FastQCFastQC Report
Thu 26 May 2016
SRR1512147_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512147_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2386559
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65840.2758783671386293No Hit
GGTATCAACGCAGAGTACTTTTTTT40320.16894616894030273No Hit
TATCAACGCAGAGTACTTTTTTTTT39750.166557793040105No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGG400.005437992514.18688110
TCCAACG957.8325684E-812.94268818
GGTATCA14900.012.0591891
AAGGCGT951.0978329E-611.9468476
CTAGGAC2900.011.7408663
GGACCGT1002.0366952E-611.3495046
GGACCGA803.9301885E-410.6401616
GTCTTAG3000.010.201721
GGACGTG15200.010.1423756
TGGACCG1401.0344411E-710.1334865
GCACTCG750.002747307510.0886613
GACGTGG8200.010.0346237
AGGACGT15850.010.0247985
TTACACG856.870377E-410.0142684
CGTGCGC951.7199863E-49.95570610
GTCCTAC13150.09.91017151
CCCTATA907.83025E-49.8726332
AGGACCG1252.7212027E-69.8362375
TAGGACA5900.09.7785284
TAGGACG16750.09.7684794
Produced by FastQC (version 0.11.5)