FastQCFastQC Report
Thu 26 May 2016
SRR1512153_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512153_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244960
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63910.28468213242106766No Hit
TATCAACGCAGAGTACTTTTTTTTT46670.20788789109828237No Hit
GGTATCAACGCAGAGTACTTTTTTT45520.20276530539519635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16000.012.9297691
GTATTAG1600.012.5126791
TCCAACG2001.8189894E-1210.44687718
GCGTGCA1002.4081977E-510.4466439
TGGCGAG8150.010.13807318
TATTCCG750.00264367110.1354965
TTAGGAC6800.09.9212353
CGGTTTC1554.0852683E-89.80330213
TAGGACC3650.09.6321934
GGCGAGG5250.09.58782319
GGACGTG8550.09.5526996
GTCCTAC6700.09.533471
AGGACGT8900.09.50202755
GCACCGT800.00452792049.4971616
AGCGTGC900.00111696949.497168
GCGAGAC800.0045287529.49694911
GTATCAA30900.09.2866481
TATGGCG8600.09.1656616
ATGGCGA9350.09.03987617
GATATAC4650.09.0209181
Produced by FastQC (version 0.11.5)