FastQCFastQC Report
Thu 26 May 2016
SRR1512151_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512151_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608371
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47990.2983764318058458No Hit
GGTATCAACGCAGAGTACTTTTTTT31660.19684513088087263No Hit
TATCAACGCAGAGTACTTTTTTTTT30950.1924307264928303No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGT655.6932375E-513.0934556
TATTCCG604.2541046E-412.6085135
GGTATCA9850.012.5477721
CTATGCG550.003176100612.03539859
AGACCGC550.003176100612.03539856
TCCAACG1751.8189894E-1211.34766118
GCACTCG752.1644376E-411.3476613
CCCGTTT1303.4940967E-810.91121312
TCTACGG650.0079124410.6482332
CCAACGA1356.082519E-810.50709319
GACGTGG4850.010.333787
CCAGTAC2200.010.3160553
TAGGACC2850.010.2858914
GGACGTG9400.010.2611826
TCCCGTT1304.0905434E-710.18379911
AATGTCC3000.010.0868098
GTATCAA20300.010.0824761
AGGACGT9800.010.0353465
GTCTTAA1406.127975E-79.8876451
GTATAGG1501.478802E-79.8876441
Produced by FastQC (version 0.11.5)