FastQCFastQC Report
Thu 26 May 2016
SRR1512150_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512150_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1825408
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52250.2862373781642241No Hit
GTCCTACAGTGGACATTTCTAAATT41740.2286612088913821No Hit
GTCCTAAAGTGTGTATTTCTCATTT41490.22729165205806046No Hit
CTGTAGGACGTGGAATATGGCAAGA38970.21348651917817824No Hit
CTTTAGGACGTGAAATATGGCGAGG37270.20417353271159105No Hit
GGTATCAACGCAGAGTACTTTTTTT34540.18921797209171867No Hit
TATCAACGCAGAGTACTTTTTTTTT33440.18319192202510343No Hit
CTGAAGGACCTGGAATATGGCGAGA19830.10863324801907301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12750.012.5412611
ACCGTCC550.00317133812.0379538
CCGTCCA752.1610285E-411.349769
TAGGACC5500.011.1780994
TAAGACG700.001547236610.8095914
GATCTAG1209.319774E-710.6929731
ACTGTCG803.9274283E-410.640698
TCCAACG1803.8198777E-1110.50903618
TAGACAG2450.010.4235345
TCAATAG1304.0799569E-710.1859613
GTCCTAT2550.010.0639751
TGTCGAG1606.6356733E-910.04926618
TGTAGGA23300.010.0401742
CACCTTT6600.010.03135314
CCACCTT6600.010.03135313
AATGTCC5700.09.9562028
GACGTGG13700.09.9416677
GGCGAGG10200.09.92176619
GGACGTG25900.09.8966186
GTATAAG1708.903953E-99.8704371
Produced by FastQC (version 0.11.5)