##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512149_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2880429 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.620013546593235 31.0 28.0 33.0 16.0 34.0 2 26.07914064189744 31.0 25.0 33.0 10.0 34.0 3 27.752054294690133 31.0 27.0 34.0 16.0 34.0 4 32.00930764132704 35.0 32.0 37.0 19.0 37.0 5 32.3948061208938 35.0 33.0 37.0 22.0 37.0 6 32.64512404228676 35.0 33.0 37.0 25.0 37.0 7 32.67925715232002 36.0 33.0 37.0 25.0 37.0 8 32.71853845382059 36.0 33.0 37.0 25.0 37.0 9 34.22829828473467 39.0 35.0 39.0 26.0 39.0 10 34.07151955489964 38.0 34.0 39.0 25.0 39.0 11 34.08358025835735 38.0 35.0 39.0 25.0 39.0 12 33.97204687218466 38.0 34.0 39.0 25.0 39.0 13 33.959013396962746 38.0 34.0 39.0 25.0 39.0 14 34.983223679528294 39.0 35.0 41.0 25.0 41.0 15 34.90707495307122 39.0 34.0 41.0 24.0 41.0 16 34.860528761514345 39.0 34.0 41.0 24.0 41.0 17 34.79386265032049 39.0 34.0 41.0 23.0 41.0 18 34.73102582983299 39.0 34.0 41.0 23.0 41.0 19 34.74644783815189 39.0 34.0 41.0 22.0 41.0 20 34.66509572011669 39.0 34.0 41.0 21.0 41.0 21 34.58946740225154 39.0 34.0 41.0 19.0 41.0 22 34.46720123981532 39.0 34.0 41.0 18.0 41.0 23 34.340125377157364 39.0 34.0 41.0 18.0 41.0 24 34.21359387785639 39.0 34.0 41.0 17.0 41.0 25 34.06787912494979 39.0 34.0 41.0 16.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0544839464576299 1101 2 3.3299527936568403 1101 3 1.8397557126276851 1101 4 1.095121516389817 1101 5 0.7814795951938365 1101 6 0.5106829077556512 1101 7 0.31167176999878166 1101 8 0.4577583757902133 1101 9 0.3771266246974747 1101 10 0.04576068626943197 1101 11 0.4684644193555272 1101 12 0.11012903082491476 1101 13 0.06372374953867421 1101 14 0.22325095565963693 1101 15 0.3614448765475444 1101 16 0.18781466471449448 1101 17 -0.0016117118845002665 1101 18 0.02585584730572066 1101 19 0.19201608092232192 1101 20 0.21467469987890553 1101 21 0.3723171815513382 1101 22 0.23895680802466757 1101 23 0.19434244388546773 1101 24 0.058062254338594244 1101 25 -0.06612299769628294 1102 1 1.2863062251420168 1102 2 3.837304871412808 1102 3 2.446997615609259 1102 4 1.4422871166413742 1102 5 1.173286469374716 1102 6 1.0777158779640672 1102 7 0.8994096643681218 1102 8 0.7567562687042226 1102 9 0.7841208839980354 1102 10 0.8227075998389779 1102 11 0.8416406183468226 1102 12 0.881094483914076 1102 13 0.8877086455595915 1102 14 0.872519386226017 1102 15 0.9190914498467393 1102 16 1.0718492786128593 1102 17 0.8013601173661442 1102 18 0.7165033907418703 1102 19 0.7145690726508107 1102 20 0.9967053097622198 1102 21 0.8330404261432776 1102 22 0.9569384135133348 1102 23 0.7743003284352312 1102 24 0.6412897867887963 1102 25 0.6186552565247823 1103 1 0.903061578554528 1103 2 2.671758890801499 1103 3 1.6723685892898672 1103 4 1.1449272890056221 1103 5 0.8252114261832801 1103 6 0.670277549789482 1103 7 0.6058030889538131 1103 8 0.3556574695264061 1103 9 0.5138380630200672 1103 10 0.39660655818792634 1103 11 0.41030921434513346 1103 12 0.34320202251193166 1103 13 0.33426218654552287 1103 14 0.4579732437500681 1103 15 0.31952055631072795 1103 16 0.37717045795303505 1103 17 0.5816025687438611 1103 18 0.48189126746149213 1103 19 0.6182167952254858 1103 20 0.5465992592009883 1103 21 0.6187866564377842 1103 22 0.5233458595917 1103 23 0.5450307546931796 1103 24 0.4073547252560914 1103 25 0.5104204120228317 1104 1 1.218301529691388 1104 2 3.8390357262867063 1104 3 2.26793752906028 1104 4 1.6515784703507705 1104 5 1.2846988843757075 1104 6 0.8924847168380055 1104 7 0.7783549843236628 1104 8 0.6944295257177728 1104 9 0.7585135878366032 1104 10 0.7861121508333966 1104 11 0.6863070280361185 1104 12 0.7085027423684451 1104 13 0.6641924715784242 1104 14 0.7763256452467289 1104 15 0.4341879972434697 1104 16 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14 -1.6024719136565224 2315 15 -1.8265694504387326 2315 16 -1.8646201824092756 2315 17 -1.7191412956760743 2315 18 -1.824415118008794 2315 19 -1.8476233480036726 2315 20 -1.8932411118280257 2315 21 -1.835502543490854 2315 22 -2.0845707638804924 2315 23 -1.7930808512201821 2315 24 -1.7146262036245261 2315 25 -1.7132162036009433 2316 1 -1.6222752792899904 2316 2 -1.94851146835801 2316 3 -1.2961884702469462 2316 4 -1.8945695640920732 2316 5 -1.90546565218704 2316 6 -2.02722931715693 2316 7 -2.004502053589686 2316 8 -2.0604949143870535 2316 9 -2.28068226286074 2316 10 -2.4677854215470347 2316 11 -2.7445594046787676 2316 12 -2.5771730931062145 2316 13 -2.6499970150795384 2316 14 -2.982156637216626 2316 15 -3.1256399415780898 2316 16 -2.966422932777956 2316 17 -2.920414884153921 2316 18 -2.804465359852312 2316 19 -2.9824058827344935 2316 20 -2.9654383812151117 2316 21 -3.0068020595843024 2316 22 -2.7852718009485358 2316 23 -2.86188204929773 2316 24 -2.8750497124024896 2316 25 -3.345282320301191 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 134833.0 3 1.0 4 1098.0 5 5638.0 6 6571.0 7 5901.0 8 5431.0 9 5480.0 10 5239.0 11 4919.0 12 4958.0 13 5016.0 14 5007.0 15 5170.0 16 5525.0 17 6035.0 18 6894.0 19 8531.0 20 10929.0 21 14319.0 22 17548.0 23 20942.0 24 24273.0 25 28088.0 26 31134.0 27 35071.0 28 41202.0 29 49750.0 30 61352.0 31 78031.0 32 98635.0 33 127999.0 34 167862.0 35 225574.0 36 317794.0 37 437094.0 38 870585.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.737825164237684 18.617608696482364 12.956299217928995 24.68826692135095 2 16.083730000842223 21.65782816915316 38.767617700615986 23.49082412938863 3 18.204475791626873 25.147816523163733 30.02625650554136 26.621451179668025 4 12.758342594106642 17.46965469379735 38.123973894166454 31.648028817929553 5 13.484727448584916 37.80561853807193 35.40399711293005 13.305656900413098 6 31.17247465568497 37.188870130109095 18.55983952390425 13.078815690301687 7 27.616650158709 32.06480701312201 22.57670645587862 17.74183637229038 8 25.18612743493221 36.35852978334055 20.960526923339973 17.49481585838727 9 26.454114994676143 15.784940368257644 20.515034392446402 37.245910244619814 10 15.36364895645753 28.893022532407496 34.063953668012644 21.679374843122325 11 33.662867579794536 22.83951453064804 24.837654391064664 18.65996349849276 12 23.20442545190317 24.802138848067422 31.5346776469755 20.458758053053902 13 28.486485867209367 22.10337418488704 26.06726289729759 23.342877050606003 14 21.391421601217324 21.989702199782602 27.707408049031724 28.911468149968357 15 23.07187575184113 30.14964090418476 23.784859824699723 22.993623519274387 16 22.504321404901837 28.071860129168257 27.234901467802192 22.18891699812771 17 21.98808649270178 28.26496617863734 27.636760925806858 22.11018640285402 18 21.73599835302311 27.607102969731244 29.177598198046194 21.479300479199452 19 23.421186201188576 27.103875751539185 27.500866192523176 21.97407185474906 20 23.470366209466093 27.310628837103373 27.934182003507814 21.28482294992272 21 23.313835066814747 26.757387012411705 27.185136092669595 22.743641828103957 22 23.30695403266543 27.49214956872534 27.370361562483186 21.830534836126045 23 22.819817852509345 27.281504423150434 27.4962305752682 22.402447149072025 24 23.033091343244617 26.953039156428872 27.94931713107216 22.064552369254358 25 22.981098642285104 27.111873652110035 27.75584045148846 22.151187254116405 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 580.0 1 580.0 2 672.0 3 764.0 4 764.0 5 764.0 6 1830.5 7 2897.0 8 2897.0 9 2897.0 10 4304.0 11 5711.0 12 5711.0 13 5711.0 14 9699.5 15 13688.0 16 13688.0 17 13688.0 18 24254.0 19 34820.0 20 34820.0 21 34820.0 22 54090.0 23 73360.0 24 73360.0 25 73360.0 26 103355.0 27 133350.0 28 133350.0 29 133350.0 30 171766.5 31 210183.0 32 210183.0 33 210183.0 34 239157.5 35 268132.0 36 268132.0 37 268132.0 38 293129.0 39 318126.0 40 318126.0 41 318126.0 42 338374.5 43 358623.0 44 358623.0 45 358623.0 46 365839.0 47 373055.0 48 373055.0 49 373055.0 50 363509.5 51 353964.0 52 353964.0 53 353964.0 54 319085.5 55 284207.0 56 284207.0 57 284207.0 58 243847.5 59 203488.0 60 203488.0 61 203488.0 62 165628.5 63 127769.0 64 127769.0 65 127769.0 66 98443.5 67 69118.0 68 69118.0 69 69118.0 70 50050.0 71 30982.0 72 30982.0 73 30982.0 74 21322.5 75 11663.0 76 11663.0 77 11663.0 78 7910.5 79 4158.0 80 4158.0 81 4158.0 82 2708.0 83 1258.0 84 1258.0 85 1258.0 86 821.0 87 384.0 88 384.0 89 384.0 90 246.0 91 108.0 92 108.0 93 108.0 94 68.5 95 29.0 96 29.0 97 29.0 98 20.5 99 12.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.367856315847397 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 3.4717050828192604E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 2.7773640662554085E-4 15 0.0 16 0.0 17 3.471705082819261E-5 18 0.0 19 2.4301935579734826E-4 20 3.471705082819261E-5 21 6.943410165638521E-5 22 4.860387115946965E-4 23 5.554728132510817E-4 24 2.7773640662554085E-4 25 3.471705082819261E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2880429.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.23295028015767 #Duplication Level Percentage of deduplicated Percentage of total 1 83.87325362386389 61.42285812471477 2 10.944320515830574 16.0296976037186 3 2.7351671573737124 6.009130813316078 4 0.9530268920255958 2.791718839974546 5 0.46874397715742505 1.7163752186646537 6 0.2880311649612901 1.2656023189647632 7 0.16986724941019102 0.8707915881198564 8 0.11201099530460899 0.6562316519978724 9 0.07532964787008595 0.4964951121382611 >10 0.35015135626576516 4.295809027411019 >50 0.016899230368391763 0.846432956379393 >100 0.010951980497076783 1.569914702124365 >500 0.001385958789033129 0.7265496173892272 >1k 8.124586000544472E-4 1.1093015133053576 >5k 4.779168235614394E-5 0.19309091178121654 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5517 0.1915339694191386 No Hit TATCAACGCAGAGTACTTTTTTTTT 3362 0.11671872488438353 No Hit GGTATCAACGCAGAGTACTTTTTTT 3342 0.11602438386781969 No Hit GTCCTACAGTGGACATTTCTAAATT 2949 0.10238058289234 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 3.471705082819261E-5 0.0 0.0 0.0 0.0 11 3.471705082819261E-5 0.0 0.0 3.471705082819261E-5 0.0 12 3.471705082819261E-5 0.0 0.0 6.943410165638521E-5 0.0 13 3.471705082819261E-5 0.0 0.0 6.943410165638521E-5 3.471705082819261E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTAGC 75 0.0018986972 10.547571 1 CTCAACG 95 1.0971288E-4 10.408788 2 GGTATCA 1580 0.0 10.326439 1 GTATCAA 3175 0.0 10.308798 1 AAAGGCG 130 4.095873E-7 10.183717 5 GTCTTAG 350 0.0 10.170872 1 TCCAACG 225 0.0 10.086729 18 ACCGCAC 75 0.0027519676 10.086728 8 TAGGACC 575 0.0 9.702994 4 GTAGAAC 715 0.0 9.654693 3 GTACTAG 125 1.7166565E-5 9.492814 1 TACACCG 120 1.7968174E-5 9.456308 5 TGGACCG 90 0.0011620844 9.456307 5 GGCGAGG 875 0.0 9.402272 19 GTATAGG 295 0.0 9.3855505 1 TAGACTG 395 0.0 9.336608 5 GTCCTAC 1825 0.0 9.265247 1 TGTAGGA 1880 0.0 9.257177 2 CCTATAC 205 4.620233E-10 9.225667 3 TCTATAC 320 0.0 9.160799 3 >>END_MODULE