FastQCFastQC Report
Thu 26 May 2016
SRR1512145_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512145_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039772
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70370.3449895380464091No Hit
TATCAACGCAGAGTACTTTTTTTTT50230.24625301259160337No Hit
GGTATCAACGCAGAGTACTTTTTTT46490.22791763000962856No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22760.11158109827961164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11650.013.4246751
TATTCCG500.001497393213.3028665
ATAGGAC2200.012.9573373
TAGGACC4050.012.9040164
GTCTAGG1158.395473E-710.78031351
TCCAACG1909.094947E-1210.49685718
AATCCCG2550.010.42850619
CCAACGA1201.5294863E-610.28883719
TCTACAC3350.010.2111573
GTATCAA26200.010.1916061
CCAACGT750.002654222510.130548519
ACTGTTC6350.010.0203768
GATATAC6000.010.0132521
AGGACGT10050.09.643875
GTAGGAC9900.09.50204853
CTAAGAC2002.6375346E-109.5020483
CAAGTCG1002.7647632E-49.4969236
TATGTCG900.0011173829.49669116
AGGACCT7950.09.44228655
TGTAGGA11100.09.4164442
Produced by FastQC (version 0.11.5)