##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512148_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2064855 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.12747723205746 31.0 30.0 33.0 23.0 34.0 2 26.561188073738833 31.0 25.0 34.0 10.0 34.0 3 28.247168929537427 31.0 28.0 34.0 16.0 34.0 4 32.583483586014516 35.0 32.0 37.0 25.0 37.0 5 32.976188642786056 35.0 33.0 37.0 26.0 37.0 6 33.2353608364752 36.0 35.0 37.0 27.0 37.0 7 33.27856919735284 37.0 35.0 37.0 27.0 37.0 8 33.32696775318364 37.0 35.0 37.0 27.0 37.0 9 34.87949662324957 39.0 35.0 39.0 28.0 39.0 10 34.74595552714355 39.0 35.0 39.0 27.0 39.0 11 34.76499173065421 39.0 35.0 39.0 27.0 39.0 12 34.6652026413477 39.0 35.0 39.0 27.0 39.0 13 34.65193294444404 39.0 35.0 39.0 27.0 39.0 14 35.72180564736991 40.0 36.0 41.0 27.0 41.0 15 35.63824772199501 40.0 36.0 41.0 27.0 41.0 16 35.59671841364164 40.0 36.0 41.0 27.0 41.0 17 35.53569814829613 39.0 36.0 41.0 27.0 41.0 18 35.4813093413339 39.0 36.0 41.0 27.0 41.0 19 35.502544246448295 40.0 36.0 41.0 27.0 41.0 20 35.42493395420017 39.0 36.0 41.0 27.0 41.0 21 35.355549421145795 39.0 36.0 41.0 26.0 41.0 22 35.24607054732657 39.0 35.0 41.0 26.0 41.0 23 35.126990999368 39.0 35.0 41.0 25.0 41.0 24 35.002043242745856 39.0 35.0 41.0 25.0 41.0 25 34.86485685435539 39.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6723093462271237 1101 2 3.106269901506707 1101 3 1.4526537493576086 1101 4 0.7756970662097373 1101 5 0.41773461278185664 1101 6 0.06808019243550945 1101 7 -0.06839285237911241 1101 8 -0.07521182916924118 1101 9 -0.23595312784672728 1101 10 -0.5021391753916262 1101 11 -0.0944460587430811 1101 12 -0.42634614876664045 1101 13 -0.36231095569626603 1101 14 -0.2759983010460658 1101 15 -0.25342052944923665 1101 16 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1.673122590604823 1103 4 1.0032147014843815 1103 5 0.7804080513129819 1103 6 0.7359970923026893 1103 7 0.6615777865054966 1103 8 0.4326111779061321 1103 9 0.47348979061624163 1103 10 0.36502626825383544 1103 11 0.37487816983468036 1103 12 0.38366371589985704 1103 13 0.5277637013755765 1103 14 0.6308473016086111 1103 15 0.37264962992195194 1103 16 0.3975086218846684 1103 17 0.4588429099347522 1103 18 0.521757079818137 1103 19 0.6525434625737887 1103 20 0.7820808868736506 1103 21 0.8890411352468774 1103 22 0.6161784202109359 1103 23 0.6715553476975913 1103 24 0.6416585382234601 1103 25 0.6745756235401643 1104 1 1.2722229093072848 1104 2 3.8001383921176526 1104 3 2.405474223633231 1104 4 1.6024444073349144 1104 5 1.232666402777724 1104 6 0.8637312879295109 1104 7 0.8778318585582952 1104 8 0.8176281873665161 1104 9 0.8516039643651752 1104 10 0.8768691801772874 1104 11 0.7843205239920863 1104 12 0.8584101367006838 1104 13 0.7172192784350386 1104 14 0.8843642329336632 1104 15 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0.12793802861520476 1107 15 0.12919407491478552 1107 16 0.35240660674593016 1107 17 0.245269762880163 1107 18 0.1760729783676851 1107 19 0.01551127091374127 1107 20 0.13223033019467323 1107 21 0.22131414973843988 1107 22 0.09400452560375072 1107 23 0.3030071943693713 1107 24 0.5406492657630011 1107 25 0.5271959387950105 1108 1 0.6736005064165873 1108 2 2.2555223126235227 1108 3 1.3897899417904114 1108 4 0.96260926731685 1108 5 0.8529825726060878 1108 6 0.7892823267732325 1108 7 0.6018562488021928 1108 8 0.6273933183511602 1108 9 0.6396617782766469 1108 10 0.6354057655431404 1108 11 0.610652953983049 1108 12 0.664200561271592 1108 13 0.5866546894558198 1108 14 0.6379234018586857 1108 15 0.692808712118989 1108 16 0.5773315140227453 1108 17 0.7282713822710605 1108 18 0.732389255858152 1108 19 0.779850063161561 1108 20 0.8181664426117408 1108 21 0.7326662105439539 1108 22 0.8522046509759491 1108 23 0.8835255300534328 1108 24 1.0313083839785335 1108 25 0.899653886870027 1109 1 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19 -1.4882544217532683 2315 20 -1.5275298470500616 2315 21 -1.59721822494393 2315 22 -1.7642443215075048 2315 23 -1.5852328667222082 2315 24 -1.495444157860213 2315 25 -1.5997840474816982 2316 1 -1.1493474415313578 2316 2 -1.4207039136027007 2316 3 -0.7504663859659182 2316 4 -1.1573517651441811 2316 5 -1.2121325425090284 2316 6 -1.2550298425389315 2316 7 -1.3167087447627992 2316 8 -1.4148274738696323 2316 9 -1.5561626175477485 2316 10 -1.6642081340707549 2316 11 -1.9942305009004073 2316 12 -1.9005284534574187 2316 13 -1.79758311978199 2316 14 -1.9423926284573412 2316 15 -2.3874894431791702 2316 16 -2.0949639957727655 2316 17 -1.9118115578118804 2316 18 -1.8840754231065304 2316 19 -2.1223896765781802 2316 20 -2.147817398372098 2316 21 -2.24269307731533 2316 22 -2.06814069436939 2316 23 -1.9939573099294066 2316 24 -1.9653758002056492 2316 25 -2.3739536492375066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75501.0 3 3.0 4 642.0 5 3237.0 6 3838.0 7 3424.0 8 3174.0 9 3126.0 10 3095.0 11 2946.0 12 2963.0 13 2911.0 14 3000.0 15 3230.0 16 3310.0 17 3744.0 18 4149.0 19 5097.0 20 6716.0 21 8597.0 22 10475.0 23 12758.0 24 14698.0 25 17190.0 26 19610.0 27 22572.0 28 26276.0 29 32266.0 30 40821.0 31 52520.0 32 67765.0 33 90228.0 34 120740.0 35 166217.0 36 237336.0 37 328425.0 38 662255.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.321686026379574 18.624503899789573 13.400117683808308 24.653692390022545 2 16.817139817286098 21.463796600320805 37.819368443004024 23.899695139389074 3 18.308597940291207 24.352557443500874 30.167735748999323 27.171108867208595 4 12.879790590622587 16.85653472035567 37.53406413525405 32.7296105537677 5 13.97628404900102 37.175927607507546 34.9846357250267 13.863152618464735 6 31.999583505863612 36.46638625956786 17.877817086429797 13.656213148138733 7 28.025793578725867 32.01135188669422 21.912482958851832 18.05037157572808 8 26.092900739957255 34.784771186658205 20.570656740517105 18.55167133286744 9 26.368873359146285 15.690399568008406 20.20747219538418 37.73325487746113 10 15.620370437633635 28.42180201515361 33.43450266483603 22.523324882376727 11 33.92228509992227 23.181676195180774 23.805739386058587 19.09029931883837 12 22.956381925123072 24.940056323567514 30.66801300817733 21.435548743132085 13 28.137714270493568 21.706512079540694 26.096166558910916 24.05960709105482 14 22.07382252606008 21.17341552194742 27.521158983438944 29.231602968553556 15 23.721859404171237 28.59188659736398 24.08401558462943 23.602238413835355 16 23.266524768082988 27.964627056137115 25.91465260272513 22.854195573054767 17 22.371944941385685 27.682489900012303 27.09789650987431 22.847668648727705 18 22.25904482397069 27.457085364347616 27.982400701259895 22.3014691104218 19 23.299345425241132 27.081601974378795 27.813712556638443 21.805340043741634 20 23.48860838452344 26.6292693132486 27.883451211031108 21.99867109119685 21 23.634842947733837 26.443709821614508 27.51298639032106 22.408460840330598 22 23.559079310146096 26.29376664472795 27.478796208719015 22.668357836406933 23 23.358616922150052 26.30416631958637 27.337706867928034 22.99950989033554 24 23.01082403080127 26.42288786110371 27.634065428481485 22.93222267961353 25 23.192245069142903 26.41891387962539 27.61338089763247 22.775460153599237 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 911.0 1 911.0 2 926.5 3 942.0 4 942.0 5 942.0 6 2106.5 7 3271.0 8 3271.0 9 3271.0 10 4458.0 11 5645.0 12 5645.0 13 5645.0 14 8270.0 15 10895.0 16 10895.0 17 10895.0 18 18376.0 19 25857.0 20 25857.0 21 25857.0 22 37825.5 23 49794.0 24 49794.0 25 49794.0 26 67972.0 27 86150.0 28 86150.0 29 86150.0 30 107409.5 31 128669.0 32 128669.0 33 128669.0 34 148721.5 35 168774.0 36 168774.0 37 168774.0 38 190335.0 39 211896.0 40 211896.0 41 211896.0 42 230336.0 43 248776.0 44 248776.0 45 248776.0 46 258285.5 47 267795.0 48 267795.0 49 267795.0 50 266995.0 51 266195.0 52 266195.0 53 266195.0 54 245108.0 55 224021.0 56 224021.0 57 224021.0 58 193844.0 59 163667.0 60 163667.0 61 163667.0 62 133997.5 63 104328.0 64 104328.0 65 104328.0 66 80414.0 67 56500.0 68 56500.0 69 56500.0 70 41159.0 71 25818.0 72 25818.0 73 25818.0 74 17963.0 75 10108.0 76 10108.0 77 10108.0 78 6786.0 79 3464.0 80 3464.0 81 3464.0 82 2224.0 83 984.0 84 984.0 85 984.0 86 638.5 87 293.0 88 293.0 89 293.0 90 185.5 91 78.0 92 78.0 93 78.0 94 48.5 95 19.0 96 19.0 97 19.0 98 12.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.319819067198423 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.9057730445963516E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 5.327250581759978E-4 15 0.0 16 0.0 17 4.842955074327253E-5 18 0.0 19 1.4528865222981758E-4 20 1.4528865222981758E-4 21 4.842955074327253E-5 22 5.811546089192703E-4 23 5.327250581759978E-4 24 2.4214775371636266E-4 25 4.842955074327253E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2064855.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.08899277632653 #Duplication Level Percentage of deduplicated Percentage of total 1 80.94632947582863 57.54393031377328 2 12.512158022286119 17.789534225251078 3 3.388088376380227 7.225673703421497 4 1.298978187228155 3.6937220387387213 5 0.6191054030240476 2.200578976168062 6 0.3542895435071688 1.5111652079465485 7 0.2131839471564005 1.060852245560109 8 0.1424417068578825 0.8100829975894102 9 0.09899760811214109 0.6333876223161842 >10 0.4068957537045346 4.785942211350902 >50 0.013349449685316192 0.6419188287958183 >100 0.00494706614794446 0.6252192849631628 >500 6.863667118985611E-4 0.3420397587507146 >1k 4.8045669832898647E-4 0.8020612773672159 >5k 6.863667118985521E-5 0.3338913080072916 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6843 0.33140341573621396 No Hit GGTATCAACGCAGAGTACTTTTTTT 4460 0.2159957963149955 No Hit TATCAACGCAGAGTACTTTTTTTTT 4435 0.21478505754641367 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 9.685910148654505E-5 7 0.0 0.0 0.0 0.0 9.685910148654505E-5 8 0.0 0.0 0.0 0.0 9.685910148654505E-5 9 0.0 0.0 0.0 0.0 9.685910148654505E-5 10 0.0 0.0 0.0 0.0 9.685910148654505E-5 11 0.0 0.0 0.0 0.0 9.685910148654505E-5 12 0.0 0.0 0.0 0.0 9.685910148654505E-5 13 0.0 0.0 0.0 0.0 9.685910148654505E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1555 0.0 12.076589 1 CGTGCGC 95 1.4312249E-5 10.949938 10 ACTGCGT 75 0.002750303 10.087215 8 GTCTTAA 190 5.12955E-10 9.883735 1 AAAGGCG 135 6.858918E-7 9.807015 5 GTATCAA 2965 0.0 9.567055 1 GCGTGCG 90 0.001161279 9.456764 9 TAGGACG 170 1.7935236E-8 9.456764 4 TCGGTCA 90 0.001161279 9.456764 13 CTACTAG 105 3.0220352E-4 9.41308 1 GTATAAT 245 7.2759576E-12 9.278607 1 TGGACTG 350 0.0 9.186571 5 TATACTA 210 7.2759576E-10 9.006442 5 GTGTAGG 300 0.0 8.895361 1 TCAGGAC 320 0.0 8.865717 3 TCCTAGT 145 1.02499325E-5 8.8612795 2 GTCCTAA 225 1.1623342E-9 8.785542 1 TAGACAG 345 0.0 8.771491 5 GTCTAAG 215 4.780304E-9 8.734464 1 GTGTAGC 385 0.0 8.728493 1 >>END_MODULE