##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512146_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2211473 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.402404189424878 31.0 30.0 34.0 27.0 34.0 2 30.603293370527247 31.0 31.0 34.0 27.0 34.0 3 30.691709100676338 31.0 31.0 34.0 27.0 34.0 4 34.28615497453507 37.0 35.0 37.0 30.0 37.0 5 33.839445473672974 37.0 35.0 37.0 29.0 37.0 6 33.77795116648496 37.0 35.0 37.0 29.0 37.0 7 33.587000157813364 37.0 35.0 37.0 28.0 37.0 8 33.47174123310572 37.0 35.0 37.0 28.0 37.0 9 34.89406653393462 38.0 35.0 39.0 28.0 39.0 10 34.680729993086054 38.0 35.0 39.0 27.0 39.0 11 34.66163638443698 38.0 35.0 39.0 27.0 39.0 12 34.47245840216001 38.0 35.0 39.0 27.0 39.0 13 34.43579234293161 38.0 35.0 39.0 27.0 39.0 14 35.36066911058828 39.0 35.0 41.0 27.0 41.0 15 35.285591096974734 39.0 35.0 41.0 27.0 41.0 16 35.1707988295584 39.0 34.0 40.0 26.0 41.0 17 35.101926634419684 39.0 34.0 40.0 26.0 41.0 18 35.02512352626508 39.0 34.0 40.0 26.0 41.0 19 35.04683484718104 39.0 34.0 40.0 25.0 41.0 20 34.91870576760376 39.0 34.0 40.0 25.0 41.0 21 34.786796402216986 39.0 34.0 40.0 25.0 41.0 22 34.65447464201462 39.0 34.0 40.0 24.0 41.0 23 34.70598601022938 39.0 34.0 40.0 24.0 41.0 24 34.567752353295745 39.0 34.0 40.0 24.0 41.0 25 34.109876087114785 38.0 34.0 40.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07990856143509006 1101 2 -0.04256910772630107 1101 3 -0.01958225918482981 1101 4 -0.01709223873072574 1101 5 -0.024714641128120718 1101 6 -0.10834182748745746 1101 7 -0.014057448538302708 1101 8 -0.043654281664451844 1101 9 -0.1636964969182486 1101 10 -0.011701675053799931 1101 11 -0.1401544394087182 1101 12 -0.24743415462781115 1101 13 -0.20013754631709446 1101 14 -0.2792574259585834 1101 15 -0.25349371193641446 1101 16 -0.21589670690105578 1101 17 -0.20996870948835067 1101 18 -0.04843545858507525 1101 19 -0.3919072252823028 1101 20 -0.3698417261561957 1101 21 -0.32382349172334557 1101 22 -0.43505678489461275 1101 23 -0.3686127579955283 1101 24 -0.5286005378784182 1101 25 -0.6101413513622518 1102 1 0.20617917950452735 1102 2 0.13586061841525066 1102 3 0.22516010010215837 1102 4 0.2546016616899536 1102 5 0.0629758288006883 1102 6 0.275450999531472 1102 7 0.3814691816054605 1102 8 0.3377164997969544 1102 9 0.48802978616281223 1102 10 0.4506823689342312 1102 11 0.33980939678529154 1102 12 0.4614552341890814 1102 13 0.6124471201869213 1102 14 0.6724252366438748 1102 15 0.6047659260241716 1102 16 0.46407853610092076 1102 17 0.5588752844575922 1102 18 0.41577853313231117 1102 19 0.5868108396160991 1102 20 0.6668581998051764 1102 21 0.8419326370595144 1102 22 0.9225122130912453 1102 23 0.8975572900503721 1102 24 0.6228852516680874 1102 25 0.5588807155302291 1103 1 0.043373232964277975 1103 2 0.19470625132325026 1103 3 0.3058074944307272 1103 4 0.32727325240895766 1103 5 0.35484773198264463 1103 6 0.27387488053570763 1103 7 0.27285196820982804 1103 8 0.24876118817036996 1103 9 0.21038802071465312 1103 10 0.202159016226247 1103 11 0.20307437478783186 1103 12 0.2914008454975132 1103 13 0.10383956975679354 1103 14 0.23019190607138995 1103 15 0.22654426084348955 1103 16 0.364476504117313 1103 17 0.29056579185260745 1103 18 0.38334324658072205 1103 19 0.2716714899093162 1103 20 0.3124533085581973 1103 21 0.37584314987877576 1103 22 0.317887672082108 1103 23 0.22401388059885363 1103 24 0.3983031140252393 1103 25 0.12737713373687853 1104 1 0.3237226873507275 1104 2 0.32904663183244764 1104 3 0.3187165450088969 1104 4 0.15552626772571898 1104 5 0.29175348558043623 1104 6 0.24946350164467646 1104 7 0.37874591906471267 1104 8 0.33323952799872814 1104 9 0.5040019963367897 1104 10 0.355055284223198 1104 11 0.5680816996377445 1104 12 0.6329798214228575 1104 13 0.5385865672066643 1104 14 0.5568147832213697 1104 15 0.5613030674195798 1104 16 0.8200686041310377 1104 17 0.7239188336601003 1104 18 0.7938831311066181 1104 19 0.7989346131997905 1104 20 0.6809056268173421 1104 21 0.7553581458732523 1104 22 0.5789439665059604 1104 23 0.5213577049045881 1104 24 0.504801085728019 1104 25 0.5879542010520922 1105 1 -0.011666268621418396 1105 2 -0.012670036465973311 1105 3 0.0771744744828986 1105 4 0.07837573134457188 1105 5 0.09978102027854163 1105 6 0.08428134340796589 1105 7 0.1837927815108813 1105 8 -0.05969365241586644 1105 9 -0.08350940652173477 1105 10 -0.05612130429018691 1105 11 -0.07539773014939044 1105 12 -0.16555556201623034 1105 13 0.014663098064701785 1105 14 0.05260835224227378 1105 15 -0.08827446347109458 1105 16 -0.177047074857704 1105 17 0.08533816513789816 1105 18 0.0022772667349784115 1105 19 0.10394456690457332 1105 20 0.1877535581406633 1105 21 0.0751525758391054 1105 22 0.03622306963131905 1105 23 -0.11997596721214165 1105 24 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-0.7252840020020415 2315 2 -0.7552670616683983 2315 3 -0.9400602189469822 2315 4 -0.9210537508728933 2315 5 -0.967375559247067 2315 6 -1.0421886368349007 2315 7 -1.0487942498707525 2315 8 -1.0534510464676075 2315 9 -1.184019619329142 2315 10 -1.0174655178010283 2315 11 -0.9836155656989902 2315 12 -1.022969058581971 2315 13 -1.1556770785076722 2315 14 -1.386357398844666 2315 15 -1.4635204981029588 2315 16 -1.5565204064510425 2315 17 -1.5729755932451255 2315 18 -1.4401290653617735 2315 19 -1.3687876946212256 2315 20 -1.3110615346107721 2315 21 -1.5306049505712735 2315 22 -1.549717468622184 2315 23 -1.431779643659219 2315 24 -1.4442204385004445 2315 25 -1.6148941330217212 2316 1 -1.2020278844719456 2316 2 -1.2921823213101042 2316 3 -1.3102451094065053 2316 4 -1.1726872156492334 2316 5 -1.5715437923690345 2316 6 -1.790575361926873 2316 7 -1.6126239963695532 2316 8 -1.738298689544152 2316 9 -2.0121636343559786 2316 10 -2.093315894168022 2316 11 -2.0351319292391494 2316 12 -1.8625365359066635 2316 13 -2.0067049168550497 2316 14 -2.2100357979134273 2316 15 -2.1509198968626038 2316 16 -2.4383908793952287 2316 17 -2.367838698975987 2316 18 -2.3999294711085 2316 19 -2.0390100407674723 2316 20 -2.1623516671955443 2316 21 -2.273363575822259 2316 22 -2.385899487943192 2316 23 -2.330777390748395 2316 24 -2.5338864284584233 2316 25 -2.4104812892021883 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46027.0 3 5.0 4 847.0 5 7201.0 6 8777.0 7 7021.0 8 5740.0 9 5162.0 10 4916.0 11 4549.0 12 4297.0 13 4073.0 14 4085.0 15 4020.0 16 4276.0 17 4418.0 18 4801.0 19 5478.0 20 6743.0 21 8452.0 22 10897.0 23 13781.0 24 16611.0 25 19460.0 26 22617.0 27 25770.0 28 30257.0 29 36518.0 30 46022.0 31 59661.0 32 75731.0 33 96684.0 34 125286.0 35 164140.0 36 236737.0 37 398636.0 38 691777.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.768855665553374 20.669494403550924 14.189718079899457 24.371931850996244 2 14.958988873027163 21.987336042538164 40.32258137449564 22.73109370993903 3 18.212793011716624 26.22600411580878 30.88864299948496 24.672559872989634 4 12.99260718986847 18.654625220384784 38.45373649146971 29.899031098277028 5 13.044367362321019 38.25020664232351 35.93187657470047 12.773549420655003 6 30.397884125196196 38.209781444313364 18.635633353877708 12.756701076612737 7 26.90170759489264 32.39727548109337 23.15746111302286 17.543555810991133 8 24.56908305142153 37.190471689964994 21.19504980806181 17.045395450551666 9 26.055981691840685 15.702610884238696 21.01535944594395 37.22604797797667 10 15.04698452117661 29.409086161124282 34.64098363398513 20.902945683713977 11 33.20773077491789 22.98133416053463 25.430335346621913 18.380599717925563 12 22.90717544369748 25.36494906336184 32.16096239926963 19.56691309367105 13 28.461663334799926 22.597743675821498 26.303373362460224 22.63721962691835 14 20.858540891071247 22.69197950868041 28.197088546864464 28.252391053383874 15 22.515038619056167 31.260793145564065 24.06590539427793 22.158262841101838 16 21.6555210034217 28.694901542998718 28.09457768645604 21.554999767123544 17 21.278306359607374 29.03648382774739 28.273282106541657 21.41192770610358 18 21.121804335843123 27.827380212193408 30.308215384044935 20.74260006791853 19 23.049023070185473 27.316096223822267 28.29996712592399 21.33491358006827 20 23.113508507677913 27.69118139809982 28.69413282459248 20.50117726962979 21 22.666883113653206 27.115185218178112 27.994508637455667 22.223423030713015 22 22.770434004846543 28.173665244839075 28.145946163484698 20.90995458682968 23 22.019034372113065 28.024759967677653 28.37656168535632 21.57964397485296 24 22.52333528236811 27.4022531927315 28.898080856453817 21.176330668446575 25 22.40986214449731 27.674110061923287 28.64908299818309 21.266944795396313 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1053.0 1 1053.0 2 958.5 3 864.0 4 864.0 5 864.0 6 2034.0 7 3204.0 8 3204.0 9 3204.0 10 4524.0 11 5844.0 12 5844.0 13 5844.0 14 9397.0 15 12950.0 16 12950.0 17 12950.0 18 22395.5 19 31841.0 20 31841.0 21 31841.0 22 49263.0 23 66685.0 24 66685.0 25 66685.0 26 93215.5 27 119746.0 28 119746.0 29 119746.0 30 151952.0 31 184158.0 32 184158.0 33 184158.0 34 205358.5 35 226559.0 36 226559.0 37 226559.0 38 240941.0 39 255323.0 40 255323.0 41 255323.0 42 267396.5 43 279470.0 44 279470.0 45 279470.0 46 279556.0 47 279642.0 48 279642.0 49 279642.0 50 267655.5 51 255669.0 52 255669.0 53 255669.0 54 225660.0 55 195651.0 56 195651.0 57 195651.0 58 165987.5 59 136324.0 60 136324.0 61 136324.0 62 109863.5 63 83403.0 64 83403.0 65 83403.0 66 63476.5 67 43550.0 68 43550.0 69 43550.0 70 31282.5 71 19015.0 72 19015.0 73 19015.0 74 13107.5 75 7200.0 76 7200.0 77 7200.0 78 4806.0 79 2412.0 80 2412.0 81 2412.0 82 1548.0 83 684.0 84 684.0 85 684.0 86 434.0 87 184.0 88 184.0 89 184.0 90 109.0 91 34.0 92 34.0 93 34.0 94 20.0 95 6.0 96 6.0 97 6.0 98 4.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40719466165763724 2 0.0 3 0.0 4 0.0 5 0.05118760210954418 6 0.0 7 0.0 8 0.0037983733014149393 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.808749191149971E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005516685033007411 25 0.0012209057040262305 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2211473.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.82354644557371 #Duplication Level Percentage of deduplicated Percentage of total 1 83.84473425063548 61.05870898917938 2 11.27112271885808 16.41606257621045 3 2.6779129494070686 5.8504535414504675 4 0.9154695421732247 2.6667095489583974 5 0.4311000906039716 1.5697118735394682 6 0.22840344741823687 0.9979889436834675 7 0.14631484271752968 0.7458616021021783 8 0.0909425202141135 0.5298205475752016 9 0.06083529522662843 0.3987217752719908 >10 0.2986757921410728 3.7839136934965203 >50 0.017336666711956238 0.8765840835083824 >100 0.013923611049420907 1.9910061756423347 >500 0.0020487116119909505 1.020037900989562 >1k 0.0011174790610854427 1.7636139739246244 >5k 6.208217006030237E-5 0.3308047744675966 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7317 0.3308654457911085 No Hit TATCAACGCAGAGTACTTTTTTTTT 4873 0.2203508702118452 No Hit GGTATCAACGCAGAGTACTTTTTTT 4813 0.21763774642512027 No Hit GTCCTACAGTGGACATTTCTAAATT 3119 0.141037218179919 No Hit CTTTAGGACGTGAAATATGGCGAGG 3091 0.13977109374611402 No Hit CTGTAGGACGTGGAATATGGCAAGA 3023 0.13669622012115906 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2975 0.1345257210917791 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2256 0.10201345438085836 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.521872977874928E-5 7 0.0 0.0 0.0 0.0 4.521872977874928E-5 8 0.0 0.0 0.0 0.0 4.521872977874928E-5 9 0.0 0.0 0.0 0.0 9.043745955749856E-5 10 0.0 0.0 0.0 4.521872977874928E-5 9.043745955749856E-5 11 0.0 0.0 0.0 4.521872977874928E-5 9.043745955749856E-5 12 0.0 0.0 0.0 4.521872977874928E-5 9.043745955749856E-5 13 0.0 0.0 0.0 9.043745955749856E-5 1.3565618933624783E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGATC 50 0.0015033587 13.295933 8 GGTATCA 1410 0.0 12.309595 1 ATCCCGT 100 1.9387753E-6 11.395997 10 AATCCCG 245 0.0 11.241458 19 GTATCAA 2860 0.0 10.470202 1 GTATAAA 395 0.0 10.140134 1 TAGGACC 525 0.0 10.135505 4 GGCGAGG 1060 0.0 10.124262 19 AGGCGTG 135 6.47211E-7 9.848839 7 TGGCGAG 1995 0.0 9.711314 18 TTAGGAC 1375 0.0 9.6748 3 AAGGCGT 140 1.0632939E-6 9.497095 6 CCAACGA 130 4.256306E-6 9.497094 19 TCCCGTT 120 1.7079528E-5 9.496665 11 AGGACGT 1935 0.0 9.379271 5 GTCCTAA 1060 0.0 9.35662 1 GAATAGA 255 0.0 9.349564 1 GTTATAC 195 1.6771082E-9 9.258394 3 ACTGTTC 585 0.0 9.253579 8 GGACGTG 1880 0.0 9.244512 6 >>END_MODULE