FastQCFastQC Report
Thu 26 May 2016
SRR1512146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512146_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2211473
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73170.3308654457911085No Hit
TATCAACGCAGAGTACTTTTTTTTT48730.2203508702118452No Hit
GGTATCAACGCAGAGTACTTTTTTT48130.21763774642512027No Hit
GTCCTACAGTGGACATTTCTAAATT31190.141037218179919No Hit
CTTTAGGACGTGAAATATGGCGAGG30910.13977109374611402No Hit
CTGTAGGACGTGGAATATGGCAAGA30230.13669622012115906No Hit
GTCCTAAAGTGTGTATTTCTCATTT29750.1345257210917791No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22560.10201345438085836No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATC500.001503358713.2959338
GGTATCA14100.012.3095951
ATCCCGT1001.9387753E-611.39599710
AATCCCG2450.011.24145819
GTATCAA28600.010.4702021
GTATAAA3950.010.1401341
TAGGACC5250.010.1355054
GGCGAGG10600.010.12426219
AGGCGTG1356.47211E-79.8488397
TGGCGAG19950.09.71131418
TTAGGAC13750.09.67483
AAGGCGT1401.0632939E-69.4970956
CCAACGA1304.256306E-69.49709419
TCCCGTT1201.7079528E-59.49666511
AGGACGT19350.09.3792715
GTCCTAA10600.09.356621
GAATAGA2550.09.3495641
GTTATAC1951.6771082E-99.2583943
ACTGTTC5850.09.2535798
GGACGTG18800.09.2445126
Produced by FastQC (version 0.11.5)