FastQCFastQC Report
Thu 26 May 2016
SRR1512149_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512149_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2880429
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55170.1915339694191386No Hit
TATCAACGCAGAGTACTTTTTTTTT33620.11671872488438353No Hit
GGTATCAACGCAGAGTACTTTTTTT33420.11602438386781969No Hit
GTCCTACAGTGGACATTTCTAAATT29490.10238058289234No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGC750.001898697210.5475711
CTCAACG951.0971288E-410.4087882
GGTATCA15800.010.3264391
GTATCAA31750.010.3087981
AAAGGCG1304.095873E-710.1837175
GTCTTAG3500.010.1708721
TCCAACG2250.010.08672918
ACCGCAC750.002751967610.0867288
TAGGACC5750.09.7029944
GTAGAAC7150.09.6546933
GTACTAG1251.7166565E-59.4928141
TACACCG1201.7968174E-59.4563085
TGGACCG900.00116208449.4563075
GGCGAGG8750.09.40227219
GTATAGG2950.09.38555051
TAGACTG3950.09.3366085
GTCCTAC18250.09.2652471
TGTAGGA18800.09.2571772
CCTATAC2054.620233E-109.2256673
TCTATAC3200.09.1607993
Produced by FastQC (version 0.11.5)