FastQCFastQC Report
Thu 26 May 2016
SRR1512148_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512148_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064855
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68430.33140341573621396No Hit
GGTATCAACGCAGAGTACTTTTTTT44600.2159957963149955No Hit
TATCAACGCAGAGTACTTTTTTTTT44350.21478505754641367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15550.012.0765891
CGTGCGC951.4312249E-510.94993810
ACTGCGT750.00275030310.0872158
GTCTTAA1905.12955E-109.8837351
AAAGGCG1356.858918E-79.8070155
GTATCAA29650.09.5670551
GCGTGCG900.0011612799.4567649
TAGGACG1701.7935236E-89.4567644
TCGGTCA900.0011612799.45676413
CTACTAG1053.0220352E-49.413081
GTATAAT2457.2759576E-129.2786071
TGGACTG3500.09.1865715
TATACTA2107.2759576E-109.0064425
GTGTAGG3000.08.8953611
TCAGGAC3200.08.8657173
TCCTAGT1451.02499325E-58.86127952
GTCCTAA2251.1623342E-98.7855421
TAGACAG3450.08.7714915
GTCTAAG2154.780304E-98.7344641
GTGTAGC3850.08.7284931
Produced by FastQC (version 0.11.5)