##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512146_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2211473 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.055054707880224 31.0 30.0 33.0 22.0 34.0 2 26.52479772531702 31.0 25.0 34.0 10.0 34.0 3 28.20397083753679 31.0 27.0 34.0 16.0 34.0 4 32.4978233964421 35.0 32.0 37.0 23.0 37.0 5 32.8983008157911 35.0 33.0 37.0 26.0 37.0 6 33.16320977013963 36.0 35.0 37.0 27.0 37.0 7 33.21243623593867 37.0 35.0 37.0 27.0 37.0 8 33.25429928378054 37.0 35.0 37.0 27.0 37.0 9 34.81395296257291 39.0 35.0 39.0 28.0 39.0 10 34.659605611282615 39.0 35.0 39.0 27.0 39.0 11 34.68108224699103 39.0 35.0 39.0 27.0 39.0 12 34.584571912024245 39.0 35.0 39.0 27.0 39.0 13 34.57182384772502 39.0 35.0 39.0 27.0 39.0 14 35.63539731210827 40.0 36.0 41.0 27.0 41.0 15 35.562363637268014 40.0 36.0 41.0 27.0 41.0 16 35.53154164667622 40.0 36.0 41.0 27.0 41.0 17 35.4737276919049 39.0 36.0 41.0 27.0 41.0 18 35.424703806015266 39.0 36.0 41.0 27.0 41.0 19 35.44990465630826 40.0 36.0 41.0 27.0 41.0 20 35.376984028292455 40.0 36.0 41.0 26.0 41.0 21 35.30460014659912 39.0 36.0 41.0 26.0 41.0 22 35.19047621200892 39.0 35.0 41.0 25.0 41.0 23 35.07778028490513 39.0 35.0 41.0 25.0 41.0 24 34.96452454992668 39.0 35.0 41.0 25.0 41.0 25 34.833462131348654 39.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7079083282991405 1101 2 3.0657270800039953 1101 3 1.5606553263896643 1101 4 0.9339901396092998 1101 5 0.7346094586714713 1101 6 0.3304099419660531 1101 7 0.2939885708523633 1101 8 0.35938829637424163 1101 9 0.21339384551112772 1101 10 0.030964099088500063 1101 11 0.15321166176531875 1101 12 0.08221639560304084 1101 13 0.007352267346334429 1101 14 0.05651454587999183 1101 15 0.1258807070516781 1101 16 0.10522930553197796 1101 17 0.09672697038144662 1101 18 -0.1812194998128902 1101 19 -0.2749410253704596 1101 20 -0.1385033380036731 1101 21 -0.27404102661547114 1101 22 -0.2659433251590073 1101 23 -0.22116506184681128 1101 24 -0.3904978684569471 1101 25 -0.5752884946732948 1102 1 1.0767248614110976 1102 2 3.763308731097201 1102 3 2.2199715727471414 1102 4 1.300463418633555 1102 5 0.9526501704140387 1102 6 0.904519867684499 1102 7 0.7491364738480186 1102 8 0.7087479836587178 1102 9 0.7239395450917101 1102 10 0.5732148101656733 1102 11 0.6902526301610692 1102 12 0.6239459845460473 1102 13 0.7213138834375385 1102 14 0.6360507323939757 1102 15 0.5769973596182609 1102 16 0.733564661461358 1102 17 0.4923661899445477 1102 18 0.5667179275083143 1102 19 0.7153023515516637 1102 20 0.7006441932145435 1102 21 0.5896505755446455 1102 22 0.6944744174970694 1102 23 0.7609703982224971 1102 24 0.6889011195057009 1102 25 0.5455533277543907 1103 1 0.6645441655801392 1103 2 2.6039248141694706 1103 3 1.639505866530527 1103 4 0.7673391382808816 1103 5 0.5064600990704946 1103 6 0.5128390719758258 1103 7 0.3376315765921376 1103 8 0.0983335804808192 1103 9 0.1533028612265923 1103 10 0.08647641831049668 1103 11 0.11019150987419835 1103 12 0.12231464860452235 1103 13 0.1770110136179852 1103 14 0.23676055435180388 1103 15 0.09591667466828824 1103 16 0.08170811346239759 1103 17 0.02070872339545815 1103 18 0.08048501431098032 1103 19 0.13447509614243103 1103 20 0.3399687275651644 1103 21 0.3173681018904375 1103 22 0.3465613744424658 1103 23 0.30375723807549093 1103 24 0.1567281134464551 1103 25 0.3270970230348027 1104 1 1.3882919992438083 1104 2 3.9241982911911464 1104 3 2.3365810332147454 1104 4 1.6817385325046175 1104 5 1.3034257121716024 1104 6 0.8949556663514926 1104 7 0.8109409565491816 1104 8 0.8685279842316476 1104 9 0.9847092324097702 1104 10 0.9454391461548184 1104 11 0.7721197646449269 1104 12 0.8275077916513425 1104 13 0.8155707630091769 1104 14 0.9818849012592494 1104 15 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-1.7613384796418003 2315 20 -1.8167630416574951 2315 21 -1.7654040025118931 2315 22 -1.9383482104313856 2315 23 -1.9631023462638524 2315 24 -1.9200195610804727 2315 25 -1.9277014843253113 2316 1 -1.3764768127036469 2316 2 -1.7637749539098024 2316 3 -1.1459674625860679 2316 4 -1.4796827887695123 2316 5 -1.4995882880262776 2316 6 -1.525019350963241 2316 7 -1.4783867662288586 2316 8 -1.6550793438286924 2316 9 -1.8086147014974259 2316 10 -1.9511711676055867 2316 11 -2.2482577838217708 2316 12 -2.1801310608620597 2316 13 -2.0470692098692567 2316 14 -2.245305121380852 2316 15 -2.5555038397740475 2316 16 -2.3783382706826544 2316 17 -2.4220490248651387 2316 18 -2.2627840137303465 2316 19 -2.4446151421034017 2316 20 -2.5111803342100814 2316 21 -2.65714697295671 2316 22 -2.3563033736072896 2316 23 -2.2375009318591523 2316 24 -2.15221401179074 2316 25 -2.8096307863685297 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 84787.0 3 2.0 4 707.0 5 3441.0 6 4172.0 7 3772.0 8 3580.0 9 3537.0 10 3316.0 11 3334.0 12 3169.0 13 3210.0 14 3284.0 15 3444.0 16 3621.0 17 3936.0 18 4542.0 19 5620.0 20 7082.0 21 9301.0 22 11573.0 23 13639.0 24 16041.0 25 18423.0 26 20960.0 27 24027.0 28 28133.0 29 34438.0 30 43389.0 31 55555.0 32 71825.0 33 95142.0 34 127425.0 35 175721.0 36 251744.0 37 351903.0 38 713678.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.25825954013456 19.547876008434198 13.218881713681332 24.974982737749908 2 15.978224476968578 21.98368252466109 39.60390195158969 22.434191046780636 3 18.159570566767037 25.443132247149297 30.34081808821541 26.05647909786825 4 12.317084585703737 17.753280279704974 38.80630692755462 31.123328207036664 5 13.014312180162271 38.03333796071668 35.91366478360803 13.038685075513017 6 30.3889308167 37.70030201589619 18.980064418602442 12.930702748801364 7 26.976137624108453 32.16801652111511 23.241070544383767 17.614775310392666 8 24.47994834188951 36.878211392186984 21.41152712028191 17.230313145641595 9 26.1459669641004 15.845502070339542 21.11031877847932 36.89821218708074 10 15.147415320015211 29.084641774961757 34.61412370849655 21.153819196526477 11 32.97399516069154 23.028406858234305 25.58946005671333 18.40813792436082 12 22.965236292733398 25.155857656864907 32.09363171062907 19.78527433977263 13 28.227837283114017 22.665210020651394 26.29310871080045 22.813843985434143 14 20.830561794501115 22.622856230728292 28.274263192713256 28.272318782057344 15 22.513546400973468 31.052967863500935 24.106602251078808 22.326883484446792 16 21.658279345938205 28.47531939119311 28.20947847882384 21.656922784044845 17 21.304814169024073 28.772329018861647 28.311911704059554 21.610945108054725 18 20.996277141977316 27.847773859323627 30.297995951114938 20.85795304758412 19 22.987939211309794 27.26148831724143 28.202358256792788 21.548214214655992 20 22.985601865187228 27.71169544921991 28.695748116521745 20.606954569071117 21 22.54655163603921 27.130563439957783 27.891189069347117 22.431695854655885 22 22.754389749029936 28.187228431546774 27.96863970799368 21.089742111429604 23 21.953616121129137 28.018121981064077 28.325385334019458 21.702876563787328 24 22.455124749939408 27.276817529021496 28.89334847865486 21.37470924238423 25 22.31251401781257 27.63679576318398 28.56956814284784 21.48112207615561 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1086.0 1 1086.0 2 990.5 3 895.0 4 895.0 5 895.0 6 1987.0 7 3079.0 8 3079.0 9 3079.0 10 4520.0 11 5961.0 12 5961.0 13 5961.0 14 9440.5 15 12920.0 16 12920.0 17 12920.0 18 22086.5 19 31253.0 20 31253.0 21 31253.0 22 47828.5 23 64404.0 24 64404.0 25 64404.0 26 90190.0 27 115976.0 28 115976.0 29 115976.0 30 147890.0 31 179804.0 32 179804.0 33 179804.0 34 201612.0 35 223420.0 36 223420.0 37 223420.0 38 239034.5 39 254649.0 40 254649.0 41 254649.0 42 266213.0 43 277777.0 44 277777.0 45 277777.0 46 279386.0 47 280995.0 48 280995.0 49 280995.0 50 269556.5 51 258118.0 52 258118.0 53 258118.0 54 228709.0 55 199300.0 56 199300.0 57 199300.0 58 169306.5 59 139313.0 60 139313.0 61 139313.0 62 112354.5 63 85396.0 64 85396.0 65 85396.0 66 65519.5 67 45643.0 68 45643.0 69 45643.0 70 32862.5 71 20082.0 72 20082.0 73 20082.0 74 13896.5 75 7711.0 76 7711.0 77 7711.0 78 5129.5 79 2548.0 80 2548.0 81 2548.0 82 1665.0 83 782.0 84 782.0 85 782.0 86 527.5 87 273.0 88 273.0 89 273.0 90 167.0 91 61.0 92 61.0 93 61.0 94 40.0 95 19.0 96 19.0 97 19.0 98 13.5 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.24450128941208 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 4.521872977874928E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 2.7131237867249566E-4 15 0.0 16 0.0 17 4.521872977874928E-5 18 0.0 19 4.069685680087435E-4 20 2.7131237867249566E-4 21 1.808749191149971E-4 22 6.330622169024899E-4 23 6.782809466812392E-4 24 4.069685680087435E-4 25 4.521872977874928E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2211473.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.28859473394763 #Duplication Level Percentage of deduplicated Percentage of total 1 84.56101246675534 62.81918785435081 2 10.838168442892997 16.10304606224676 3 2.548833525050278 5.680477823602778 4 0.8655870157318211 2.5721297207467364 5 0.3953305113731954 1.4684274072683794 6 0.20689993773024906 0.9222183374712885 7 0.12369698548066532 0.6432492656928906 8 0.0847826587163925 0.503870765907894 9 0.05580418030293556 0.373105272148643 >10 0.2881912980049878 3.6839106101811536 >50 0.016819626187364576 0.8725022237513378 >100 0.012110066195179644 1.8014012691386976 >500 0.0017193776933489846 0.8385028342734848 >1k 9.825015390558219E-4 1.44773004452899 >5k 6.140634619098887E-5 0.27024050869012284 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5924 0.26787575520931073 No Hit GGTATCAACGCAGAGTACTTTTTTT 3668 0.16586230082845235 No Hit TATCAACGCAGAGTACTTTTTTTTT 3550 0.16052649071455993 No Hit GTCCTACAGTGGACATTTCTAAATT 3056 0.1381884382038578 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2925 0.13226478460284163 No Hit CTTTAGGACGTGAAATATGGCGAGG 2772 0.12534631894669301 No Hit CTGTAGGACGTGGAATATGGCAAGA 2750 0.12435150689156052 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.521872977874928E-5 2 0.0 0.0 0.0 0.0 4.521872977874928E-5 3 0.0 0.0 0.0 9.043745955749856E-5 4.521872977874928E-5 4 0.0 0.0 0.0 1.3565618933624783E-4 4.521872977874928E-5 5 0.0 0.0 0.0 1.3565618933624783E-4 4.521872977874928E-5 6 0.0 0.0 0.0 1.3565618933624783E-4 4.521872977874928E-5 7 0.0 0.0 0.0 1.3565618933624783E-4 4.521872977874928E-5 8 0.0 0.0 0.0 1.3565618933624783E-4 4.521872977874928E-5 9 0.0 0.0 0.0 1.3565618933624783E-4 4.521872977874928E-5 10 0.0 0.0 0.0 1.808749191149971E-4 4.521872977874928E-5 11 0.0 0.0 0.0 1.808749191149971E-4 4.521872977874928E-5 12 0.0 0.0 0.0 1.808749191149971E-4 4.521872977874928E-5 13 0.0 0.0 0.0 1.808749191149971E-4 4.521872977874928E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1250 0.0 11.772693 1 AACACCG 85 5.5737233E-5 11.126477 5 GGCGAGG 850 0.0 10.458888 19 GTATAAG 225 1.8189894E-12 10.095896 1 ACTGCGT 75 0.0027481348 10.088233 8 TAGGACC 625 0.0 9.987126 4 TCCAACG 180 4.274625E-10 9.982922 18 CGGTAGG 90 7.802238E-4 9.876419 1 TCGAACT 165 1.1003067E-8 9.744096 19 TGGCGAG 1840 0.0 9.663103 18 AATGTCC 500 0.0 9.646873 8 TAGGACA 655 0.0 9.5297 4 GTCCTAA 1080 0.0 9.510626 1 GTCCTAT 250 1.8189894E-12 9.481362 1 CTTATAC 200 2.8921932E-10 9.457505 3 GTATCAA 2420 0.0 9.427491 1 GGCCTAC 190 5.4333213E-9 9.356607 1 GACGTGG 875 0.0 9.295377 7 GGACGTG 1800 0.0 9.247338 6 TGTCCAC 665 0.0 9.244387 10 >>END_MODULE