##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512150_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1825408 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.27143466008695 31.0 30.0 34.0 26.0 34.0 2 30.474525695077485 31.0 30.0 34.0 27.0 34.0 3 30.560292274384686 31.0 30.0 34.0 27.0 34.0 4 34.156298208400536 37.0 35.0 37.0 30.0 37.0 5 33.69732081814038 37.0 35.0 37.0 28.0 37.0 6 33.62568039583479 37.0 35.0 37.0 28.0 37.0 7 33.426141443447165 37.0 35.0 37.0 28.0 37.0 8 33.30248087003015 36.0 35.0 37.0 27.0 37.0 9 34.71353144064231 38.0 35.0 39.0 27.0 39.0 10 34.482486655038215 38.0 35.0 39.0 27.0 39.0 11 34.46366291809831 38.0 35.0 39.0 27.0 39.0 12 34.264322277540145 38.0 34.0 39.0 27.0 39.0 13 34.235246585968724 38.0 34.0 39.0 27.0 39.0 14 35.12281090035762 39.0 34.0 40.0 26.0 41.0 15 35.05609869136106 39.0 34.0 40.0 26.0 41.0 16 34.9298962204614 38.0 34.0 40.0 25.0 41.0 17 34.86656352990674 38.0 34.0 40.0 25.0 41.0 18 34.78392666187504 38.0 34.0 40.0 25.0 41.0 19 34.801747335390225 39.0 34.0 40.0 25.0 41.0 20 34.68596664416941 39.0 34.0 40.0 25.0 41.0 21 34.53682245284342 39.0 34.0 40.0 24.0 41.0 22 34.40455722775401 38.0 34.0 40.0 23.0 41.0 23 34.449369675163034 39.0 34.0 40.0 23.0 41.0 24 34.31191711661174 39.0 34.0 40.0 21.0 41.0 25 33.8593947216184 38.0 33.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44295210203388535 1101 2 0.2782695898326857 1101 3 0.2988876878865554 1101 4 0.2869590262945394 1101 5 0.18481086125951407 1101 6 0.13549899417629518 1101 7 0.20688139570219022 1101 8 0.21306027665673355 1101 9 0.3084837878015918 1101 10 0.360881150718555 1101 11 0.2604018093574183 1101 12 0.19547623976147577 1101 13 0.18730898598396806 1101 14 0.34811559428104744 1101 15 0.48118161765404466 1101 16 0.36950293233354614 1101 17 0.2625786802669481 1101 18 0.23092770329559187 1101 19 0.04016955787024301 1101 20 -0.03750053170105616 1101 21 -0.23231684714421164 1101 22 -0.25371422233551755 1101 23 -0.14094210679984087 1101 24 -0.3780289102323522 1101 25 -0.21287648180349095 1102 1 0.31085452394479773 1102 2 0.2093921128783407 1102 3 0.39090518080953984 1102 4 0.37950396834771993 1102 5 0.24714240416919608 1102 6 0.3043918953884628 1102 7 0.49400879402306686 1102 8 0.37817604932132554 1102 9 0.4963917745427793 1102 10 0.6448895221588771 1102 11 0.7052413365887915 1102 12 0.8665887219177222 1102 13 0.9723232609833659 1102 14 1.0543200775986534 1102 15 0.7867304187960471 1102 16 0.8261659092757938 1102 17 0.8395021759481551 1102 18 0.7355431856212107 1102 19 0.8165432241255957 1102 20 0.9241429683352393 1102 21 1.0719798671864424 1102 22 0.923955384013226 1102 23 1.0668988600289282 1102 24 1.1069932281141632 1102 25 0.8263337961960673 1103 1 0.18271730598823055 1103 2 0.17843092770711166 1103 3 0.20140850173845948 1103 4 0.25745276480540014 1103 5 0.309049465264863 1103 6 0.27370333257344726 1103 7 0.3057174237277138 1103 8 0.4403504334185513 1103 9 0.5026532564778705 1103 10 0.29769229195490965 1103 11 0.07999579982304539 1103 12 0.28751057410105574 1103 13 0.09288593468443196 1103 14 0.3797540358632929 1103 15 0.18310507139390353 1103 16 0.2851059205810813 1103 17 0.3875178620379387 1103 18 0.2890437037803295 1103 19 0.2651418392275815 1103 20 0.3570266416123573 1103 21 0.4328723962740071 1103 22 0.3052150994967633 1103 23 0.22709364075340943 1103 24 0.45283853406357366 1103 25 0.24067738279930495 1104 1 -0.014056744910568852 1104 2 0.01861197931461689 1104 3 -0.03514558274530799 1104 4 -0.07759802576800467 1104 5 -0.19322909912320085 1104 6 -0.06280501770171298 1104 7 -0.16682983204520951 1104 8 -0.19512988632524753 1104 9 -0.2599373246026033 1104 10 -0.3794905273437621 1104 11 -0.10432469806305278 1104 12 -0.006444015204586151 1104 13 -0.10785116576673914 1104 14 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0.41533615620026154 1107 12 0.48386439259302705 1107 13 0.31614842287050493 1107 14 0.4110615207068875 1107 15 0.4903034992770827 1107 16 0.443198749238789 1107 17 0.3681793267344631 1107 18 0.37299693215219776 1107 19 0.32935543504141407 1107 20 0.45075027638333864 1107 21 0.4159784795823711 1107 22 0.39436472721396854 1107 23 0.2403724955515969 1107 24 0.37713661386809605 1107 25 0.16650493403140842 1108 1 0.025413871996629922 1108 2 0.05490319690019163 1108 3 0.0496335658382101 1108 4 0.07400372002951627 1108 5 0.021815075621631763 1108 6 0.14343711187679276 1108 7 0.18764112505381547 1108 8 0.28961223154173865 1108 9 -0.00810391375014774 1108 10 0.05128935962332548 1108 11 -0.09187535039481531 1108 12 0.046743582182294574 1108 13 0.0524589330227343 1108 14 -0.011568337228197834 1108 15 -0.03562555664302636 1108 16 0.02307491193956679 1108 17 -0.04551182016905386 1108 18 0.03699076790483247 1108 19 0.024895745684133885 1108 20 -0.009321860776687174 1108 21 0.14344528323231032 1108 22 0.29858911465569804 1108 23 0.3005335613698463 1108 24 0.12733048939006864 1108 25 0.18558703561997447 1109 1 -0.365896405505989 1109 2 -0.3954415877828481 1109 3 -0.28827456495505643 1109 4 -0.3344094090649534 1109 5 -0.4485659820370884 1109 6 -0.47258137872717043 1109 7 -0.34121933883830735 1109 8 -0.49104632873695664 1109 9 -0.5113627391374038 1109 10 -0.5171387954795392 1109 11 -0.5070788953843177 1109 12 -0.29850288742353115 1109 13 -0.43379274783250565 1109 14 -0.3023215996426032 1109 15 -0.5725493403259279 1109 16 -0.4689383339175137 1109 17 -0.4324746044788341 1109 18 -0.360710348518019 1109 19 -0.5424963804960541 1109 20 -0.3494815001524856 1109 21 -0.4868646640809544 1109 22 -0.4899365659411359 1109 23 -0.7501935730172207 1109 24 -0.5941811079129025 1109 25 -0.5421383425425503 1110 1 -0.29006623127392217 1110 2 -0.29237737699445887 1110 3 -0.37420916568693485 1110 4 -0.274539155350368 1110 5 -0.27849359281702135 1110 6 -0.20371485016842428 1110 7 -0.24991544034293156 1110 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-0.7988791177705252 2315 8 -0.6993750621865757 2315 9 -0.8839693539278954 2315 10 -0.8118197002319789 2315 11 -0.6600865359225878 2315 12 -0.878076932934519 2315 13 -0.7935614814503467 2315 14 -0.9063988314273246 2315 15 -1.0131992749812966 2315 16 -0.9430031381306918 2315 17 -0.9020454537277658 2315 18 -0.9088535200300711 2315 19 -0.9329255733032014 2315 20 -1.0358885020691915 2315 21 -0.8111120442500379 2315 22 -0.990060105675191 2315 23 -0.9931872929931203 2315 24 -0.8684955675290809 2315 25 -0.982285969896239 2316 1 -1.371593443800652 2316 2 -1.502548063649936 2316 3 -1.391106914646489 2316 4 -1.4347408829108588 2316 5 -1.8329355080025387 2316 6 -1.912142647490473 2316 7 -1.8505033014329655 2316 8 -1.9206579923044806 2316 9 -2.119264213252798 2316 10 -2.3024407600684285 2316 11 -2.1945502619728288 2316 12 -2.102469793937132 2316 13 -2.379172325809705 2316 14 -2.479145379522407 2316 15 -2.366512743457413 2316 16 -2.66151116384178 2316 17 -2.514739406268255 2316 18 -2.489845013136552 2316 19 -2.442310431753228 2316 20 -2.419047889243494 2316 21 -2.4162257010820554 2316 22 -2.420752853973042 2316 23 -2.6016447816840476 2316 24 -2.568385435937266 2316 25 -2.5759109460944387 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40314.0 3 5.0 4 729.0 5 6362.0 6 7686.0 7 6220.0 8 5007.0 9 4511.0 10 4186.0 11 3942.0 12 3783.0 13 3669.0 14 3657.0 15 3530.0 16 3638.0 17 3674.0 18 4135.0 19 4660.0 20 5862.0 21 7201.0 22 9470.0 23 11812.0 24 14368.0 25 17070.0 26 19550.0 27 22209.0 28 25894.0 29 31371.0 30 39589.0 31 50423.0 32 64425.0 33 82656.0 34 105854.0 35 139785.0 36 201725.0 37 336991.0 38 529445.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.07117731346896 21.163809581434492 14.445199244222342 24.319813860874202 2 14.702904775261203 22.000725317298926 40.94635281537059 22.35001709206928 3 18.31552179019704 26.17354585933665 31.15424058621415 24.356691764252155 4 12.988164837669167 18.772351167519812 38.45512893555851 29.784355059252505 5 12.7045792874228 38.63065340404587 36.1781684165755 12.486598891955827 6 30.41369381530047 38.451458523245215 18.517613596521983 12.617234064932333 7 26.74311715517846 32.45438827922305 23.241653372835007 17.56084119276348 8 24.168572233112045 38.09671986913224 21.16416870371446 16.570539194041263 9 26.004652110651428 15.4724861510413 21.22714483556553 37.295716902741745 10 15.072630337984712 29.723984994039686 34.83013112684945 20.37325354112615 11 33.10262691957086 22.693611510413014 25.84863219619942 18.355129373816702 12 22.99535227193044 25.286566071804224 32.48150550452282 19.236576151742515 13 28.59831884159596 22.831662839211837 26.379417642521563 22.19060067667064 14 20.34076765303976 23.336098012060866 28.343526488324798 27.979607846574574 15 22.175809462870767 32.43970662996985 23.92949959680247 21.454984310356917 16 21.048390277680387 28.83519739148727 29.029838808638946 21.086573522193394 17 20.84985931912208 29.502116787041583 28.773348204894468 20.874675688941867 18 20.63883800224388 27.939507222494914 31.35649673935909 20.065158035902112 19 22.954973255235554 27.32255434961247 28.562499041308115 21.15997335384386 20 23.022031239043546 28.06287690204053 29.0859358565318 19.829156002384124 21 22.158553046770912 27.29378856672043 28.204105602692657 22.343552783816 22 22.584266092840615 28.931997668466447 28.312793632985063 20.170942605707875 23 21.462215570436854 28.66115410910876 28.870860651426966 21.00576966902742 24 22.48951539340216 27.48969344681271 29.471290942012114 20.549500217773016 25 21.986046779172614 28.094730700646714 29.177296844227378 20.741925675953294 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1181.0 1 1181.0 2 1020.0 3 859.0 4 859.0 5 859.0 6 1804.5 7 2750.0 8 2750.0 9 2750.0 10 3929.0 11 5108.0 12 5108.0 13 5108.0 14 7794.5 15 10481.0 16 10481.0 17 10481.0 18 18568.0 19 26655.0 20 26655.0 21 26655.0 22 42202.5 23 57750.0 24 57750.0 25 57750.0 26 81729.0 27 105708.0 28 105708.0 29 105708.0 30 134585.5 31 163463.0 32 163463.0 33 163463.0 34 180120.0 35 196777.0 36 196777.0 37 196777.0 38 207007.0 39 217237.0 40 217237.0 41 217237.0 42 224973.0 43 232709.0 44 232709.0 45 232709.0 46 231806.5 47 230904.0 48 230904.0 49 230904.0 50 217590.0 51 204276.0 52 204276.0 53 204276.0 54 177402.0 55 150528.0 56 150528.0 57 150528.0 58 126720.0 59 102912.0 60 102912.0 61 102912.0 62 82245.0 63 61578.0 64 61578.0 65 61578.0 66 47063.5 67 32549.0 68 32549.0 69 32549.0 70 23335.5 71 14122.0 72 14122.0 73 14122.0 74 9701.0 75 5280.0 76 5280.0 77 5280.0 78 3574.0 79 1868.0 80 1868.0 81 1868.0 82 1216.0 83 564.0 84 564.0 85 564.0 86 342.0 87 120.0 88 120.0 89 120.0 90 71.0 91 22.0 92 22.0 93 22.0 94 14.5 95 7.0 96 7.0 97 7.0 98 3.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40610099221653456 2 0.0 3 0.0 4 0.0 5 0.05105707874623098 6 0.0 7 0.0 8 0.0029582427599747566 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 2.1912909333146344E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.006190396886613842 25 0.0012599922866559146 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1825408.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.32127169518296 #Duplication Level Percentage of deduplicated Percentage of total 1 84.75481639222629 61.295761037775435 2 10.821166227642346 15.6520100561612 3 2.495171780741219 5.413619888434175 4 0.8121607705446756 2.349459989869225 5 0.3479070034043796 1.2580538458932538 6 0.1944928291546679 0.8439581244035723 7 0.11819554797734806 0.5983636636901576 8 0.08000037132750046 0.46285828723933475 9 0.056459598760359456 0.3674906983574074 >10 0.2769610448929931 3.534125379612505 >50 0.01952952312158854 0.9847044933803953 >100 0.018602827747045657 2.7613122902637515 >500 0.002721649475741876 1.4264935874044908 >1k 0.0017388316095017532 2.717937762739641 >5k 7.560137432616319E-5 0.3338508947754626 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6106 0.3345005609704789 No Hit TATCAACGCAGAGTACTTTTTTTTT 4409 0.24153504312460555 No Hit GGTATCAACGCAGAGTACTTTTTTT 4318 0.23654985625131478 No Hit CTTTAGGACGTGAAATATGGCGAGG 4216 0.23096206437136246 No Hit CTGTAGGACGTGGAATATGGCAAGA 4090 0.22405949793142138 No Hit GTCCTACAGTGGACATTTCTAAATT 4088 0.2239499333847556 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4048 0.22175864245144097 No Hit CTGAAGGACCTGGAATATGGCGAGA 2233 0.12232881635228945 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1995 0.10929063529906738 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.478227333286586E-5 2 0.0 0.0 0.0 0.0 5.478227333286586E-5 3 0.0 0.0 0.0 5.478227333286586E-5 5.478227333286586E-5 4 0.0 0.0 0.0 1.6434681999859756E-4 5.478227333286586E-5 5 0.0 0.0 0.0 2.1912909333146344E-4 5.478227333286586E-5 6 0.0 0.0 0.0 2.1912909333146344E-4 5.478227333286586E-5 7 0.0 0.0 0.0 2.1912909333146344E-4 5.478227333286586E-5 8 0.0 0.0 0.0 2.1912909333146344E-4 5.478227333286586E-5 9 0.0 0.0 0.0 3.8347591333006103E-4 5.478227333286586E-5 10 0.0 0.0 0.0 4.930404599957927E-4 5.478227333286586E-5 11 0.0 0.0 0.0 6.573872799943902E-4 5.478227333286586E-5 12 0.0 0.0 0.0 6.573872799943902E-4 5.478227333286586E-5 13 0.0 0.0 0.0 6.573872799943902E-4 1.0956454666573172E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACGTA 45 6.779414E-4 14.773091 6 GGTATCA 1535 0.0 12.609615 1 TAGGACC 485 0.0 12.1468 4 GGCGAGG 1025 0.0 11.211692 19 ACTGTTC 550 0.0 10.705694 8 CCAACGA 145 1.4493708E-8 10.480008 19 GATATAC 520 0.0 10.451671 1 AGGACCT 1340 0.0 10.281941 5 AGAACCG 85 6.581799E-4 10.060869 5 TTAGGAC 1560 0.0 9.928301 3 TGTAGGA 2180 0.0 9.80704 2 GACGTGA 1385 0.0 9.805553 7 TAGGACA 650 0.0 9.794298 4 GGACCTA 175 2.586603E-9 9.768329 6 AGGACGT 2455 0.0 9.676102 5 CACTGTT 600 0.0 9.65527 7 AATGTCC 730 0.0 9.627083 8 GGACGTG 2430 0.0 9.614234 6 ACGTGAA 1515 0.0 9.591017 8 CAGGACT 240 1.8189894E-12 9.501933 4 >>END_MODULE