FastQCFastQC Report
Thu 26 May 2016
SRR1512148_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512148_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064855
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84710.4102467243462616No Hit
TATCAACGCAGAGTACTTTTTTTTT60520.29309564109828534No Hit
GGTATCAACGCAGAGTACTTTTTTT56430.2732879548442869No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27470.13303597589176963No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21400.10363923859060321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17550.012.2261
GTACCGT951.366154E-510.9960196
TACACGG1252.2319182E-710.6431065
GTATCAA36050.010.2372831
AACTGCG951.6504344E-49.9963817
CCTACAC2600.09.8682653
GTAGGAC3100.09.5027743
ACACGGT1002.765859E-49.4965616
GTATTAG1752.509114E-89.2638141
CTAGGAC1752.6571797E-89.2312663
GGCGTGC1554.2830834E-79.1902218
GTGCTAA1909.909854E-99.034371
CTACGAC950.00181914639.0026283
CTAGACA2356.548362E-118.8962144
GTGTAGA2701.8189894E-128.8298881
ATTAGAC1401.0811607E-58.8240053
AGGACCG1401.0811607E-58.8240055
GCCTACG1106.878929E-48.6693461
GGGCGTC1000.0029147828.54690559
CTACACG1356.774982E-58.4469114
Produced by FastQC (version 0.11.5)