0 34.0 2 30.86073187120186 31.0 31.0 34.0 27.0 34.0 3 30.960368987890465 31.0 31.0 34.0 27.0 34.0 4 34.556926259652336 37.0 35.0 37.0 31.0 37.0 5 34.13278731264983 37.0 35.0 37.0 30.0 37.0 6 34.06403978998552 37.0 35.0 37.0 30.0 37.0 7 33.88393986989616 37.0 35.0 37.0 29.0 37.0 8 33.787447247322355 37.0 35.0 37.0 29.0 37.0 9 35.252678702013874 39.0 35.0 39.0 29.0 39.0 10 35.04906025516293 38.0 35.0 39.0 28.0 39.0 11 35.04176655496135 38.0 35.0 39.0 28.0 39.0 12 34.85558643172516 38.0 35.0 39.0 27.0 39.0 13 34.827358052639276 38.0 35.0 39.0 27.0 39.0 14 35.80159906057311 39.0 36.0 41.0 27.0 41.0 15 35.73459070705015 39.0 36.0 41.0 27.0 41.0 16 35.63215565828771 39.0 36.0 41.0 27.0 41.0 17 35.560365384493096 39.0 35.0 41.0 27.0 41.0 18 35.49289070895784 39.0 35.0 41.0 27.0 41.0 19 35.51143654735182 39.0 35.0 41.0 27.0 41.0 20 35.397488643999175 39.0 35.0 41.0 27.0 41.0 21 35.269385747927515 39.0 35.0 40.0 27.0 41.0 22 35.14979958751698 39.0 35.0 40.0 26.0 41.0 23 35.20619911525996 39.0 35.0 41.0 27.0 41.0 24 35.089411946746026 39.0 35.0 41.0 26.0 41.0 25 34.65740381578585 39.0 34.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08190472282256422 1101 2 0.15382960205673157 1101 3 0.2031079216299183 1101 4 0.20726983901651153 1101 5 0.27539435674435 1101 6 0.3087097445102174 1101 7 0.2793335033960318 1101 8 0.20071481589764062 1101 9 0.208932302213249 1101 10 0.13531158753032457 1101 11 0.038403671726236155 1101 12 0.25531271815611234 1101 13 0.09042703176246647 1101 14 0.013469771391072527 1101 15 0.09831240141642184 1101 16 0.22605652831978063 1101 17 0.08948870324370972 1101 18 0.2767670169082308 1101 19 0.23879625134662064 1101 20 0.27545329972601706 1101 21 0.09955997689545626 1101 22 0.22503467718237147 1101 23 0.10420601110703132 1101 24 -0.3562334193669088 1101 25 -0.17807921838416974 1102 1 0.13009547087058593 1102 2 0.13154627851826461 1102 3 0.0721421476954589 1102 4 0.14342689272037035 1102 5 -0.0965699339673165 1102 6 -0.085049525632229 1102 7 0.31937911949319897 1102 8 0.14203376476843488 1102 9 0.25458478031472254 1102 10 0.3474362342154578 1102 11 0.15685720207633125 1102 12 0.23152658209141208 1102 13 0.22176780919509298 1102 14 0.22897924442720097 1102 15 0.26392961799493975 1102 16 0.14017233163883702 1102 17 0.28131138815681567 1102 18 0.34846964473125297 1102 19 0.3245335250152124 1102 20 0.22959373014047912 1102 21 0.4576659293763541 1102 22 0.3924023765079241 1102 23 0.26581432834880303 1102 24 0.45188092871470076 1102 25 0.17890312286901633 1103 1 0.36330981283079566 1103 2 0.45958187093781433 1103 3 0.5230004604677845 1103 4 0.561291347547396 1103 5 0.6553698004019424 1103 6 0.6665437338564573 1103 7 0.8183370700417854 1103 8 0.8104441800133273 1103 9 0.7091020778106554 1103 10 0.6721488175531647 1103 11 0.6132013671243612 1103 12 0.692960253150261 1103 13 0.6378473934252327 1103 14 0.941725320336694 1103 15 0.8406528055638418 1103 16 0.9046849044773069 1103 17 0.9386057343167238 1103 18 1.1830305140682285 1103 19 1.0605590381655148 1103 20 0.9463820129899716 1103 21 1.2501169766754288 1103 22 1.269422167573076 1103 23 1.1688932301284183 1103 24 1.4615376036057697 1103 25 1.2663539076871189 1104 1 0.15803298041208436 1104 2 0.12845483279031455 1104 3 0.17687473127899267 1104 4 0.03832282676952303 1104 5 0.01636579449433384 1104 6 -0.052511741295553804 1104 7 0.16141514819403113 1104 8 0.0801514178140792 1104 9 0.1618363196765884 1104 10 0.07330514673899557 1104 11 0.35275562963726514 1104 12 0.49986661567749024 1104 13 0.1702996905242884 1104 14 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2315 4 -0.656164702175623 2315 5 -0.67033466723435 2315 6 -0.7320055321497847 2315 7 -0.5610946727492205 2315 8 -0.5650223327409307 2315 9 -1.0007572854109839 2315 10 -0.6817795433413067 2315 11 -1.0056435914569022 2315 12 -1.0182660412236544 2315 13 -0.8710607664656607 2315 14 -1.1515823654930202 2315 15 -1.1826497247479395 2315 16 -1.1267278101391014 2315 17 -1.193361147128961 2315 18 -1.2411012124962468 2315 19 -1.2511875321826622 2315 20 -1.1361658398703156 2315 21 -1.1157686940656717 2315 22 -1.5128804387363743 2315 23 -1.4101648916609335 2315 24 -1.2690947997488067 2315 25 -1.2900527990049966 2316 1 -1.1678696454084836 2316 2 -1.1782793028770833 2316 3 -1.1413656042425266 2316 4 -1.2212048902253656 2316 5 -1.2902133448200317 2316 6 -1.3802433240818388 2316 7 -1.3677332836075777 2316 8 -1.4298267003478458 2316 9 -1.7237873127085308 2316 10 -1.8241529130713587 2316 11 -1.5626583017996367 2316 12 -1.5923106272109138 2316 13 -1.7165073846033607 2316 14 -2.0000711431696985 2316 15 -1.9640161184391687 2316 16 -1.9224633272758922 2316 17 -2.1714017901377574 2316 18 -1.9768791924780516 2316 19 -1.9091098858374949 2316 20 -2.0108132466037105 2316 21 -1.7508612024065897 2316 22 -2.0015898715540885 2316 23 -1.8263174545969392 2316 24 -1.852382637176774 2316 25 -1.6666201329449422 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16210.0 3 0.0 4 283.0 5 2628.0 6 3344.0 7 2633.0 8 2182.0 9 1916.0 10 1787.0 11 1712.0 12 1704.0 13 1627.0 14 1656.0 15 1513.0 16 1662.0 17 1763.0 18 1877.0 19 2123.0 20 2504.0 21 3284.0 22 4228.0 23 5387.0 24 6622.0 25 7830.0 26 9033.0 27 10329.0 28 11958.0 29 14662.0 30 18754.0 31 23781.0 32 30693.0 33 39201.0 34 51215.0 35 67573.0 36 96657.0 37 163796.0 38 329427.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.00855861512625 20.385935860405816 13.94973383366786 24.655771690800073 2 14.764708750108632 21.69255813657724 40.34109335554722 23.201639757766912 3 17.914925907791183 26.072169690340985 30.94841418721133 25.064490214656498 4 12.562609029265944 18.312041494180516 38.646118823088024 30.479230653465518 5 12.820061499310784 38.19266249602375 36.19785812745202 12.789417877213443 6 30.443938555715945 38.2901243596021 18.542764907996787 12.723172176685171 7 26.570074420753876 32.34812210005998 23.411060734202813 17.67074274498333 8 24.60316703284358 37.035906534270666 21.274083683347587 17.08684274953817 9 26.004341034005474 15.44840040951551 20.87119550126437 37.67606305521465 10 14.620784819946712 29.505147559122214 34.86869855885302 21.005369062078056 11 33.45924027665613 22.972082148981404 25.24042079202674 18.32825678233572 12 22.35155592578697 25.25716599155957 32.417752455079416 19.973525627574045 13 28.262611360876004 22.379217299698798 26.55237538074132 22.80579595868387 14 20.775917435568076 22.24006257193547 28.365520150410045 28.618499842086408 15 22.535329191546005 30.828548233593413 24.219069602799628 22.41705297206095 16 21.914061092422905 28.499799693499263 27.794487649885436 21.791651564192406 17 21.269688857235515 28.744300803133683 28.33637502464088 21.649635314989922 18 21.155651928771434 27.96109178700954 29.84015753205434 21.04309875216469 19 22.821211761513936 27.338927796242285 28.321279590314045 21.51858085192973 20 22.91771324163747 27.638057811211652 28.64139201359965 20.802836933551234 21 22.680524909014217 27.170146064772126 27.989071107747943 22.160257918465714 22 22.6686548941555 27.911492082064193 28.025211063701704 21.394641960078598 23 22.057031182105106 27.722737649355523 28.274799322561293 21.945431845978078 24 22.37625861155273 27.303550609432964 28.821303656597774 21.498887122416534 25 22.38729725375817 27.52961176161366 28.453045115013186 21.63004586961498 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 585.0 1 585.0 2 484.0 3 383.0 4 383.0 5 383.0 6 835.0 7 1287.0 8 1287.0 9 1287.0 10 1907.5 11 2528.0 12 2528.0 13 2528.0 14 4055.5 15 5583.0 16 5583.0 17 5583.0 18 9649.5 19 13716.0 20 13716.0 21 13716.0 22 21021.0 23 28326.0 24 28326.0 25 28326.0 26 39830.5 27 51335.0 28 51335.0 29 51335.0 30 64203.0 31 77071.0 32 77071.0 33 77071.0 34 85697.0 35 94323.0 36 94323.0 37 94323.0 38 101161.5 39 108000.0 40 108000.0 41 108000.0 42 112848.5 43 117697.0 44 117697.0 45 117697.0 46 118512.0 47 119327.0 48 119327.0 49 119327.0 50 113948.0 51 108569.0 52 108569.0 53 108569.0 54 97137.0 55 85705.0 56 85705.0 57 85705.0 58 72663.5 59 59622.0 60 59622.0 61 59622.0 62 48187.5 63 36753.0 64 36753.0 65 36753.0 66 28013.5 67 19274.0 68 19274.0 69 19274.0 70 13992.0 71 8710.0 72 8710.0 73 8710.0 74 5982.0 75 3254.0 76 3254.0 77 3254.0 78 2184.5 79 1115.0 80 1115.0 81 1115.0 82 704.5 83 294.0 84 294.0 85 294.0 86 188.5 87 83.0 88 83.0 89 83.0 90 47.5 91 12.0 92 12.0 93 12.0 94 7.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41502659095292904 2 0.0 3 0.0 4 0.0 5 0.048115953088005564 6 0.0 7 0.0 8 0.0022256277860090677 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 2.1196455104848265E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005723042878309032 25 0.0010598227552424133 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 943554.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.73521660724867 #Duplication Level Percentage of deduplicated Percentage of total 1 89.84362423849093 73.43388087913314 2 7.523397355233262 12.29853024904785 3 1.400455130216677 3.433995102509783 4 0.5018815072189503 1.6408557481485337 5 0.23063817031428133 0.9425630404268649 6 0.13097420341437632 0.6423122931621539 7 0.07444480424716157 0.4259333540298184 8 0.052116428226521856 0.34077980399127145 9 0.03917045581903557 0.2881445121876183 >10 0.17779347906176735 2.4935308896402537 >50 0.013726892272013295 0.7876144237526359 >100 0.01022428087396294 1.8044419158071947 >500 7.765273054903686E-4 0.4465088295729385 >1k 7.765273054903686E-4 1.0209089585900093 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2737 0.2900734881098485 No Hit TATCAACGCAGAGTACTTTTTTTTT 1919 0.2033799867310191 No Hit GGTATCAACGCAGAGTACTTTTTTT 1834 0.1943714933114586 No Hit GTCCTACAGTGGACATTTCTAAATT 1091 0.11562666259694729 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1049 0.11117540702492916 No Hit CTTTAGGACGTGAAATATGGCGAGG 1021 0.10820790331025039 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 2.1196455104848265E-4 13 0.0 0.0 0.0 0.0 2.1196455104848265E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAAGAC 85 1.7113052E-8 14.5309925 3 GTCTAAG 80 1.914159E-6 13.111884 1 GCCGGTT 60 4.1002667E-4 12.662675 11 GGTATCA 495 0.0 12.329265 1 TCCAACG 135 3.7107384E-10 11.96046 18 CCAACGT 65 8.032378E-4 11.689862 19 TAGGACC 230 0.0 11.566476 4 CCAACGA 75 2.0761987E-4 11.397615 19 TCCAATA 70 0.0014891088 10.858324 2 AAAGGCG 105 3.456389E-6 10.858324 5 GTCTAGA 160 5.0749804E-10 10.727905 1 GTAGGGG 80 3.763928E-4 10.688663 3 GTATCAA 1100 0.0 10.662888 1 GTCCTAT 90 9.148179E-5 10.595462 1 AAGGCGT 90 9.535487E-5 10.552229 6 GTGTAGC 155 3.430614E-9 10.458747 1 ATAGTAG 100 2.392755E-5 10.451137 3 ATAGGAC 110 6.0280818E-6 10.364763 3 TTTATAC 120 1.5187143E-6 10.292786 3 GGCGAGG 280 0.0 10.176442 19 >>END_MODULE