FastQCFastQC Report
Thu 26 May 2016
SRR1512153_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512153_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244960
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54060.24080607226854822No Hit
TATCAACGCAGAGTACTTTTTTTTT34090.15185125792887177No Hit
GGTATCAACGCAGAGTACTTTTTTT33110.1474859240253724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14150.012.76087951
GTATTAG1800.012.059691
TACACCG907.837414E-611.56036955
GCGTGCG1251.954868E-811.3504349
GGCGAGG4200.011.26035219
TTAGGAC6050.011.100043
CTAGTCG600.006070726611.03514514
TACGACA600.00607173311.0348994
CGTGCGC1303.484638E-810.91387810
ACCGTCA803.927021E-410.6410328
TATACTG3400.010.29305655
TAGGACC3800.010.2052084
AGGACGT8250.010.2036995
CCATGCG750.002745809710.0892759
GTATAGA2500.09.8670191
TGGCGAG7700.09.82721618
GCCGGTT1752.7757778E-99.72894411
TAGGACG8800.09.673454
GGACGTG8750.09.6206296
GCTCTAT2501.8189894E-129.4723391
Produced by FastQC (version 0.11.5)