FastQCFastQC Report
Thu 26 May 2016
SRR1512152_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512152_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810749
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT97720.5396661823367016No Hit
TATCAACGCAGAGTACTTTTTTTTT71830.39668667496157667No Hit
GGTATCAACGCAGAGTACTTTTTTT66570.36763792220788194No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31960.17650154714982583No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27200.15021408268070285No Hit
CTTTAGGACGTGAAATATGGCGAGG25540.14104660557592466No Hit
CTGTAGGACGTGGAATATGGCAAGA24910.13756738233736426No Hit
GTCCTACAGTGGACATTTCTAAATT22940.12668790649615158No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22840.12613564883923725No Hit
GTCCTAAAGTGTGTATTTCTCATTT21230.11724430056291622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGT508.7349996E-515.1959576
GGTATCA15700.012.8108181
TAGGACC3700.012.5829474
ACCGTGC550.003074900712.0876938
GCGCCAC951.364862E-510.9967713
TCCAACG1303.279274E-810.95862318
GGCGAGG7500.010.25727119
AATCCCG1951.4551915E-1110.22804719
AGGACCT9750.09.8424855
GATATAC5300.09.7120721
TGTAGGA15100.09.5643322
GTAGGAC14450.09.53428653
GTATCAA32150.09.5173941
TGCGCCA1201.7062484E-59.49721112
CTGTAGG14650.09.434621
TGGCGAG14500.09.36647318
TATACAC7700.09.254623
TGTCGAG1054.5043248E-49.04521218
GTGCGCC1054.505568E-49.04496211
TAGACTC950.00182130159.0013345
Produced by FastQC (version 0.11.5)