FastQCFastQC Report
Thu 26 May 2016
SRR1512147_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512147_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2386559
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85030.3562870224452863No Hit
TATCAACGCAGAGTACTTTTTTTTT54800.22961929707164164No Hit
GGTATCAACGCAGAGTACTTTTTTT53660.22484254527124617No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25070.10504663827711781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGG400.005292343414.24506710
GGTATCA18100.012.4348161
TACACCG701.08956476E-412.2164555
CGTGCGC1201.2823693E-711.07949610
TCCAACG1303.283276E-810.95820218
CCGTGCA909.552529E-510.5519019
GCGTGCG1355.720358E-810.5519019
GTTCCGG1002.299405E-510.4905591
GTAGGAC15150.010.2229393
TGTAGGA16300.09.9680282
TAGGACC6550.09.86434654
CTAGACA3100.09.8081944
AGGACGT14750.09.791575
GATATAC3800.09.7878421
GGACGTG13700.09.7746026
GTATCAA35800.09.590151
CTGTAGG16650.09.565511
CCAACGA1106.861619E-59.49710819
GGCGAGG7000.09.49710819
GCGCCAC1502.6628004E-79.4967113
Produced by FastQC (version 0.11.5)