FastQCFastQC Report
Thu 26 May 2016
SRR1512150_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512150_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1825408
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61060.3345005609704789No Hit
TATCAACGCAGAGTACTTTTTTTTT44090.24153504312460555No Hit
GGTATCAACGCAGAGTACTTTTTTT43180.23654985625131478No Hit
CTTTAGGACGTGAAATATGGCGAGG42160.23096206437136246No Hit
CTGTAGGACGTGGAATATGGCAAGA40900.22405949793142138No Hit
GTCCTACAGTGGACATTTCTAAATT40880.2239499333847556No Hit
GTCCTAAAGTGTGTATTTCTCATTT40480.22175864245144097No Hit
CTGAAGGACCTGGAATATGGCGAGA22330.12232881635228945No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19950.10929063529906738No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTA456.779414E-414.7730916
GGTATCA15350.012.6096151
TAGGACC4850.012.14684
GGCGAGG10250.011.21169219
ACTGTTC5500.010.7056948
CCAACGA1451.4493708E-810.48000819
GATATAC5200.010.4516711
AGGACCT13400.010.2819415
AGAACCG856.581799E-410.0608695
TTAGGAC15600.09.9283013
TGTAGGA21800.09.807042
GACGTGA13850.09.8055537
TAGGACA6500.09.7942984
GGACCTA1752.586603E-99.7683296
AGGACGT24550.09.6761025
CACTGTT6000.09.655277
AATGTCC7300.09.6270838
GGACGTG24300.09.6142346
ACGTGAA15150.09.5910178
CAGGACT2401.8189894E-129.5019334
Produced by FastQC (version 0.11.5)