FastQCFastQC Report
Thu 26 May 2016
SRR1512154_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512154_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154981
Sequences flagged as poor quality0
Sequence length25
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT135068.714616630425665No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA117327.569960188668288No Hit
GTATCAACGCAGAGTACTTTTTTTT74344.796717016924655No Hit
TATCAACGCAGAGTACTTTTTTTTT59493.8385350462314736No Hit
GGTATCAACGCAGAGTACTTTTTTT53743.4675218252559996No Hit
GTACTTTTTTTTTTTTTTTTTTTTT35472.2886676431304482No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31792.0512191817061445No Hit
GGTATCAACGCAGAAAAAAAAAAAA29901.929268749072467No Hit
GTACATAAGCAGTGGTATCAACGCA27661.784734902988108No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23761.5330911531090907No Hit
GGTATCAACGCAGAGAAAAAAAAAA21071.3595214897309993No Hit
GTATCAACGCAGAGAAAAAAAAAAA15871.0239964898923095No Hit
GTATCAACGCAGAAAAAAAAAAAAA13840.8930126918783593No Hit
GCTTATGTACTCTGCGTTGATACCA12230.7891289900052265No Hit
GCAGAGTACTTTTTTTTTTTTTTTT11380.7342835573392867No Hit
TATCAACGCAGAGAAAAAAAAAAAA10480.6762119227518212No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA10260.6620166342971073No Hit
GTGGTATCAACGCAGAGTACTTTTT8900.5742639420316039No Hit
ATCAACGCAGAGTACTTTTTTTTTT8900.5742639420316039No Hit
CTTATGTACTCTGCGTTGATACCAC8630.5568424516553642No Hit
GAGTACATAAGCAGTGGTATCAACG7910.5103851439853918No Hit
TATCAACGCAGAAAAAAAAAAAAAA7440.48005884592304865No Hit
CATAAGCAGTGGTATCAACGCAGAG5790.3735941825126951No Hit
TATGTACTCTGCGTTGATACCACTG5530.3568179325207606No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT5450.3516560094463192No Hit
GTGGTATCAACGCAGAAAAAAAAAA4640.29939153831760024No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT4470.2884224517844123No Hit
ACGCAGAGAAAAAAAAAAAAAAAAA4450.28713197101580196No Hit
GAGAAAAAAAAAAAAAAAAAAAAAA4290.27680812486691914No Hit
GCGTTGATACCACTGCTTATGTACT4280.276162884482614No Hit
GTGGTATCAACGCAGAGTACATGGG3840.24777230757318638No Hit
TATCAACGCAGAGTACATAAGCAGT3730.2406746633458295No Hit
ACGCAGAGTACATAAGCAGTGGTAT3680.23744846142430362No Hit
CAACGCAGAGTACTTTTTTTTTTTT3650.2355127402713881No Hit
GGTATCAACGCAGAGTACATAAGCA3510.2264793748911157No Hit
AACGCAGAGTACTTTTTTTTTTTTT3460.22325317296958982No Hit
GTATCAACGCAGAGTACATAAGCAG3290.21228408643640187No Hit
GGTATCAACGCAGAGTACATGGGGG3270.21099360566779152No Hit
GTACTGGTTCACTATCGGTCAGTCA3180.20518644220904497No Hit
CTGCTTATGTACTCTGCGTTGATAC3100.20002451913460362No Hit
ACGCAGAAAAAAAAAAAAAAAAAAA3090.19937927875029843No Hit
TCAACGCAGAGTACTTTTTTTTTTT2940.18970067298572083No Hit
GTGGTATCAACGCAGAGAAAAAAAA2700.17421490376239668No Hit
ACATAAGCAGTGGTATCAACGCAGA2690.1735696633780915No Hit
AAGCAGTGGTATCAACGCAGAGTAC2640.17034346145656565No Hit
ATCATTAACTGAATCCATAGGTTAA2460.15872913453907253No Hit
ACTCTGCGTTGATACCACTGCTTAT2450.15808389415476737No Hit
GGATACCACGTGTCCCGCCCTACTC2330.15034100954310528No Hit
GGTATCAACGCAGAGTACATGGGGA2260.1458243268529691No Hit
GTATCAACGCAGAGTAAAAAAAAAA2240.14453384608435874No Hit
GTGGTATCAACGCAGAGTACATAAG2200.14195288454713803No Hit
CCCATGTACTCTGCGTTGATACCAC2160.13937192300991735No Hit
TATCAACGCAGAGTAAAAAAAAAAA1960.12646711532381388No Hit
GTACATGGGGAAAAAAAAAAAAAAA1920.12388615378659319No Hit
GGTTAATGAGGCGAACCGGGGGAAC1910.12324091340228803No Hit
ATACAGGGTGACAGCCCCGTACACA1890.12195043263367768No Hit
GTACATGGGGGGTCTTGAGGGGTTT1830.11807899032784663No Hit
GAGTAAAAAAAAAAAAAAAAAAAAA1600.10323846148882768No Hit
ATCAACGCAGAGAAAAAAAAAAAAA1570.10130274033591213No Hit
GGTATCAACGCAGAGTAAAAAAAAA1560.10065749995160696No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCCC508.67241E-515.19199810
TGTCCCG508.67241E-515.19199811
AAGCGAC400.005269152614.24249714
CCCGCCC604.073265E-412.65999814
GTCCCGC604.073265E-412.65999812
TCCCGCC604.073265E-412.65999813
ATCATTA657.5851433E-411.7582421
CCACGTG657.987349E-411.6861526
CGTGTCC657.987349E-411.6861529
ACGTGTC657.987349E-411.6861528
GCCCTAC600.005858224411.07749817
CCTACTC600.005858224411.07749819
CGCCCTA600.005858224411.07749816
CCGCCCT600.005858224411.07749815
TAACTGA600.005858224411.0774986
CATAGGT600.005858224411.07749816
CCCTACT600.005858224411.07749818
GGTATCA23050.010.3617911
CACGTGT750.002636134410.1279987
CCATAGG750.002636134410.12799815
Produced by FastQC (version 0.11.5)