FastQCFastQC Report
Thu 26 May 2016
SRR1512152_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512152_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810749
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78400.4329700030208494No Hit
TATCAACGCAGAGTACTTTTTTTTT50080.2765706345827058No Hit
GGTATCAACGCAGAGTACTTTTTTT48150.2659120618042589No Hit
GTCCTACAGTGGACATTTCTAAATT26110.14419447422033643No Hit
GTCCTAAAGTGTGTATTTCTCATTT24840.13718080197752422No Hit
CTGTAGGACGTGGAATATGGCAAGA24170.1334806756761981No Hit
CTTTAGGACGTGAAATATGGCGAGG23410.12928351748364902No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21930.12111010416131666No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21910.1209996526299338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT707.591865E-613.5135586
CGGTTTC905.6940735E-712.61265513
GGTATCA14050.012.5604061
AGGACCG951.0951499E-611.948835
ATGCCGG1850.011.76044819
TCCAACG1105.251459E-711.17939818
AATGCCG1950.011.15734818
TAGGACC4850.010.9222994
GGCGAGG6650.010.5263519
GTGCGCC909.937365E-510.51054511
CTAGACC1002.5147805E-510.4054394
GTTAGAC1002.5147805E-510.4054393
TAGGACT3200.010.3463174
GTATTAA2500.010.253221
CCAACGT750.002743458410.09012419
GGACGTG14350.010.0198096
AATGTCC4450.09.9909228
TTAGGAC10050.09.883053
GTATTAC1201.0954087E-59.8588661
GGGTAAC1104.5534034E-59.8588651
Produced by FastQC (version 0.11.5)