FastQCFastQC Report
Thu 26 May 2016
SRR1512151_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512151_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608371
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57940.36024026794812886No Hit
TATCAACGCAGAGTACTTTTTTTTT41820.2600146359266612No Hit
GGTATCAACGCAGAGTACTTTTTTT40370.25099930302150436No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19510.12130285860662746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12800.012.7393451
ATCGCTC550.003076395512.0867748
AATCCCG1950.011.20164519
CCGTAGC600.00589214911.0791999
GGCGAGG4700.010.91150119
AGAACCG700.001487585410.8605215
GACTGCG909.547675E-510.5519467
TAGGACC3000.010.4532514
TTAGGAC6500.010.3801513
GTATAAG1303.64651E-710.2694141
GACGTGA4950.09.7845327
GTATCAA26200.09.7542631
TGGCGAG9150.09.65273618
GTCCTAC8550.09.5916491
GTATTAT1502.5057489E-79.5358841
GTCCTAG2106.184564E-119.5358841
GTATAGC2106.184564E-119.5358841
CTAGAAC1701.6550985E-89.5029553
GCACCGT900.00111704639.4967516
AGGACGT8900.09.2894055
Produced by FastQC (version 0.11.5)