FastQCFastQC Report
Thu 26 May 2016
SRR1512149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512149_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2880429
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71730.24902540559062555No Hit
GGTATCAACGCAGAGTACTTTTTTT45900.15935126330140406No Hit
TATCAACGCAGAGTACTTTTTTTTT45870.15924711214891948No Hit
GTCCTACAGTGGACATTTCTAAATT32080.11137229905684189No Hit
GTCCTAAAGTGTGTATTTCTCATTT31600.10970588061708862No Hit
CTGTAGGACGTGGAATATGGCAAGA30400.10553983451770553No Hit
CTTTAGGACGTGAAATATGGCGAGG29780.10338737736635757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19700.011.9570861
TAGGACC5900.010.9508934
GTATTAG3050.010.3183141
TTAGGAC13800.010.1211763
GTATAAA4350.010.0847041
AGGACGT20700.09.9605215
GACGTGA11150.09.8805197
TAGGACG21400.09.7679084
TTAGACT2550.09.6878164
GGACGTG20550.09.6589766
GGCGAGG8200.09.61270819
TGGACCG1304.2328284E-69.5015125
TAAGACG800.0045115029.5015114
GTCCTAA11500.09.37076951
TGTAGGA18350.09.3202842
AAGTCGT2054.2564352E-109.2655827
GTATCAA38500.09.2146081
GATATAC6950.09.1935791
GTAGGAC17500.09.1757453
GTACCGT1252.7650694E-59.1173336
Produced by FastQC (version 0.11.5)