##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512154_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 154981 Sequences flagged as poor quality 0 Sequence length 25 %GC 32 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.664081403526882 31.0 28.0 33.0 16.0 34.0 2 25.542885902142842 31.0 23.0 33.0 7.0 34.0 3 27.199901923461585 31.0 25.0 33.0 16.0 34.0 4 31.81958433614443 35.0 32.0 37.0 19.0 37.0 5 32.41274091662849 35.0 33.0 37.0 23.0 37.0 6 32.84028364767294 35.0 35.0 37.0 26.0 37.0 7 32.997012537020666 37.0 35.0 37.0 26.0 37.0 8 33.073267045637856 37.0 35.0 37.0 26.0 37.0 9 34.43601473729038 39.0 35.0 39.0 27.0 39.0 10 34.39098986327356 39.0 35.0 39.0 26.0 39.0 11 34.34319045560424 39.0 35.0 39.0 25.0 39.0 12 34.25870913208716 39.0 35.0 39.0 25.0 39.0 13 34.10654209225647 38.0 35.0 39.0 25.0 39.0 14 34.98357217981559 39.0 35.0 41.0 24.0 41.0 15 34.85374981449339 39.0 34.0 41.0 22.0 41.0 16 34.81433207941619 39.0 34.0 41.0 23.0 41.0 17 34.87102289958124 39.0 35.0 40.0 25.0 41.0 18 34.66109394054755 38.0 35.0 40.0 25.0 41.0 19 34.5573263819436 38.0 35.0 41.0 25.0 41.0 20 34.502713235816 38.0 35.0 41.0 25.0 41.0 21 34.45541066324259 38.0 35.0 41.0 25.0 41.0 22 34.294500616204566 38.0 35.0 41.0 24.0 41.0 23 34.048973745168766 38.0 34.0 41.0 21.0 41.0 24 33.90256870196992 38.0 34.0 40.0 21.0 41.0 25 33.75413760396436 37.0 34.0 40.0 21.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.03687605057659482 1101 2 2.5520128242365523 1101 3 1.0698412612183184 1101 4 -0.6175148291759278 1101 5 -0.6252517595258276 1101 6 -1.6317604077395345 1101 7 -1.6297830934674202 1101 8 -1.0175985892396184 1101 9 -2.1708993604397975 1101 10 -2.3418445690937517 1101 11 -1.637619380903942 1101 12 -1.259841692011939 1101 13 -1.3467293113938865 1101 14 -1.7734796077568689 1101 15 -1.7738436157993362 1101 16 -2.1155213801462054 1101 17 -2.688191517384496 1101 18 -2.5828289658973276 1101 19 -1.9316936886817189 1101 20 -2.1337701788743644 1101 21 -2.5510813939666335 1101 22 -2.0310266105937487 1101 23 -2.312011220978782 1101 24 -2.788198933334005 1101 25 -2.366074896746529 1102 1 1.2126035807050002 1102 2 4.380142470385824 1102 3 3.0616640385005063 1102 4 1.6611054503364109 1102 5 1.2614565366383133 1102 6 1.0884298985346277 1102 7 0.9939159847365673 1102 8 0.7439238609536574 1102 9 0.48119698211149853 1102 10 1.106118559065628 1102 11 0.8710038721439304 1102 12 0.7649873296948684 1102 13 0.6612992345472364 1102 14 0.9122176863257963 1102 15 0.8161058340965894 1102 16 0.7420462677871882 1102 17 0.7377674478251421 1102 18 0.7522884887562498 1102 19 0.14859177072952434 1102 20 1.1325099281729152 1102 21 0.6798136402290282 1102 22 1.180689119409223 1102 23 1.0607214581767366 1102 24 0.9957737101390833 1102 25 0.823934023860069 1103 1 0.5727730722304223 1103 2 2.973216695984892 1103 3 1.8803373289739156 1103 4 0.9035075543691384 1103 5 0.7998843566266203 1103 6 0.7000605238999071 1103 7 0.5538224721708147 1103 8 0.5507619673242061 1103 9 -0.037505531038711126 1103 10 0.00237805643558886 1103 11 0.4443527909048939 1103 12 -0.44117865510933996 1103 13 -0.109887206130729 1103 14 -0.2867887426630986 1103 15 -0.23240380938675287 1103 16 -0.6362412833524971 1103 17 -0.5189537678382123 1103 18 -0.9985881915476611 1103 19 -0.37361746363634296 1103 20 -0.11471391753134697 1103 21 -0.37760891967746346 1103 22 -0.6043780927474103 1103 23 -0.221569599684166 1103 24 -0.5747990543261423 1103 25 -0.4677082905759278 1104 1 1.415796328044376 1104 2 3.9275738197485914 1104 3 2.795667454608772 1104 4 2.2103105482210488 1104 5 1.3776042176263559 1104 6 1.40067533277454 1104 7 1.6007350623873364 1104 8 1.1566160170435325 1104 9 1.3721442952109584 1104 10 1.491284765871569 1104 11 1.6710301498998135 1104 12 0.6761028204319643 1104 13 0.6963143442568693 1104 14 1.0088541335732018 1104 15 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-1.469449679154593 2315 17 -1.0245759567437602 2315 18 -1.2099075318774979 2315 19 -1.3241422012675272 2315 20 -1.9328548470665794 2315 21 -2.1548952764990474 2315 22 -2.233238662462554 2315 23 -1.8446580554562786 2315 24 -1.9197016030517773 2315 25 -2.434724782330054 2316 1 -1.743118400637794 2316 2 -1.9910308314142 2316 3 -1.4562705298873517 2316 4 -2.3323251106936773 2316 5 -2.122395782373644 2316 6 -2.932658000558792 2316 7 -2.4419005965273968 2316 8 -1.990670804661896 2316 9 -2.333282061378192 2316 10 -2.9854594201749443 2316 11 -2.9840086097901093 2316 12 -2.699866171816101 2316 13 -2.9664763534175513 2316 14 -3.3593629206903657 2316 15 -3.219172053171839 2316 16 -3.1971207273514253 2316 17 -3.391781888661523 2316 18 -3.2511276195081003 2316 19 -2.935365659305951 2316 20 -2.9703674860959026 2316 21 -3.2532437766675706 2316 22 -3.3474280793819986 2316 23 -3.0857770308523023 2316 24 -2.9575175787848416 2316 25 -3.1949202659835763 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7371.0 3 0.0 4 58.0 5 275.0 6 281.0 7 244.0 8 246.0 9 235.0 10 252.0 11 201.0 12 207.0 13 225.0 14 186.0 15 235.0 16 239.0 17 268.0 18 325.0 19 403.0 20 544.0 21 709.0 22 809.0 23 989.0 24 1132.0 25 1346.0 26 1402.0 27 1735.0 28 2014.0 29 2440.0 30 3129.0 31 4192.0 32 5411.0 33 7387.0 34 10664.0 35 15163.0 36 26639.0 37 26653.0 38 31372.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.83888992844284 22.17949297010601 18.39257715461895 10.589039946832193 2 17.302706980297984 29.54415215848933 37.53479233749932 15.618348523713365 3 17.171137107129262 37.55879753001981 34.600370367980595 10.669694994870339 4 9.014008168743265 28.9758099379924 34.43002690652403 27.58015498674031 5 6.436272833444099 44.22025925758641 34.529393925707026 14.814073983262466 6 17.86283479910441 41.410882624321694 22.307895806582742 18.41838676999116 7 14.672121098715326 48.2717236306386 22.76795220059233 14.288203070053749 8 21.067098547563894 37.55105464540815 23.047986527380775 18.333860279647183 9 24.93337893032049 22.855704892857833 21.6291029222937 30.581813254527972 10 19.42754273104445 33.502816474277495 25.541195372335963 21.528445422342095 11 22.0156019124925 34.239035752769695 21.89365147985882 21.851710854878988 12 23.95390402694524 39.0176860389338 25.865751285641466 11.162658648479491 13 27.857608351991537 31.898103638510527 29.131312870609943 11.112975138887993 14 21.51812157619321 37.706557578025695 29.167446332131036 11.60787451365006 15 22.497596479568465 36.33090507868707 30.87668811015544 10.294810331589034 16 12.54347307089256 41.547673585794385 31.463856859873147 14.444996483439906 17 11.787896580871204 42.63296791219569 30.335976668107705 15.243158838825405 18 10.251579225840587 36.95291680915725 39.688090798226874 13.107413166775281 19 12.139552590317523 39.89521296158884 37.11938882830799 10.845845619785651 20 12.442170330556648 38.08337796245991 39.26868454842852 10.20576715855492 21 13.76684883953517 39.419670798355924 36.86516411689175 9.948316245217155 22 12.507984849755777 38.6679657506404 36.821287770759 12.002761628844825 23 13.991482771970576 37.2938443670151 37.38611433733385 11.328558523680474 24 11.222020763835568 38.901542769758876 36.37865286712565 13.497783599279911 25 11.795639465482866 40.66498474006492 37.56008801078842 9.979287783663803 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 24227.0 1 24227.0 2 14327.0 3 4427.0 4 4427.0 5 4427.0 6 4397.0 7 4367.0 8 4367.0 9 4367.0 10 3541.0 11 2715.0 12 2715.0 13 2715.0 14 1986.5 15 1258.0 16 1258.0 17 1258.0 18 2609.0 19 3960.0 20 3960.0 21 3960.0 22 6289.0 23 8618.0 24 8618.0 25 8618.0 26 11954.5 27 15291.0 28 15291.0 29 15291.0 30 14682.5 31 14074.0 32 14074.0 33 14074.0 34 11731.0 35 9388.0 36 9388.0 37 9388.0 38 8051.5 39 6715.0 40 6715.0 41 6715.0 42 11775.0 43 16835.0 44 16835.0 45 16835.0 46 14835.5 47 12836.0 48 12836.0 49 12836.0 50 11709.0 51 10582.0 52 10582.0 53 10582.0 54 9088.0 55 7594.0 56 7594.0 57 7594.0 58 6269.5 59 4945.0 60 4945.0 61 4945.0 62 4367.0 63 3789.0 64 3789.0 65 3789.0 66 2798.0 67 1807.0 68 1807.0 69 1807.0 70 1392.0 71 977.0 72 977.0 73 977.0 74 688.5 75 400.0 76 400.0 77 400.0 78 266.0 79 132.0 80 132.0 81 132.0 82 84.0 83 36.0 84 36.0 85 36.0 86 21.0 87 6.0 88 6.0 89 6.0 90 3.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.418083507010537 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 6.452403843051729E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 154981.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.642375516998857 #Duplication Level Percentage of deduplicated Percentage of total 1 79.139064177424 19.501745375239548 2 10.217066848210312 5.035455959117569 3 3.7260087455159594 2.754531200598783 4 1.8276557304076877 1.8015111529800425 5 1.063077688460632 1.309837980139501 6 0.6493676520646225 0.9601176918460973 7 0.476552067240973 0.8220362496047903 8 0.30111806446545 0.5936211535607591 9 0.25922337723547434 0.574909182415909 >10 1.7595768636589773 8.501687303604959 >50 0.28278913880233564 5.05674889179964 >100 0.23303919771673953 12.067285667275343 >500 0.023565761566861302 4.16502668068989 >1k 0.034039433374355214 19.270749317658293 >5k 0.005236835903746956 9.336628360895851 >10k+ 0.002618417951873478 8.248107832573025 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAA 12783 8.248107832573025 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 9084 5.86136365102819 No Hit GTATCAACGCAGAGTACTTTTTTTT 5386 3.4752647098676612 No Hit TATCAACGCAGAGTACTTTTTTTTT 4088 2.6377426910395467 No Hit GGTATCAACGCAGAGTACTTTTTTT 3901 2.5170827391744797 No Hit GGTATCAACGCAGAAAAAAAAAAAA 3400 2.193817306637588 No Hit GTACATAAGCAGTGGTATCAACGCA 2767 1.7853801433724135 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2640 1.7034346145656565 No Hit GGTATCAACGCAGAGAAAAAAAAAA 2315 1.4937314896664753 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2018 1.302095095527839 No Hit GTATCAACGCAGAGAAAAAAAAAAA 1933 1.2472496628618992 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1813 1.1698208167452784 No Hit GTATCAACGCAGAAAAAAAAAAAAA 1551 1.0007678360573231 No Hit GCTTATGTACTCTGCGTTGATACCA 1176 0.7588026919428833 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 1137 0.7336383169549816 No Hit TATCAACGCAGAGAAAAAAAAAAAA 1127 0.7271859131119299 No Hit ANAAAAAAAAAAAAAAAAAAAAAAA 991 0.6394332208464263 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 810 0.52264471128719 No Hit CTTATGTACTCTGCGTTGATACCAC 809 0.5219994709028849 No Hit TATCAACGCAGAAAAAAAAAAAAAA 748 0.4826398074602693 No Hit GAGTACATAAGCAGTGGTATCAACG 725 0.46779927862125037 No Hit TNTTTTTTTTTTTTTTTTTTTTTTT 665 0.42908485556294 No Hit ATCAACGCAGAGTACTTTTTTTTTT 622 0.40133951903781756 No Hit GTGGTATCAACGCAGAGTACTTTTT 560 0.3613346152108968 No Hit TATGTACTCTGCGTTGATACCACTG 525 0.3387512017602158 No Hit GTGGTATCAACGCAGAAAAAAAAAA 490 0.31616778830953474 No Hit GAGAAAAAAAAAAAAAAAAAAAAAA 442 0.28519624986288644 No Hit CATAAGCAGTGGTATCAACGCAGAG 432 0.2787438460198347 No Hit GCGTTGATACCACTGCTTATGTACT 425 0.2742271633296985 No Hit ACGCAGAGAAAAAAAAAAAAAAAAA 422 0.27229144217678297 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 410 0.2645485575651209 No Hit AGAAAAAAAAAAAAAAAAAAAAAAA 381 0.24583658642027087 No Hit GNATCAACGCAGAGTACTTTTTTTT 378 0.24390086526735535 No Hit ACAAAAAAAAAAAAAAAAAAAAAAA 360 0.23228653834986226 No Hit GTGGTATCAACGCAGAGTACATGGG 356 0.22970557681264153 No Hit ACGCAGAGTACATAAGCAGTGGTAT 340 0.2193817306637588 No Hit GTGGTATCAACGCAGAGAAAAAAAA 339 0.2187364902794536 No Hit TATCAACGCAGAGTACATAAGCAGT 336 0.2168007691265381 No Hit GTACTGGTTCACTATCGGTCAGTCA 334 0.21551028835792774 No Hit CTGCTTATGTACTCTGCGTTGATAC 331 0.21357456720501222 No Hit GTATCAACGCAGAGTACATAAGCAG 309 0.19937927875029843 No Hit GGTATCAACGCAGAGTACATGGGGG 299 0.1929268749072467 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 293 0.18905543260141566 No Hit ACGCAGAAAAAAAAAAAAAAAAAAA 286 0.18453874991127944 No Hit GGTATCAACGCAGAGTACATAAGCA 280 0.1806673076054484 No Hit TATTTTTTTTTTTTTTTTTTTTTTT 273 0.17615062491531222 No Hit TNTCAACGCAGAGTACTTTTTTTTT 263 0.16969822107226046 No Hit TCTTTTTTTTTTTTTTTTTTTTTTT 260 0.16776249991934494 No Hit GNTATCAACGCAGAGTACTTTTTTT 258 0.1664720191507346 No Hit AAGCAGTGGTATCAACGCAGAGTAC 255 0.16453629799781908 No Hit GTATCAACGCAGAGTAAAAAAAAAA 251 0.1619553364605984 No Hit GNTATCAACGCAGAAAAAAAAAAAA 251 0.1619553364605984 No Hit CAACGCAGAGTACTTTTTTTTTTTT 249 0.16066485569198805 No Hit TGTTTTTTTTTTTTTTTTTTTTTTT 242 0.15614817300185185 No Hit GGATACCACGTGTCCCGCCCTACTC 241 0.15550293261754666 No Hit ATAAAAAAAAAAAAAAAAAAAAAAA 236 0.1522767306960208 No Hit GTGGTATCAACGCAGAGTACATAAG 228 0.1471148076215794 No Hit AACGCAGAGTACTTTTTTTTTTTTT 227 0.14646956723727425 No Hit GTACATGGGGAAAAAAAAAAAAAAA 221 0.14259812493144322 No Hit ACTCTGCGTTGATACCACTGCTTAT 215 0.13872668262561216 No Hit GGTATCAACGCAGAGTACATGGGGA 210 0.13550048070408632 No Hit ACATAAGCAGTGGTATCAACGCAGA 207 0.13356475955117078 No Hit TCAACGCAGAGTACTTTTTTTTTTT 206 0.13291951916686562 No Hit ATACAGGGTGACAGCCCCGTACACA 204 0.13162903839825527 No Hit ATCATTAACTGAATCCATAGGTTAA 203 0.1309837980139501 No Hit CCCATGTACTCTGCGTTGATACCAC 202 0.1303385576296449 No Hit GTACATGGGGGGTCTTGAGGGGTTT 202 0.1303385576296449 No Hit GAATCAACGCAGAGTACTTTTTTTT 200 0.1290480768610346 No Hit GAGTAAAAAAAAAAAAAAAAAAAAA 199 0.1284028364767294 No Hit GGTATCAACGCAGAGTAAAAAAAAA 187 0.12065995186506732 No Hit GNACTTTTTTTTTTTTTTTTTTTTT 185 0.11936947109645699 No Hit GNTATCAACGCAGAGAAAAAAAAAA 182 0.11743374994354146 No Hit GGATCAACGCAGAGTACTTTTTTTT 179 0.11549802879062596 No Hit TATCAACGCAGAGTAAAAAAAAAAA 171 0.11033610571618456 No Hit GTACATGGGAAAAAAAAAAAAAAAA 170 0.1096908653318794 No Hit GTATCAACGCAGAGTACATGGGGAA 168 0.10840038456326904 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTCAAC 40 8.283774E-6 19.96828 1 CTCAACG 45 2.2864499E-5 17.749584 2 TTCAACG 60 1.5865842E-5 14.976211 2 TTTCAAC 55 1.2725127E-4 14.522387 1 TTTCCGA 40 0.005452702 14.169168 15 TTCCGAA 40 0.005452702 14.169168 16 TCCGAAT 40 0.005452702 14.169168 17 GCATCAA 50 0.0010227212 13.977797 1 CATCAAC 55 0.0020976472 12.707088 2 GGATCAA 80 1.6970247E-5 12.480176 1 TGTTTTT 85 3.1590014E-5 11.746047 1 TACTGGT 95 7.697177E-6 11.560583 2 TGGTTCA 90 7.819786E-6 11.545248 5 CTGGTTC 90 7.819786E-6 11.545248 4 ATCTAAG 70 9.7515364E-4 11.410445 2 GATCAAC 90 5.660553E-5 11.09349 2 GGTTCAC 95 1.4254252E-5 10.937602 6 GTACTGG 110 3.2690805E-6 10.89179 1 CGGTCAG 105 3.6402398E-6 10.795556 16 CATCTAA 75 0.0017352354 10.64975 1 >>END_MODULE