FastQCFastQC Report
Thu 26 May 2016
SRR1512154_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512154_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154981
Sequences flagged as poor quality0
Sequence length25
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA127838.248107832573025No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT90845.86136365102819No Hit
GTATCAACGCAGAGTACTTTTTTTT53863.4752647098676612No Hit
TATCAACGCAGAGTACTTTTTTTTT40882.6377426910395467No Hit
GGTATCAACGCAGAGTACTTTTTTT39012.5170827391744797No Hit
GGTATCAACGCAGAAAAAAAAAAAA34002.193817306637588No Hit
GTACATAAGCAGTGGTATCAACGCA27671.7853801433724135No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26401.7034346145656565No Hit
GGTATCAACGCAGAGAAAAAAAAAA23151.4937314896664753No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20181.302095095527839No Hit
GTATCAACGCAGAGAAAAAAAAAAA19331.2472496628618992No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18131.1698208167452784No Hit
GTATCAACGCAGAAAAAAAAAAAAA15511.0007678360573231No Hit
GCTTATGTACTCTGCGTTGATACCA11760.7588026919428833No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA11370.7336383169549816No Hit
TATCAACGCAGAGAAAAAAAAAAAA11270.7271859131119299No Hit
ANAAAAAAAAAAAAAAAAAAAAAAA9910.6394332208464263No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8100.52264471128719No Hit
CTTATGTACTCTGCGTTGATACCAC8090.5219994709028849No Hit
TATCAACGCAGAAAAAAAAAAAAAA7480.4826398074602693No Hit
GAGTACATAAGCAGTGGTATCAACG7250.46779927862125037No Hit
TNTTTTTTTTTTTTTTTTTTTTTTT6650.42908485556294No Hit
ATCAACGCAGAGTACTTTTTTTTTT6220.40133951903781756No Hit
GTGGTATCAACGCAGAGTACTTTTT5600.3613346152108968No Hit
TATGTACTCTGCGTTGATACCACTG5250.3387512017602158No Hit
GTGGTATCAACGCAGAAAAAAAAAA4900.31616778830953474No Hit
GAGAAAAAAAAAAAAAAAAAAAAAA4420.28519624986288644No Hit
CATAAGCAGTGGTATCAACGCAGAG4320.2787438460198347No Hit
GCGTTGATACCACTGCTTATGTACT4250.2742271633296985No Hit
ACGCAGAGAAAAAAAAAAAAAAAAA4220.27229144217678297No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT4100.2645485575651209No Hit
AGAAAAAAAAAAAAAAAAAAAAAAA3810.24583658642027087No Hit
GNATCAACGCAGAGTACTTTTTTTT3780.24390086526735535No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA3600.23228653834986226No Hit
GTGGTATCAACGCAGAGTACATGGG3560.22970557681264153No Hit
ACGCAGAGTACATAAGCAGTGGTAT3400.2193817306637588No Hit
GTGGTATCAACGCAGAGAAAAAAAA3390.2187364902794536No Hit
TATCAACGCAGAGTACATAAGCAGT3360.2168007691265381No Hit
GTACTGGTTCACTATCGGTCAGTCA3340.21551028835792774No Hit
CTGCTTATGTACTCTGCGTTGATAC3310.21357456720501222No Hit
GTATCAACGCAGAGTACATAAGCAG3090.19937927875029843No Hit
GGTATCAACGCAGAGTACATGGGGG2990.1929268749072467No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2930.18905543260141566No Hit
ACGCAGAAAAAAAAAAAAAAAAAAA2860.18453874991127944No Hit
GGTATCAACGCAGAGTACATAAGCA2800.1806673076054484No Hit
TATTTTTTTTTTTTTTTTTTTTTTT2730.17615062491531222No Hit
TNTCAACGCAGAGTACTTTTTTTTT2630.16969822107226046No Hit
TCTTTTTTTTTTTTTTTTTTTTTTT2600.16776249991934494No Hit
GNTATCAACGCAGAGTACTTTTTTT2580.1664720191507346No Hit
AAGCAGTGGTATCAACGCAGAGTAC2550.16453629799781908No Hit
GTATCAACGCAGAGTAAAAAAAAAA2510.1619553364605984No Hit
GNTATCAACGCAGAAAAAAAAAAAA2510.1619553364605984No Hit
CAACGCAGAGTACTTTTTTTTTTTT2490.16066485569198805No Hit
TGTTTTTTTTTTTTTTTTTTTTTTT2420.15614817300185185No Hit
GGATACCACGTGTCCCGCCCTACTC2410.15550293261754666No Hit
ATAAAAAAAAAAAAAAAAAAAAAAA2360.1522767306960208No Hit
GTGGTATCAACGCAGAGTACATAAG2280.1471148076215794No Hit
AACGCAGAGTACTTTTTTTTTTTTT2270.14646956723727425No Hit
GTACATGGGGAAAAAAAAAAAAAAA2210.14259812493144322No Hit
ACTCTGCGTTGATACCACTGCTTAT2150.13872668262561216No Hit
GGTATCAACGCAGAGTACATGGGGA2100.13550048070408632No Hit
ACATAAGCAGTGGTATCAACGCAGA2070.13356475955117078No Hit
TCAACGCAGAGTACTTTTTTTTTTT2060.13291951916686562No Hit
ATACAGGGTGACAGCCCCGTACACA2040.13162903839825527No Hit
ATCATTAACTGAATCCATAGGTTAA2030.1309837980139501No Hit
CCCATGTACTCTGCGTTGATACCAC2020.1303385576296449No Hit
GTACATGGGGGGTCTTGAGGGGTTT2020.1303385576296449No Hit
GAATCAACGCAGAGTACTTTTTTTT2000.1290480768610346No Hit
GAGTAAAAAAAAAAAAAAAAAAAAA1990.1284028364767294No Hit
GGTATCAACGCAGAGTAAAAAAAAA1870.12065995186506732No Hit
GNACTTTTTTTTTTTTTTTTTTTTT1850.11936947109645699No Hit
GNTATCAACGCAGAGAAAAAAAAAA1820.11743374994354146No Hit
GGATCAACGCAGAGTACTTTTTTTT1790.11549802879062596No Hit
TATCAACGCAGAGTAAAAAAAAAAA1710.11033610571618456No Hit
GTACATGGGAAAAAAAAAAAAAAAA1700.1096908653318794No Hit
GTATCAACGCAGAGTACATGGGGAA1680.10840038456326904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCAAC408.283774E-619.968281
CTCAACG452.2864499E-517.7495842
TTCAACG601.5865842E-514.9762112
TTTCAAC551.2725127E-414.5223871
TTTCCGA400.00545270214.16916815
TTCCGAA400.00545270214.16916816
TCCGAAT400.00545270214.16916817
GCATCAA500.001022721213.9777971
CATCAAC550.002097647212.7070882
GGATCAA801.6970247E-512.4801761
TGTTTTT853.1590014E-511.7460471
TACTGGT957.697177E-611.5605832
TGGTTCA907.819786E-611.5452485
CTGGTTC907.819786E-611.5452484
ATCTAAG709.7515364E-411.4104452
GATCAAC905.660553E-511.093492
GGTTCAC951.4254252E-510.9376026
GTACTGG1103.2690805E-610.891791
CGGTCAG1053.6402398E-610.79555616
CATCTAA750.001735235410.649751
Produced by FastQC (version 0.11.5)