##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512147_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2386559 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.266490792810906 31.0 30.0 34.0 26.0 34.0 2 30.460612957819187 31.0 30.0 34.0 27.0 34.0 3 30.544125244756152 31.0 30.0 34.0 27.0 34.0 4 34.148160175382216 37.0 35.0 37.0 30.0 37.0 5 33.692079684600294 37.0 35.0 37.0 28.0 37.0 6 33.62895407153144 37.0 35.0 37.0 28.0 37.0 7 33.42767851119541 36.0 35.0 37.0 28.0 37.0 8 33.314152300445954 36.0 35.0 37.0 27.0 37.0 9 34.71838911168758 38.0 35.0 39.0 27.0 39.0 10 34.48700912066285 38.0 35.0 39.0 27.0 39.0 11 34.47520300147618 38.0 35.0 39.0 27.0 39.0 12 34.26495636604836 38.0 34.0 39.0 27.0 39.0 13 34.22978690239797 38.0 34.0 39.0 27.0 39.0 14 35.12986856809322 39.0 34.0 40.0 26.0 41.0 15 35.04242426020056 38.0 34.0 40.0 26.0 41.0 16 34.91930013043884 38.0 34.0 40.0 25.0 41.0 17 34.84276357718372 38.0 34.0 40.0 25.0 41.0 18 34.76248355896502 38.0 34.0 40.0 25.0 41.0 19 34.77674802927562 39.0 34.0 40.0 25.0 41.0 20 34.64593249108864 39.0 34.0 40.0 24.0 41.0 21 34.512237493395304 38.0 34.0 40.0 24.0 41.0 22 34.370354975510764 38.0 34.0 40.0 23.0 41.0 23 34.421723493950914 39.0 34.0 40.0 23.0 41.0 24 34.27121684400009 39.0 34.0 40.0 20.0 41.0 25 33.796868210674866 38.0 33.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21632726290465953 1101 2 0.10315792779863031 1101 3 0.12020077140657648 1101 4 0.09668060978258097 1101 5 0.2559239272973315 1101 6 0.045845962472178314 1101 7 -0.03424271058300121 1101 8 0.049808081260103165 1101 9 -0.07811477237083153 1101 10 0.10400182818188597 1101 11 0.06622149829110668 1101 12 -0.0031633584134880266 1101 13 0.20054894041419402 1101 14 -0.02244703312801022 1101 15 0.062048032061049696 1101 16 -0.11504082808041716 1101 17 0.07781994409116066 1101 18 0.12019482765026623 1101 19 -0.12397577546740735 1101 20 -0.056736484280889954 1101 21 -0.09389406559844105 1101 22 -0.23631124724631292 1101 23 -0.22342384311718178 1101 24 -0.5594805800683957 1101 25 -0.4933637745413577 1102 1 0.21460845505841064 1102 2 0.22845235152976784 1102 3 0.27284963758370395 1102 4 0.3685765528567728 1102 5 0.18072380661274678 1102 6 0.16850041103985092 1102 7 0.4214439671804229 1102 8 0.29040911378371703 1102 9 0.438631670494928 1102 10 0.4636874215331517 1102 11 0.42945912447791557 1102 12 0.5011644518027296 1102 13 0.5792959196558627 1102 14 0.5563943949145411 1102 15 0.35048927703616783 1102 16 0.36667137709502384 1102 17 0.6013053380505156 1102 18 0.5283809005332571 1102 19 0.5705555952353905 1102 20 0.6896721292165395 1102 21 0.8225989105007656 1102 22 0.922743157012043 1102 23 0.9501914179433868 1102 24 0.8338166887871168 1102 25 0.908329307666996 1103 1 0.14550985206460254 1103 2 0.18836409549228605 1103 3 0.3331258026495796 1103 4 0.41479577184888683 1103 5 0.3690656730045774 1103 6 0.396301062847094 1103 7 0.34401450781461307 1103 8 0.5170820300143504 1103 9 0.3656373496249614 1103 10 0.30316115665599597 1103 11 0.21468986881505003 1103 12 0.28463218593712725 1103 13 0.40362700818802466 1103 14 0.5957230299358969 1103 15 0.45841842597570803 1103 16 0.5223323036603063 1103 17 0.5650959155137443 1103 18 0.5615632711347445 1103 19 0.4378320927124122 1103 20 0.5169980838432977 1103 21 0.6683297137573376 1103 22 0.491067013403395 1103 23 0.4870044569023264 1103 24 0.777471945016309 1103 25 0.4409645189767133 1104 1 0.17569914111693308 1104 2 0.12199325666313499 1104 3 0.30906766199741753 1104 4 0.23275508387445853 1104 5 0.17511034417271532 1104 6 0.23491445720947723 1104 7 0.1381585142436279 1104 8 0.06530662623556083 1104 9 0.2725598957243136 1104 10 0.18519835060084233 1104 11 0.28604494083573684 1104 12 0.40842652735622664 1104 13 0.21548574457223424 1104 14 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-0.7406565894598174 2315 2 -0.7871592578991766 2315 3 -0.942769240127447 2315 4 -0.8834808665726612 2315 5 -0.946561655170953 2315 6 -0.9107053639407425 2315 7 -0.8501538985876138 2315 8 -0.9781965323500472 2315 9 -1.0574573329244643 2315 10 -1.0474802940787882 2315 11 -1.0667600472682892 2315 12 -1.1027885025888011 2315 13 -1.1181823167945737 2315 14 -1.2773720619630708 2315 15 -1.4214006415260343 2315 16 -1.3066209895567695 2315 17 -1.375744069749672 2315 18 -1.451898597955271 2315 19 -1.3902214502078891 2315 20 -1.5390778914319867 2315 21 -1.3027521970863276 2315 22 -1.4021705321367435 2315 23 -1.3113131164735847 2315 24 -1.2033156435055332 2315 25 -1.3291423212541602 2316 1 -1.5066470521101323 2316 2 -1.4425682976480054 2316 3 -1.5731252616901514 2316 4 -1.4964170077965235 2316 5 -1.6693052722552508 2316 6 -1.7386960043056483 2316 7 -1.7804610486379673 2316 8 -1.885634480338915 2316 9 -2.2516478429653404 2316 10 -2.218699119977728 2316 11 -2.3608593304861145 2316 12 -2.20031678882777 2316 13 -2.2682477750020276 2316 14 -2.3560708949705997 2316 15 -2.5199907724424477 2316 16 -2.616633725769084 2316 17 -2.572729141724082 2316 18 -2.368211908778086 2316 19 -2.133266589713841 2316 20 -2.3026111536794858 2316 21 -2.4400624867611143 2316 22 -2.392424367809987 2316 23 -2.5772718866886173 2316 24 -2.4582655872292456 2316 25 -2.666872017362209 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52644.0 3 1.0 4 877.0 5 8003.0 6 9875.0 7 7955.0 8 6533.0 9 5886.0 10 5637.0 11 5398.0 12 4888.0 13 4762.0 14 4768.0 15 4612.0 16 4729.0 17 5191.0 18 5383.0 19 6098.0 20 7536.0 21 9590.0 22 12250.0 23 15432.0 24 18946.0 25 22280.0 26 25791.0 27 29165.0 28 34122.0 29 41060.0 30 52352.0 31 67011.0 32 84353.0 33 108766.0 34 140326.0 35 184571.0 36 265323.0 37 441474.0 38 682971.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.16352623334395 20.494688198582146 14.361996878851208 23.979788689222694 2 15.407454833507153 21.710001722144728 39.77446189262449 23.108081551723632 3 18.551563150125347 25.780380874723818 30.76081504794141 24.907240927209426 4 13.201517331019264 18.38542437040107 38.07586571293649 30.33719258564318 5 13.292348403763604 38.06793956399849 35.68166582608991 12.95804620614799 6 31.065772939198233 38.07284881706256 18.12085936278969 12.740518880949518 7 27.448011970372406 32.315186844322724 22.57358816605833 17.663213019246538 8 25.155072720652043 36.67254566489878 20.739790007043904 17.432591607405275 9 26.292582751987275 15.6284843576044 20.643026214730078 37.43590667567825 10 14.952406372522113 29.47607832029294 34.316939158009504 21.254576149175445 11 33.70400647962192 23.13213291605194 24.786313684262574 18.377546920063573 12 22.968885328206845 25.244169534463634 31.78580542111048 20.00113971621904 13 28.423433068279476 22.28727636735568 26.29731760245609 22.991972961908758 14 21.146051700376987 22.23531871619348 28.13414627503447 28.48448330839506 15 22.925726956676957 30.56714709336748 24.031796406458 22.475329543497562 16 22.205191658785726 28.718041330635447 27.380634629187885 21.69613238139095 17 21.534477044145987 28.73723214050019 28.02583133289393 21.702459482459894 18 21.448411709075703 27.94881668544545 29.552673954425597 21.050097651053253 19 23.05962103565932 27.39125862068243 28.338774225798925 21.210346117859327 20 23.207890523552948 27.448011970372406 28.494120614659014 20.849976891415633 21 22.931928353751154 26.99128745612407 27.952755410614195 22.124028779510585 22 22.913617471849637 27.64754611136787 28.046698196021975 21.39213822076052 23 22.376400499631476 27.571453293214205 28.22616159918946 21.82598460796486 24 22.588168938067298 27.201284941039123 28.644413184138706 21.56613293675487 25 22.62063989299919 27.381078086790662 28.43830608868798 21.559975931522164 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 658.0 1 658.0 2 778.0 3 898.0 4 898.0 5 898.0 6 2046.0 7 3194.0 8 3194.0 9 3194.0 10 4644.0 11 6094.0 12 6094.0 13 6094.0 14 9668.0 15 13242.0 16 13242.0 17 13242.0 18 23204.0 19 33166.0 20 33166.0 21 33166.0 22 50768.0 23 68370.0 24 68370.0 25 68370.0 26 95409.0 27 122448.0 28 122448.0 29 122448.0 30 153985.5 31 185523.0 32 185523.0 33 185523.0 34 209158.0 35 232793.0 36 232793.0 37 232793.0 38 251186.0 39 269579.0 40 269579.0 41 269579.0 42 283979.0 43 298379.0 44 298379.0 45 298379.0 46 301865.0 47 305351.0 48 305351.0 49 305351.0 50 295729.5 51 286108.0 52 286108.0 53 286108.0 54 255260.0 55 224412.0 56 224412.0 57 224412.0 58 190144.5 59 155877.0 60 155877.0 61 155877.0 62 125950.0 63 96023.0 64 96023.0 65 96023.0 66 73403.0 67 50783.0 68 50783.0 69 50783.0 70 36436.0 71 22089.0 72 22089.0 73 22089.0 74 15099.0 75 8109.0 76 8109.0 77 8109.0 78 5351.5 79 2594.0 80 2594.0 81 2594.0 82 1616.5 83 639.0 84 639.0 85 639.0 86 415.5 87 192.0 88 192.0 89 192.0 90 112.5 91 33.0 92 33.0 93 33.0 94 19.0 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4135661427184495 2 0.0 3 0.0 4 0.0 5 0.054136520404481935 6 0.0 7 0.0 8 0.004106330495076803 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 8.380266316483272E-5 20 0.0 21 0.0 22 0.0 23 0.0 24 0.006117594411032787 25 0.0014665466053845725 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2386559.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.29830685426408 #Duplication Level Percentage of deduplicated Percentage of total 1 80.85423920957756 56.839161184229546 2 12.625051773301587 17.75039527221052 3 3.5021132607119796 7.385778979197542 4 1.315864094562972 3.700120715923847 5 0.6165631495389221 2.1671672740659327 6 0.32152046039933313 1.3561406391045951 7 0.1864993863145947 0.9177413761092722 8 0.12256917549717172 0.689312440797946 9 0.08397498683597113 0.5312969453410097 >10 0.34188938027027554 3.9960373684511574 >50 0.016595798903193983 0.8021612789610508 >100 0.010678131196450662 1.4552578802854506 >500 0.0016671561200385055 0.8206807631329728 >1k 5.95412900009818E-4 0.7788661008977196 >5k 1.786238700029454E-4 0.8098817812913788 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8503 0.3562870224452863 No Hit TATCAACGCAGAGTACTTTTTTTTT 5480 0.22961929707164164 No Hit GGTATCAACGCAGAGTACTTTTTTT 5366 0.22484254527124617 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2507 0.10504663827711781 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.190133158241636E-5 0.0 4 0.0 0.0 0.0 4.190133158241636E-5 0.0 5 0.0 0.0 0.0 4.190133158241636E-5 0.0 6 0.0 0.0 0.0 8.380266316483272E-5 0.0 7 0.0 0.0 0.0 8.380266316483272E-5 0.0 8 0.0 0.0 0.0 1.2570399474724908E-4 0.0 9 0.0 0.0 0.0 1.2570399474724908E-4 0.0 10 0.0 0.0 0.0 1.6760532632966544E-4 0.0 11 0.0 0.0 0.0 1.6760532632966544E-4 0.0 12 0.0 8.380266316483272E-5 0.0 1.6760532632966544E-4 4.190133158241636E-5 13 0.0 8.380266316483272E-5 0.0 1.6760532632966544E-4 8.380266316483272E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCCGG 40 0.0052923434 14.245067 10 GGTATCA 1810 0.0 12.434816 1 TACACCG 70 1.08956476E-4 12.216455 5 CGTGCGC 120 1.2823693E-7 11.079496 10 TCCAACG 130 3.283276E-8 10.958202 18 CCGTGCA 90 9.552529E-5 10.551901 9 GCGTGCG 135 5.720358E-8 10.551901 9 GTTCCGG 100 2.299405E-5 10.490559 1 GTAGGAC 1515 0.0 10.222939 3 TGTAGGA 1630 0.0 9.968028 2 TAGGACC 655 0.0 9.8643465 4 CTAGACA 310 0.0 9.808194 4 AGGACGT 1475 0.0 9.79157 5 GATATAC 380 0.0 9.787842 1 GGACGTG 1370 0.0 9.774602 6 GTATCAA 3580 0.0 9.59015 1 CTGTAGG 1665 0.0 9.56551 1 CCAACGA 110 6.861619E-5 9.497108 19 GGCGAGG 700 0.0 9.497108 19 GCGCCAC 150 2.6628004E-7 9.49671 13 >>END_MODULE