FastQCFastQC Report
Thu 26 May 2016
SRR1512146_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512146_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2211473
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59240.26787575520931073No Hit
GGTATCAACGCAGAGTACTTTTTTT36680.16586230082845235No Hit
TATCAACGCAGAGTACTTTTTTTTT35500.16052649071455993No Hit
GTCCTACAGTGGACATTTCTAAATT30560.1381884382038578No Hit
GTCCTAAAGTGTGTATTTCTCATTT29250.13226478460284163No Hit
CTTTAGGACGTGAAATATGGCGAGG27720.12534631894669301No Hit
CTGTAGGACGTGGAATATGGCAAGA27500.12435150689156052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.011.7726931
AACACCG855.5737233E-511.1264775
GGCGAGG8500.010.45888819
GTATAAG2251.8189894E-1210.0958961
ACTGCGT750.002748134810.0882338
TAGGACC6250.09.9871264
TCCAACG1804.274625E-109.98292218
CGGTAGG907.802238E-49.8764191
TCGAACT1651.1003067E-89.74409619
TGGCGAG18400.09.66310318
AATGTCC5000.09.6468738
TAGGACA6550.09.52974
GTCCTAA10800.09.5106261
GTCCTAT2501.8189894E-129.4813621
CTTATAC2002.8921932E-109.4575053
GTATCAA24200.09.4274911
GGCCTAC1905.4333213E-99.3566071
GACGTGG8750.09.2953777
GGACGTG18000.09.2473386
TGTCCAC6650.09.24438710
Produced by FastQC (version 0.11.5)