##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512148_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2064855 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.32653721447753 31.0 30.0 34.0 27.0 34.0 2 30.513988149288934 31.0 30.0 34.0 27.0 34.0 3 30.594968653973282 31.0 30.0 34.0 27.0 34.0 4 34.21320044264609 37.0 35.0 37.0 30.0 37.0 5 33.746941552796685 37.0 35.0 37.0 28.0 37.0 6 33.679577500599315 37.0 35.0 37.0 29.0 37.0 7 33.47801758476987 37.0 35.0 37.0 28.0 37.0 8 33.359727922783925 36.0 35.0 37.0 27.0 37.0 9 34.772642631080636 38.0 35.0 39.0 28.0 39.0 10 34.56282547684946 38.0 35.0 39.0 27.0 39.0 11 34.55419339372499 38.0 35.0 39.0 27.0 39.0 12 34.338860597959666 38.0 34.0 39.0 27.0 39.0 13 34.296455199033346 38.0 34.0 39.0 27.0 39.0 14 35.20930864394836 39.0 34.0 40.0 26.0 41.0 15 35.10528390613384 39.0 34.0 40.0 26.0 41.0 16 34.99060563574682 38.0 34.0 40.0 25.0 41.0 17 34.919920769254986 38.0 34.0 40.0 25.0 41.0 18 34.833069634429535 38.0 34.0 40.0 25.0 41.0 19 34.842221366633495 39.0 34.0 40.0 25.0 41.0 20 34.71202868966586 39.0 34.0 40.0 25.0 41.0 21 34.5817042843202 38.0 34.0 40.0 24.0 41.0 22 34.43166905182204 38.0 34.0 40.0 24.0 41.0 23 34.489291015591895 39.0 34.0 40.0 24.0 41.0 24 34.3316305503292 39.0 34.0 40.0 22.0 41.0 25 33.85883076535641 38.0 33.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.006608704244040808 1101 2 -0.06391823126293517 1101 3 -0.15317197652725767 1101 4 -0.1319889379393544 1101 5 -0.11846626178856212 1101 6 -0.2457539982702741 1101 7 -0.1725766847466943 1101 8 -0.3042900915182969 1101 9 -0.34774096361970663 1101 10 -0.26228148592738165 1101 11 -0.3464090973324545 1101 12 -0.3587327294684499 1101 13 -0.22640632948799322 1101 14 -0.3572247717167727 1101 15 -0.4209354098490081 1101 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-0.8926656294016624 2315 7 -0.9313061687830171 2315 8 -1.070445424593558 2315 9 -1.2542764698045445 2315 10 -1.2867425109728572 2315 11 -1.1939527479152616 2315 12 -1.1050090520159586 2315 13 -1.2801026818400985 2315 14 -1.5033609266197345 2315 15 -1.6277788294335167 2315 16 -1.6565210691691945 2315 17 -1.471709875238993 2315 18 -1.5959223368353008 2315 19 -1.6206192767296983 2315 20 -1.5969437802307382 2315 21 -1.4474864158596574 2315 22 -1.6920782939764223 2315 23 -1.6548215116763316 2315 24 -1.5252993719226993 2315 25 -1.8354243701088464 2316 1 -1.2063788023192252 2316 2 -1.2182004024942579 2316 3 -1.3764505048905349 2316 4 -1.2118894415686725 2316 5 -1.3312564237312685 2316 6 -1.4450931128636313 2316 7 -1.620695065068464 2316 8 -1.6304725363448078 2316 9 -1.8372046734223986 2316 10 -2.089437279184864 2316 11 -2.065169239821408 2316 12 -2.2065651535720576 2316 13 -2.0548828359305986 2316 14 -2.2177421237595496 2316 15 -2.199337849268396 2316 16 -2.3296294664155184 2316 17 -2.154932730875643 2316 18 -2.182765337471402 2316 19 -2.163210681390069 2316 20 -1.9572678302099646 2316 21 -2.2529098419727376 2316 22 -2.179703850567499 2316 23 -2.187475975365281 2316 24 -2.138187674466174 2316 25 -2.299413213408034 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44077.0 3 1.0 4 768.0 5 6802.0 6 8505.0 7 6898.0 8 5455.0 9 4912.0 10 4657.0 11 4556.0 12 4297.0 13 4153.0 14 4078.0 15 4093.0 16 4089.0 17 4405.0 18 4775.0 19 5336.0 20 6310.0 21 8301.0 22 10573.0 23 13311.0 24 16141.0 25 19148.0 26 21905.0 27 24929.0 28 29237.0 29 35534.0 30 45153.0 31 57232.0 32 72931.0 33 94097.0 34 120054.0 35 157687.0 36 224975.0 37 375603.0 38 609877.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.94065991772909 19.87100193521409 14.261458120605655 23.926880026451165 2 16.117451346462587 21.29641064384666 38.2276721609992 24.358465848691555 3 18.600240694867193 25.10704141453032 30.60277840332614 25.689939487276348 4 13.688176651629291 17.80890183572212 37.02351981131847 31.47940170133012 5 13.974647405504385 37.53024194036844 34.91672776027208 13.578382893855089 6 32.08065457380785 36.992524898842774 17.58099236992428 13.345828157425096 7 28.007729356298626 32.249722135452615 21.844003574100846 17.89854493414792 8 26.22841254766202 34.99851559401181 20.361400794944203 18.411671063381956 9 26.44471403561025 15.524770504466415 20.086107741221536 37.9444077187018 10 15.641195144453244 28.83045056432534 33.370575657854914 22.157778633366508 11 34.23717403885503 23.141576527165345 23.575941167781757 19.045308266197868 12 22.94127190529117 25.14893297592325 30.65367786115732 21.25611725762826 13 28.375939230599727 21.76356209031627 26.083284298413208 23.7772143806708 14 22.0955466606614 21.219601376367834 27.570023076680926 29.114828886289835 15 23.817701485092176 28.823331420366078 24.000523039148028 23.358444055393722 16 23.33577902564587 28.04371251250088 25.87339062549186 22.74711783636139 17 22.43959018914161 27.817013785471616 27.11924081836255 22.62415520702422 18 22.356775657370616 27.4032317039211 28.039692859789184 22.2002997789191 19 23.407585628413077 27.153035665510167 27.849889483604635 21.589489222472118 20 23.60974499420056 26.643226764106924 27.871497030057803 21.875531211634716 21 23.709413009630218 26.42166156945645 27.588862171920063 22.28006324899327 22 23.60751723486637 26.284266933997785 27.590411917543843 22.517803913591997 23 23.37602398231353 26.34276983129566 27.49040489525899 22.790801291131825 24 23.12771308640564 26.454006056875347 27.737980327989536 22.680300528729475 25 23.349630113932175 26.46703715811267 27.730727785647698 22.45260494230746 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 940.0 1 940.0 2 961.5 3 983.0 4 983.0 5 983.0 6 2188.0 7 3393.0 8 3393.0 9 3393.0 10 4597.0 11 5801.0 12 5801.0 13 5801.0 14 8495.0 15 11189.0 16 11189.0 17 11189.0 18 18809.0 19 26429.0 20 26429.0 21 26429.0 22 38894.0 23 51359.0 24 51359.0 25 51359.0 26 70485.0 27 89611.0 28 89611.0 29 89611.0 30 110128.0 31 130645.0 32 130645.0 33 130645.0 34 151166.0 35 171687.0 36 171687.0 37 171687.0 38 191722.0 39 211757.0 40 211757.0 41 211757.0 42 230025.5 43 248294.0 44 248294.0 45 248294.0 46 257592.0 47 266890.0 48 266890.0 49 266890.0 50 266318.5 51 265747.0 52 265747.0 53 265747.0 54 244022.0 55 222297.0 56 222297.0 57 222297.0 58 191878.5 59 161460.0 60 161460.0 61 161460.0 62 131976.0 63 102492.0 64 102492.0 65 102492.0 66 78876.5 67 55261.0 68 55261.0 69 55261.0 70 39991.5 71 24722.0 72 24722.0 73 24722.0 74 17087.5 75 9453.0 76 9453.0 77 9453.0 78 6362.5 79 3272.0 80 3272.0 81 3272.0 82 2084.0 83 896.0 84 896.0 85 896.0 86 568.5 87 241.0 88 241.0 89 241.0 90 134.5 91 28.0 92 28.0 93 28.0 94 18.0 95 8.0 96 8.0 97 8.0 98 4.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39881735037084926 2 0.0 3 0.0 4 0.0 5 0.052400773904220876 6 0.0 7 0.0 8 0.0027120548416232617 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.4528865222981758E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004552377769867618 25 0.0014528865222981759 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2064855.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.54844741902365 #Duplication Level Percentage of deduplicated Percentage of total 1 80.312928087359 55.85639456151515 2 12.697972186044524 17.662485018186846 3 3.5763525103892477 7.461892935620995 4 1.3797453630444265 3.8383659133334804 5 0.6824578528423569 2.3731942047053223 6 0.37501278349811706 1.5648934112748296 7 0.22288336124712066 1.0850834211189435 8 0.15947763006117693 0.8873137255056214 9 0.11335871376858535 0.709553028962035 >10 0.4564122887674743 5.2356364564839675 >50 0.01663334570652971 0.7925786127740624 >100 0.00551130306315487 0.7140964223332806 >500 6.272871041381047E-4 0.3463486685082062 >1k 4.181914027587289E-4 0.49463146444474343 >5k 2.0909570137936446E-4 0.977532155232526 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8471 0.4102467243462616 No Hit TATCAACGCAGAGTACTTTTTTTTT 6052 0.29309564109828534 No Hit GGTATCAACGCAGAGTACTTTTTTT 5643 0.2732879548442869 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2747 0.13303597589176963 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2140 0.10363923859060321 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 4.842955074327253E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1755 0.0 12.226 1 GTACCGT 95 1.366154E-5 10.996019 6 TACACGG 125 2.2319182E-7 10.643106 5 GTATCAA 3605 0.0 10.237283 1 AACTGCG 95 1.6504344E-4 9.996381 7 CCTACAC 260 0.0 9.868265 3 GTAGGAC 310 0.0 9.502774 3 ACACGGT 100 2.765859E-4 9.496561 6 GTATTAG 175 2.509114E-8 9.263814 1 CTAGGAC 175 2.6571797E-8 9.231266 3 GGCGTGC 155 4.2830834E-7 9.190221 8 GTGCTAA 190 9.909854E-9 9.03437 1 CTACGAC 95 0.0018191463 9.002628 3 CTAGACA 235 6.548362E-11 8.896214 4 GTGTAGA 270 1.8189894E-12 8.829888 1 ATTAGAC 140 1.0811607E-5 8.824005 3 AGGACCG 140 1.0811607E-5 8.824005 5 GCCTACG 110 6.878929E-4 8.669346 1 GGGCGTC 100 0.002914782 8.5469055 9 CTACACG 135 6.774982E-5 8.446911 4 >>END_MODULE