FastQCFastQC Report
Thu 26 May 2016
SRR1512145_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512145_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039772
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56360.2763053909946798No Hit
TATCAACGCAGAGTACTTTTTTTTT36740.18011817006998823No Hit
GGTATCAACGCAGAGTACTTTTTTT34930.17124462930170628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12700.013.2864421
CTGCGGA658.324203E-411.641449
TCCAACG2550.011.12757518
GTGTACG802.654024E-411.10117151
GTGCGCC600.00607052411.03511511
TAGGACC4600.010.8977664
CCGTGCA700.001546991810.8099099
TCTATAC2600.010.5497963
CCAACGA1356.067239E-810.50937719
GGACGTG9200.010.3837216
AGGACGT9900.010.2227565
GACGTGG4750.010.1553767
GTAGGAC11050.010.1004143
TGTAGGA11550.09.995862
CTATACT3150.09.908844
TAGGACG10200.09.8293584
GACGTGA4950.09.7450577
GTATCAA24650.09.6075051
TGGCGAG10700.09.54683518
GGCGAGG4600.09.45843919
Produced by FastQC (version 0.11.5)