##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512153_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2244960 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.30445620411945 31.0 30.0 34.0 24.0 34.0 2 26.798044508588127 31.0 26.0 34.0 10.0 34.0 3 28.463176626755043 31.0 28.0 34.0 16.0 34.0 4 32.78318366474235 35.0 33.0 37.0 26.0 37.0 5 33.16923820468961 35.0 35.0 37.0 27.0 37.0 6 33.42299239184663 37.0 35.0 37.0 28.0 37.0 7 33.47338482645571 37.0 35.0 37.0 28.0 37.0 8 33.51615930796094 37.0 35.0 37.0 28.0 37.0 9 35.09485781483857 39.0 35.0 39.0 29.0 39.0 10 34.95593551778205 39.0 35.0 39.0 28.0 39.0 11 34.9779826812059 39.0 35.0 39.0 28.0 39.0 12 34.877068633739576 39.0 35.0 39.0 27.0 39.0 13 34.86705553773787 39.0 35.0 39.0 27.0 39.0 14 35.965405174257 40.0 36.0 41.0 27.0 41.0 15 35.896579003634805 40.0 36.0 41.0 27.0 41.0 16 35.85856629962227 40.0 36.0 41.0 27.0 41.0 17 35.79297537595325 40.0 36.0 41.0 27.0 41.0 18 35.75062228280237 40.0 36.0 41.0 27.0 41.0 19 35.773362108901715 40.0 36.0 41.0 27.0 41.0 20 35.697797288147676 40.0 36.0 41.0 27.0 41.0 21 35.6344741108973 40.0 36.0 41.0 27.0 41.0 22 35.53060544508588 40.0 36.0 41.0 27.0 41.0 23 35.41971482788112 39.0 36.0 41.0 27.0 41.0 24 35.30666158862519 39.0 36.0 41.0 26.0 41.0 25 35.17121774998218 39.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7370093939392355 1101 2 3.1634159557044583 1101 3 1.6121163664736038 1101 4 0.8026204597398916 1101 5 0.5778921610102827 1101 6 0.18061538511823017 1101 7 0.04852221333563023 1101 8 0.11604175636925618 1101 9 -0.006228282225478665 1101 10 -0.2941041266573876 1101 11 0.03422794550320418 1101 12 -0.2352663981658054 1101 13 -0.07566642910800425 1101 14 -0.12537947435694008 1101 15 0.031576420273623285 1101 16 0.07411602950884344 1101 17 -0.1376324955786501 1101 18 -0.250705651652666 1101 19 -0.05882603595999569 1101 20 -0.2775583766172076 1101 21 -0.19238888591855385 1101 22 -0.1392989406595717 1101 23 -0.20032482484494096 1101 24 -0.36226848640731646 1101 25 -0.3404383724848259 1102 1 1.10480389188562 1102 2 3.5973400206556825 1102 3 2.2110667403370954 1102 4 1.3098539479233082 1102 5 1.012273170227175 1102 6 0.7974078431000322 1102 7 0.5801658475149836 1102 8 0.5994488092640182 1102 9 0.6628958981807145 1102 10 0.5676326906078089 1102 11 0.7873208516531278 1102 12 0.7227206677566471 1102 13 0.7460108274321726 1102 14 0.7127419249885563 1102 15 0.6738038821754344 1102 16 0.6220356946894157 1102 17 0.515217799104569 1102 18 0.5700122754624815 1102 19 0.4641836333941214 1102 20 0.6296762817179768 1102 21 0.579971544229231 1102 22 0.7841464152030824 1102 23 0.6343672497171298 1102 24 0.45339906567419064 1102 25 0.5236736578455137 1103 1 0.9424353851752514 1103 2 2.810463667152046 1103 3 1.7691254845115019 1103 4 1.1177782589652665 1103 5 0.7804378240030658 1103 6 0.7987329831306198 1103 7 0.6373491300164744 1103 8 0.3401046246964512 1103 9 0.5092048667239268 1103 10 0.4080185108756993 1103 11 0.49365811794738335 1103 12 0.5222576307531952 1103 13 0.5507587911794403 1103 14 0.733698253505878 1103 15 0.4644996794702152 1103 16 0.42321012661770396 1103 17 0.4507277136656782 1103 18 0.4447719609510443 1103 19 0.7004835895551835 1103 20 0.741385040662152 1103 21 0.7541662154466167 1103 22 0.6496715134643907 1103 23 0.7349902141699616 1103 24 0.6955994039677833 1103 25 0.6109769235640243 1104 1 1.3219462370150232 1104 2 3.885748305572303 1104 3 2.461125217662982 1104 4 1.6645019521487683 1104 5 1.2751230909998341 1104 6 0.9833506485025794 1104 7 0.9393467876794759 1104 8 0.8297872232080294 1104 9 0.8508165576540421 1104 10 0.9688607144514734 1104 11 0.7987371347588379 1104 12 0.8491326122220499 1104 13 0.8095992602398354 1104 14 0.8609027187710936 1104 15 0.6360888365253885 1104 16 0.6381480843370397 1104 17 0.8039043229104976 1104 18 0.8991038019093267 1104 19 0.8874613818284374 1104 20 0.7217516608774162 1104 21 0.8783860541515125 1104 22 0.9263604422158309 1104 23 1.038758708254818 1104 24 1.162319687420208 1104 25 1.0667808788520787 1105 1 1.2801506032619336 1105 2 3.6854717373717634 1105 3 2.083409553310858 1105 4 1.5771652450756832 1105 5 1.1285566557874063 1105 6 0.8900559562582586 1105 7 0.5729704195495486 1105 8 0.4995795028361343 1105 9 0.608770835634509 1105 10 0.6317976333640303 1105 11 0.42695591785875564 1105 12 0.4403250919207693 1105 13 0.5338945997714362 1105 14 0.5974597388958429 1105 15 0.6166922848012533 1105 16 0.5162271122292381 1105 17 0.7386504097646878 1105 18 0.5018753683445354 1105 19 0.7387235920202713 1105 20 0.7493408099888654 1105 21 0.6208172063228687 1105 22 0.8097589946461028 1105 23 0.6990564604026375 1105 24 0.7248770606107442 1105 25 0.7067979436984899 1106 1 0.39620773775449436 1106 2 2.9516450129748115 1106 3 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0.8114863107934305 1109 2 3.2146115849647856 1109 3 1.6433114072677206 1109 4 1.2225469685039414 1109 5 0.741861566726449 1109 6 0.4311351348636592 1109 7 0.3748811047248779 1109 8 0.18860280601420243 1109 9 0.19014084400647846 1109 10 0.17941340404900075 1109 11 0.34276785654796527 1109 12 0.2371589318035916 1109 13 0.2887332645916132 1109 14 0.22599090702130553 1109 15 0.18856859069343557 1109 16 0.09914297604705524 1109 17 0.32732806601178766 1109 18 0.2526601516074365 1109 19 0.28797293131905377 1109 20 0.22974083908396636 1109 21 0.31059944233832226 1109 22 0.31567464167690673 1109 23 0.2935795218346584 1109 24 0.20555894848336465 1109 25 0.09202116301421626 1110 1 0.25276954180558064 1110 2 2.071628310200861 1110 3 1.0330038337240808 1110 4 0.48724553002426063 1110 5 0.3241424152303338 1110 6 0.24978492297608312 1110 7 0.3164036032314286 1110 8 0.17401888254615017 1110 9 0.21213627531199108 1110 10 0.2642176920029655 1110 11 0.09059376516847095 1110 12 0.055094773758732174 1110 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2315 18 -1.360277089655476 2315 19 -1.1677019095417478 2315 20 -1.3632495554456412 2315 21 -1.2761819991234375 2315 22 -1.5417407254543036 2315 23 -1.2062878161020478 2315 24 -1.2052029665966941 2315 25 -1.361173265768734 2316 1 -1.3874991994348314 2316 2 -1.6022281009612733 2316 3 -1.0034538467946028 2316 4 -1.4301821998329665 2316 5 -1.6148716399878147 2316 6 -1.6556663879706939 2316 7 -1.7481232082224025 2316 8 -1.8862613102501982 2316 9 -2.064944375546588 2316 10 -1.9730381790559335 2316 11 -2.163352321361998 2316 12 -2.227636165952532 2316 13 -2.1765542143810848 2316 14 -2.338276193470577 2316 15 -2.5760072058608827 2316 16 -2.5539886465335186 2316 17 -2.5050654388130553 2316 18 -2.2510045055668186 2316 19 -2.526571754404806 2316 20 -2.397516824574261 2316 21 -2.436814314679353 2316 22 -2.295401310608945 2316 23 -2.3153979570458034 2316 24 -2.2169452853565588 2316 25 -2.5878031618949535 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 78938.0 3 6.0 4 683.0 5 3329.0 6 3913.0 7 3527.0 8 3353.0 9 3195.0 10 3025.0 11 3083.0 12 3053.0 13 2908.0 14 3111.0 15 3028.0 16 3258.0 17 3567.0 18 4170.0 19 5059.0 20 6669.0 21 8408.0 22 10571.0 23 12663.0 24 14451.0 25 16620.0 26 19311.0 27 22249.0 28 26238.0 29 32130.0 30 41064.0 31 52710.0 32 69959.0 33 93722.0 34 128064.0 35 177407.0 36 260464.0 37 367551.0 38 753503.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.52262846554059 19.01298909557409 13.208074976837004 25.256307462048323 2 16.25528917240309 21.717181573931317 38.65633413536378 23.371195118301817 3 18.132661606442877 24.94988774855677 30.177375097997295 26.740075547003066 4 12.632029078469104 17.22966110754757 37.90811417575369 32.23019563822964 5 13.59658969424845 37.42962012686195 35.41230133276317 13.561488846126435 6 31.218819043546432 36.90738365048821 18.450662818045757 13.423134487919608 7 27.339150808923097 31.925379516784265 22.72949183949825 18.005977834794386 8 25.294638553841192 35.68335921359636 21.156959118093287 17.865043114469163 9 26.31703905637517 15.513104910555201 20.477603164421637 37.692252868648 10 15.358358278098496 28.745768298767015 33.89846589694249 21.997407526192003 11 33.61547644501461 22.989407383650487 24.386046967429266 19.00906920390564 12 22.732743567814126 24.929486494191433 31.413210034922674 20.924559903071767 13 28.332486993086736 21.928007625971063 26.11338286650987 23.62612251443233 14 21.628391163819984 21.505805023893608 27.694712403650502 29.171091408635906 15 23.341039483999715 29.358518637303117 23.972943838643005 23.327498040054166 16 22.719736654550637 27.909049604447294 26.7736173473024 22.597596393699664 17 21.983955170693463 27.93216805644644 27.560758320860952 22.523118451999142 18 21.826714061720477 27.49492195852042 28.73721580785404 21.941148171905066 19 23.280099921780202 27.010685287373114 27.623213996176315 22.086000794670362 20 23.286593017149105 27.06105283976486 27.907884204463606 21.744469938622434 21 23.372141762945336 26.60079760922137 27.358094290363432 22.668966337469858 22 23.293457131301036 26.876836345429826 27.53600528831848 22.293701234950653 23 23.07393174633366 26.717221638442567 27.374653054479186 22.834193560744588 24 22.943780214943985 26.685945432362402 27.700352389823056 22.66992196287056 25 23.036401539448363 26.693081391205187 27.65724110897299 22.613275960373457 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 948.0 1 948.0 2 907.0 3 866.0 4 866.0 5 866.0 6 1876.5 7 2887.0 8 2887.0 9 2887.0 10 4048.5 11 5210.0 12 5210.0 13 5210.0 14 8305.0 15 11400.0 16 11400.0 17 11400.0 18 19940.5 19 28481.0 20 28481.0 21 28481.0 22 42960.5 23 57440.0 24 57440.0 25 57440.0 26 79629.5 27 101819.0 28 101819.0 29 101819.0 30 129691.5 31 157564.0 32 157564.0 33 157564.0 34 178633.0 35 199702.0 36 199702.0 37 199702.0 38 219477.5 39 239253.0 40 239253.0 41 239253.0 42 255938.5 43 272624.0 44 272624.0 45 272624.0 46 280029.0 47 287434.0 48 287434.0 49 287434.0 50 282977.0 51 278520.0 52 278520.0 53 278520.0 54 253207.5 55 227895.0 56 227895.0 57 227895.0 58 196772.0 59 165649.0 60 165649.0 61 165649.0 62 135952.0 63 106255.0 64 106255.0 65 106255.0 66 82588.5 67 58922.0 68 58922.0 69 58922.0 70 42805.5 71 26689.0 72 26689.0 73 26689.0 74 18449.5 75 10210.0 76 10210.0 77 10210.0 78 6920.5 79 3631.0 80 3631.0 81 3631.0 82 2366.0 83 1101.0 84 1101.0 85 1101.0 86 725.5 87 350.0 88 350.0 89 350.0 90 214.5 91 79.0 92 79.0 93 79.0 94 53.0 95 27.0 96 27.0 97 27.0 98 15.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.139138336540517 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.6726534103057513E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 3.5635378804076687E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 1.7817689402038344E-4 20 1.3363267051528756E-4 21 4.454422350509586E-5 22 5.345306820611503E-4 23 4.899864585560544E-4 24 1.3363267051528756E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2244960.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.05385166812434 #Duplication Level Percentage of deduplicated Percentage of total 1 80.64788512829062 56.49694982125203 2 12.55273761113741 17.58735237279011 3 3.5122278275001375 7.381352617570598 4 1.3450209160396374 3.7689558297106194 5 0.6405363821704937 2.2436020352304387 6 0.36950097487260103 1.5530979890971521 7 0.22961299480315261 1.1259692275309703 8 0.13549705267287304 0.7593672343530784 9 0.11606845096152904 0.7317937842307128 >10 0.4241651329554023 4.899936651720756 >50 0.016537079569857952 0.7919642572250309 >100 0.008350591685649418 1.1247950458579994 >500 0.0011543942114810492 0.5399404366591994 >1k 6.413301174880088E-4 0.7520438483293671 >5k 6.413301174880088E-5 0.24287884844187577 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5406 0.24080607226854822 No Hit TATCAACGCAGAGTACTTTTTTTTT 3409 0.15185125792887177 No Hit GGTATCAACGCAGAGTACTTTTTTT 3311 0.1474859240253724 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.454422350509586E-5 0.0 0.0 0.0 0.0 7 4.454422350509586E-5 0.0 0.0 0.0 0.0 8 4.454422350509586E-5 0.0 0.0 0.0 0.0 9 4.454422350509586E-5 0.0 0.0 0.0 0.0 10 4.454422350509586E-5 0.0 0.0 4.454422350509586E-5 0.0 11 4.454422350509586E-5 0.0 0.0 4.454422350509586E-5 0.0 12 4.454422350509586E-5 0.0 0.0 4.454422350509586E-5 0.0 13 4.454422350509586E-5 0.0 0.0 4.454422350509586E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1415 0.0 12.7608795 1 GTATTAG 180 0.0 12.05969 1 TACACCG 90 7.837414E-6 11.5603695 5 GCGTGCG 125 1.954868E-8 11.350434 9 GGCGAGG 420 0.0 11.260352 19 TTAGGAC 605 0.0 11.10004 3 CTAGTCG 60 0.0060707266 11.035145 14 TACGACA 60 0.006071733 11.034899 4 CGTGCGC 130 3.484638E-8 10.913878 10 ACCGTCA 80 3.927021E-4 10.641032 8 TATACTG 340 0.0 10.2930565 5 TAGGACC 380 0.0 10.205208 4 AGGACGT 825 0.0 10.203699 5 CCATGCG 75 0.0027458097 10.089275 9 GTATAGA 250 0.0 9.867019 1 TGGCGAG 770 0.0 9.827216 18 GCCGGTT 175 2.7757778E-9 9.728944 11 TAGGACG 880 0.0 9.67345 4 GGACGTG 875 0.0 9.620629 6 GCTCTAT 250 1.8189894E-12 9.472339 1 >>END_MODULE